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BTUB - Vitamin B12 transporter BtuB
UniProt: P06129 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10126
Length: 614 (591)
Sequences: 20221
Seq/Len: 34.21

BTUB
Paralog alert: 0.93 [within 20: 0.34] - ratio of genomes with paralogs
Cluster includes: BTUB CIRA FECA FEPA FHUA FHUE FIU YDDB YNCD
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
501_Y 521_Q 4.976 1.00
377_A 389_G 4.536 1.00
457_R 482_K 4.385 1.00
540_Y 565_S 4.276 1.00
409_Y 436_K 3.85 1.00
403_Y 442_F 3.818 1.00
379_S 387_R 3.809 1.00
411_T 436_K 3.624 1.00
567_W 589_F 3.551 1.00
234_W 276_Y 3.165 1.00
379_S 389_G 3.093 1.00
535_D 572_A 3.06 1.00
371_F 397_W 3.057 1.00
184_T 228_H 3.016 1.00
51_R 55_D 2.977 1.00
334_G 361_Y 2.951 1.00
124_V 149_I 2.876 1.00
582_R 609_S 2.834 1.00
449_V 490_F 2.82 1.00
480_R 509_A 2.818 1.00
126_R 150_I 2.802 1.00
403_Y 444_G 2.781 1.00
370_D 397_W 2.735 1.00
503_Y 521_Q 2.667 1.00
456_Y 504_V 2.613 1.00
192_Y 220_K 2.611 1.00
453_I 486_A 2.593 1.00
457_R 480_R 2.562 1.00
50_T 53_D 2.501 1.00
540_Y 567_W 2.459 1.00
498_T 526_Q 2.434 1.00
542_Y 565_S 2.422 1.00
451_W 488_A 2.412 1.00
399_F 405_F 2.376 1.00
69_L 129_Y 2.373 1.00
231_T 234_W 2.355 1.00
545_T 560_K 2.289 1.00
371_F 395_A 2.222 1.00
444_G 451_W 2.216 1.00
537_G 570_A 2.21 1.00
495_L 527_L 2.204 1.00
539_T 568_D 2.196 1.00
158_T 176_T 2.194 1.00
583_G 606_Y 2.182 1.00
178_Q 186_V 2.17 1.00
399_F 403_Y 2.132 1.00
190_G 220_K 2.123 1.00
542_Y 563_G 2.117 1.00
409_Y 438_W 2.116 1.00
579_L 612_Y 2.116 1.00
541_Q 568_D 2.105 1.00
396_G 404_R 2.076 1.00
373_F 395_A 2.067 1.00
375_G 393_T 2.061 1.00
411_T 434_K 2.05 1.00
339_K 356_R 2.047 1.00
584_K 607_T 2.032 1.00
538_I 567_W 2.026 1.00
494_P 529_W 2.012 1.00
237_F 273_G 2.012 1.00
569_L 588_L 1.995 1.00
497_H 527_L 1.993 1.00
235_S 275_R 1.987 1.00
452_R 487_T 1.979 1.00
236_G 274_L 1.965 1.00
51_R 152_T 1.963 1.00
572_A 580_T 1.961 1.00
61_S 64_D 1.96 1.00
48_V 128_E 1.946 1.00
453_I 484_V 1.933 1.00
162_A 172_Y 1.918 1.00
52_Q 56_R 1.917 1.00
440_G 455_G 1.915 1.00
463_L 479_A 1.908 1.00
233_A 276_Y 1.906 1.00
579_L 610_G 1.905 1.00
585_I 588_L 1.895 1.00
450_N 489_N 1.881 1.00
128_E 148_N 1.877 1.00
534_F 573_Y 1.868 1.00
434_K 462_D 1.866 1.00
125_Q 152_T 1.862 1.00
482_K 505_D 1.859 1.00
335_V 358_T 1.857 1.00
581_V 610_G 1.855 1.00
459_D 480_R 1.85 1.00
159_E 613_T 1.846 1.00
431_D 462_D 1.841 1.00
405_F 442_F 1.835 1.00
227_E 235_S 1.827 1.00
372_T 398_E 1.821 1.00
161_S 611_S 1.82 1.00
337_W 358_T 1.819 1.00
161_S 173_D 1.814 1.00
593_Y 601_T 1.81 1.00
100_I 105_L 1.807 1.00
234_W 274_L 1.796 1.00
585_I 606_Y 1.794 1.00
