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ASPG2 - L-asparaginase 2
UniProt: P00805 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10046
Length: 348 (327)
Sequences: 1650
Seq/Len: 5.05

ASPG2
Paralog alert: 0.36 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: ASPG1 ASPG2
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
121_L 133_M 3.809 1.00
25_N 69_N 3.703 1.00
240_Y 264_A 3.616 1.00
117_T 133_M 3.461 1.00
170_V 182_V 3.363 1.00
163_S 211_Y 3.261 1.00
251_K 281_T 3.253 1.00
125_V 131_V 3.205 1.00
73_E 94_K 3.024 1.00
29_L 75_V 2.634 1.00
95_I 107_I 2.558 1.00
251_K 278_T 2.498 1.00
337_D 340_Q 2.445 1.00
340_Q 343_Q 2.375 1.00
331_A 344_I 2.305 1.00
254_V 282_A 2.291 1.00
260_G 328_L 2.245 1.00
30_A 36_A 2.214 1.00
171_V 176_V 2.189 1.00
78_I 87_V 2.188 1.00
280_A 284_K 2.118 1.00
106_V 158_A 2.104 1.00
25_N 71_K 2.041 1.00
71_K 102_T 2.022 1.00
85_D 244_N 2.018 1.00
290_V 345_F 1.971 1.00
264_A 321_P 1.947 1.00
292_S 316_S 1.941 1.00
176_V 202_G 1.919 1.00
237_G 258_Y 1.864 1.00
134_V 172_M 1.841 1.00
177_L 195_S 1.82 1.00
99_C 105_F 1.811 1.00
177_L 182_V 1.785 1.00
29_L 73_E 1.72 1.00
233_L 259_D 1.716 1.00
291_R 313_F 1.706 1.00
27_T 102_T 1.704 1.00
185_T 192_T 1.69 1.00
288_A 314_V 1.69 1.00
98_D 101_K 1.685 0.99
204_I 209_I 1.67 0.99
76_V 87_V 1.666 0.99
88_W 326_V 1.652 0.99
88_W 243_A 1.641 0.99
290_V 316_S 1.64 0.99
75_V 94_K 1.639 0.99
252_A 256_A 1.621 0.99
249_P 253_L 1.62 0.99
82_D 273_K 1.605 0.99
122_D 180_R 1.599 0.99
290_V 328_L 1.594 0.99
247_D 278_T 1.577 0.99
157_T 176_V 1.544 0.99
99_C 129_K 1.527 0.99
99_C 127_C 1.525 0.99
292_S 321_P 1.512 0.99
265_G 291_R 1.511 0.99
187_T 293_S 1.501 0.99
114_M 170_V 1.49 0.98
247_D 251_K 1.481 0.98
251_K 255_D 1.476 0.98
128_D 165_N 1.456 0.98
185_T 194_K 1.447 0.98
343_Q 347_Q 1.445 0.98
338_P 342_Q 1.433 0.98
92_A 124_T 1.428 0.98
250_A 278_T 1.42 0.98
181_D 213_R 1.418 0.98
153_N 174_D 1.418 0.98
167_G 178_D 1.405 0.98
288_A 341_I 1.388 0.97
235_K 258_Y 1.385 0.97
152_Y 156_V 1.355 0.97
250_A 282_A 1.349 0.97
54_V 72_G 1.339 0.96
241_N 272_Y 1.336 0.96
254_V 287_T 1.326 0.96
29_L 94_K 1.324 0.96
186_N 297_T 1.31 0.96
145_A 148_P 1.306 0.96
85_D 329_Q 1.306 0.96
235_K 248_L 1.299 0.96
128_D 164_A 1.297 0.95
83_M 87_V 1.293 0.95
91_L 107_I 1.278 0.95
109_H 117_T 1.277 0.95
235_K 259_D 1.271 0.95
120_F 124_T 1.271 0.95
52_V 140_S 1.267 0.95
331_A 341_I 1.266 0.95
94_K 97_T 1.26 0.94
105_F 129_K 1.258 0.94
153_N 171_V 1.24 0.94
261_I 282_A 1.238 0.94
115_E 322_Q 1.221 0.93
27_T 98_D 1.209 0.93
239_V 253_L 1.206 0.93
78_I 83_M 1.198 0.92
116_E 294_R 1.197 0.92
342_Q 346_N 1.176 0.91
95_I 105_F 1.175 0.91
338_P 341_I 1.169 0.91
281_T 285_T 1.167 0.91
240_Y 321_P 1.167 0.91
238_I 321_P 1.147 0.90
250_A 275_V 1.145 0.90
32_G 35_I 1.139 0.89
331_A 335_T 1.137 0.89
95_I 131_V 1.115 0.88
84_N 87_V 1.113 0.88
270_N 305_E 1.104 0.87
235_K 246_S 1.103 0.87
93_K 228_S 1.094 0.87
277_D 280_A 1.091 0.87
239_V 250_A 1.089 0.86
114_M 135_G 1.083 0.86
31_T 34_T 1.076 0.86
181_D 318_T 1.071 0.85
163_S 166_R 1.066 0.85
250_A 279_L 1.064 0.85
177_L 200_P 1.063 0.85
283_A 289_V 1.06 0.84
26_I 159_A 1.059 0.84
62_P 152_Y 1.058 0.84
37_G 49_V 1.057 0.84
135_G 172_M 1.049 0.84
260_G 324_A 1.049 0.84
262_V 290_V 1.044 0.83
81_Q 270_N 1.042 0.83
79_G 82_D 1.035 0.82
88_W 120_F 1.029 0.82
47_Y 50_G 1.028 0.82
278_T 281_T 1.028 0.82
237_G 253_L 1.027 0.82
102_T 105_F 1.026 0.82
30_A 74_Q 1.022 0.81
93_K 97_T 1.018 0.81
248_L 278_T 1.016 0.81
93_K 229_K 1.011 0.80
112_D 267_G 1.009 0.80
104_G 130_P 1.006 0.80
106_V 159_A 1.006 0.80
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1wsaA20.91381000.068Contact Map0.821
1nnsA20.93681000.07Contact Map0.735
2wltA10.92821000.072Contact Map0.718
3nxkA80.93391000.078Contact Map0.831
1agxA10.93391000.08Contact Map0.687
4pgaA20.93681000.083Contact Map0.796
1o7jA40.92531000.087Contact Map0.763
2d6fA20.96261000.122Contact Map0.827
1wlsA20.89661000.124Contact Map0.848
2himA40.91951000.124Contact Map0.845

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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