38_P 41_T 1.792 1.00
188_L 224_G 1.79 1.00
459_D 478_K 1.786 1.00
391_W 411_T 1.785 1.00
455_G 482_K 1.778 1.00
100_I 151_T 1.776 1.00
574_P 580_T 1.773 1.00
120_P 123_L 1.753 1.00
71_G 502_D 1.75 1.00
186_V 226_L 1.729 1.00
230_F 236_G 1.727 1.00
456_Y 502_D 1.725 1.00
500_S 524_K 1.721 1.00
394_S 408_S 1.715 1.00
495_L 529_W 1.71 1.00
580_T 611_S 1.708 1.00
101_D 225_A 1.703 1.00
359_G 378_R 1.689 1.00
163_G 609_S 1.678 1.00
125_Q 153_R 1.668 1.00
406_I 441_A 1.658 1.00
439_E 454_S 1.658 1.00
438_W 459_D 1.649 1.00
400_I 403_Y 1.648 1.00
158_T 614_F 1.648 1.00
89_R 485_E 1.648 1.00
406_I 454_S 1.644 1.00
229_N 235_S 1.637 1.00
407_A 440_G 1.632 1.00
194_H 218_L 1.628 1.00
329_G 364_G 1.626 1.00
184_T 226_L 1.624 1.00
575_V 581_V 1.616 1.00
335_V 360_I 1.614 1.00
536_W 571_V 1.605 1.00
568_D 586_A 1.599 1.00
57_W 68_R 1.597 1.00
437_Q 458_N 1.594 1.00
496_T 528_D 1.585 1.00
227_E 237_F 1.585 1.00
52_Q 55_D 1.582 1.00
72_V 88_I 1.581 1.00
414_K 433_E 1.581 1.00
391_W 395_A 1.579 1.00
275_R 282_K 1.576 1.00
585_I 604_R 1.564 1.00
456_Y 483_G 1.563 1.00
499_V 525_Y 1.554 1.00
526_Q 539_T 1.548 1.00
583_G 608_L 1.543 1.00
359_G 380_D 1.535 1.00
406_I 443_E 1.524 1.00
88_I 145_G 1.522 1.00
367_Q 372_T 1.516 1.00
380_D 390_T 1.508 1.00
53_D 56_R 1.502 1.00
183_K 228_H 1.497 1.00
101_D 151_T 1.496 1.00
381_D 387_R 1.494 1.00
185_R 227_E 1.494 1.00
484_V 503_Y 1.491 1.00
399_F 442_F 1.483 1.00
41_T 398_E 1.482 1.00
412_S 435_S 1.478 1.00
455_G 484_V 1.476 1.00
448_G 490_F 1.476 1.00
538_I 569_L 1.47 1.00
443_E 452_R 1.467 1.00
388_H 434_K 1.461 1.00
64_D 67_R 1.449 1.00
275_R 284_Q 1.447 1.00
436_K 461_S 1.435 1.00
331_I 364_G 1.43 1.00
89_R 439_E 1.428 1.00
47_T 131_R 1.424 1.00
541_Q 566_L 1.423 1.00
481_I 506_A 1.423 1.00
492_T 495_L 1.422 1.00
543_L 564_V 1.421 1.00
336_D 359_G 1.421 1.00
442_F 453_I 1.417 1.00
188_L 222_L 1.413 1.00
449_V 488_A 1.407 0.99
190_G 222_L 1.403 0.99
407_A 438_W 1.401 0.99
363_T 374_E 1.394 0.99
465_D 476_E 1.391 0.99
339_K 354_D 1.383 0.99
451_W 486_A 1.376 0.99
489_N 498_T 1.373 0.99
186_V 190_G 1.363 0.99
153_R 225_A 1.363 0.99
581_V 608_L 1.36 0.99
131_R 485_E 1.357 0.99
277_N 282_K 1.347 0.99
574_P 613_T 1.342 0.99
186_V 224_G 1.331 0.99
191_D 221_T 1.33 0.99
491_D 496_T 1.326 0.99
370_D 399_F 1.318 0.99
586_A 605_E 1.315 0.99
161_S 609_S 1.299 0.99
575_V 579_L 1.29 0.99
373_F 393_T 1.286 0.99
235_S 277_N 1.285 0.99
438_W 457_R 1.278 0.99
467_D 474_Y 1.277 0.99
479_A 515_L 1.276 0.99
580_T 613_T 1.275 0.99
528_D 537_G 1.275 0.99
54_I 65_V 1.271 0.99
405_F 440_G 1.261 0.99
70_P 500_S 1.257 0.99
70_P 487_T 1.254 0.99
159_E 611_S 1.253 0.99
534_F 571_V 1.238 0.98
489_N 496_T 1.238 0.98
330_S 367_Q 1.232 0.98
240_G 270_W 1.231 0.98
337_W 356_R 1.231 0.98
330_S 365_L 1.226 0.98
372_T 396_G 1.224 0.98
163_G 171_N 1.223 0.98
238_V 272_A 1.221 0.98
101_D 239_R 1.219 0.98
165_G 169_Y 1.217 0.98
242_G 268_Q 1.209 0.98
566_L 593_Y 1.205 0.98
570_A 582_R 1.204 0.98
461_S 478_K 1.203 0.98
395_A 399_F 1.201 0.98
129_Y 147_V 1.199 0.98
368_V 395_A 1.195 0.98
496_T 530_Q 1.194 0.98
504_V 518_R 1.194 0.98
25_P 28_L 1.193 0.98
69_L 72_V 1.192 0.98
414_K 420_Q 1.187 0.98
240_G 268_Q 1.186 0.98
566_L 601_T 1.185 0.98
395_A 407_A 1.181 0.98
523_V 542_Y 1.181 0.98
398_E 402_G 1.171 0.98
562_G 591_K 1.158 0.97
59_S 65_V 1.152 0.97
433_E 460_V 1.151 0.97
567_W 588_L 1.149 0.97
333_A 362_L 1.148 0.97
521_Q 544_G 1.143 0.97
434_K 461_S 1.14 0.97
502_D 522_Q 1.139 0.97
503_Y 520_K 1.134 0.97
571_V 585_I 1.134 0.97
278_G 283_S 1.127 0.97
466_Y 473_Y 1.121 0.97
514_P 520_K 1.118 0.96
230_F 234_W 1.117 0.96
128_E 150_I 1.115 0.96
138_Y 146_V 1.114 0.96
403_Y 407_A 1.114 0.96
488_A 499_V 1.113 0.96
62_V 149_I 1.111 0.96
528_D 535_D 1.104 0.96
547_Y 560_K 1.103 0.96
501_Y 523_V 1.102 0.96
158_T 162_A 1.101 0.96
366_Q 375_G 1.098 0.96
531_L 536_W 1.096 0.96
478_K 509_A 1.093 0.96
103_V 288_S 1.092 0.96
578_H 612_Y 1.086 0.96
365_L 372_T 1.084 0.96
51_R 125_Q 1.079 0.95
368_V 373_F 1.079 0.95
164_W 170_Q 1.076 0.95
398_E 404_R 1.075 0.95
33_N 37_Q 1.071 0.95
158_T 174_V 1.063 0.95
365_L 374_E 1.059 0.95
569_L 585_I 1.058 0.95
98_V 147_V 1.053 0.95
238_V 270_W 1.048 0.94
160_I 612_Y 1.048 0.94
237_F 275_R 1.047 0.94
456_Y 485_E 1.046 0.94
582_R 611_S 1.046 0.94
143_I 435_S 1.045 0.94
136_A 376_A 1.043 0.94
47_T 487_T 1.043 0.94
435_S 460_V 1.041 0.94
192_Y 218_L 1.038 0.94
88_I 129_Y 1.034 0.94
48_V 126_R 1.033 0.94
360_I 379_S 1.033 0.94
43_L 394_S 1.026 0.94
358_T 381_D 1.019 0.93
579_L 583_G 1.015 0.93
430_L 464_I 1.014 0.93
229_N 232_D 1.013 0.93
159_E 175_S 1.013 0.93
435_S 458_N 1.012 0.93
362_L 377_A 1.012 0.93
377_A 391_W 1.011 0.93
99_L 148_N 1.006 0.93
433_E 463_L 1.005 0.93
391_W 409_Y 1.003 0.93
60_T 64_D 1.003 0.93
445_L 449_V 1.001 0.92
185_R 229_N 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2gufA10.96741000.154Contact Map0.797
4epaA10.9431000.227Contact Map0.746
2hdiA10.92831000.229Contact Map0.797
3qlbA20.95441000.234Contact Map0.804
3efmA10.96091000.236Contact Map0.818
1qfgA10.94631000.239Contact Map0.748
1xkwA10.92351000.241Contact Map0.807
3fhhA10.92511000.242Contact Map0.737
1kmoA10.97721000.245Contact Map0.798
4b7oA10.98371000.256Contact Map0.757

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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