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ASPA - Aspartate ammonia-lyase
UniProt: P0AC38 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10095
Length: 478 (461)
Sequences: 4772
Seq/Len: 10.35

ASPA
Paralog alert: 0.83 [within 20: 0.04] - ratio of genomes with paralogs
Cluster includes: ARLY ASPA FUMC PUR8
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
63_K 73_A 3.562 1.00
346_N 375_S 3.426 1.00
72_V 137_H 3.354 1.00
430_I 434_T 3.309 1.00
400_V 403_G 3.298 1.00
161_I 222_A 3.259 1.00
56_K 81_D 3.151 1.00
60_M 77_I 3.004 1.00
288_A 343_V 2.976 1.00
449_T 452_E 2.934 1.00
200_L 396_A 2.805 1.00
402_E 406_Y 2.737 1.00
183_D 398_K 2.73 1.00
171_R 212_K 2.725 1.00
164_V 215_V 2.721 1.00
224_L 279_M 2.663 1.00
173_G 392_N 2.508 1.00
339_V 382_A 2.476 1.00
160_L 280_V 2.449 1.00
252_K 255_E 2.441 1.00
313_N 395_T 2.436 1.00
168_N 219_Q 2.42 1.00
285_K 347_D 2.412 1.00
177_K 180_E 2.38 1.00
313_N 397_N 2.353 1.00
64_E 252_K 2.317 1.00
176_R 180_E 2.29 1.00
176_R 392_N 2.274 1.00
164_V 219_Q 2.263 1.00
168_N 215_V 2.233 1.00
289_V 344_I 2.206 1.00
22_Y 120_E 2.195 1.00
117_I 121_L 2.18 1.00
157_Y 226_L 2.172 1.00
173_G 387_L 2.113 1.00
63_K 70_K 2.112 1.00
226_L 260_P 2.087 1.00
436_K 444_E 2.07 1.00
60_M 256_V 2.062 1.00
430_I 445_R 2.012 1.00
439_R 454_D 2.01 1.00
169_Q 388_E 2.01 1.00
220_R 223_E 2.003 1.00
315_P 393_G 1.992 1.00
400_V 404_Y 1.973 1.00
69_P 72_V 1.955 1.00
70_K 74_N 1.949 1.00
291_M 336_V 1.948 1.00
215_V 219_Q 1.942 1.00
7_I 16_E 1.925 1.00
220_R 224_L 1.923 1.00
74_N 78_A 1.921 1.00
342_K 371_A 1.886 1.00
173_G 391_I 1.881 1.00
171_R 215_V 1.869 1.00
264_A 270_A 1.85 1.00
418_F 452_E 1.825 1.00
349_T 367_V 1.822 1.00
232_A 270_A 1.799 1.00
387_L 392_N 1.783 1.00
166_A 384_Y 1.777 1.00
169_Q 387_L 1.766 1.00
398_K 402_E 1.765 1.00
452_E 455_D 1.739 1.00
403_G 407_N 1.725 1.00
402_E 460_Q 1.699 1.00
223_E 260_P 1.694 1.00
181_F 184_I 1.69 1.00
450_E 454_D 1.69 1.00
72_V 131_Y 1.678 1.00
60_M 252_K 1.669 1.00
73_A 77_I 1.654 1.00
214_E 290_K 1.65 1.00
333_P 390_C 1.631 1.00
350_V 372_M 1.609 1.00
423_N 426_I 1.602 1.00
248_L 251_K 1.594 1.00
213_E 275_G 1.594 1.00
213_E 269_E 1.568 1.00
230_L 249_A 1.542 1.00
175_E 212_K 1.539 1.00
177_K 391_I 1.517 1.00
166_A 380_T 1.514 1.00
171_R 211_L 1.512 1.00
59_A 68_I 1.505 1.00
458_S 461_N 1.497 1.00
216_K 223_E 1.495 1.00
423_N 447_L 1.487 1.00
159_S 380_T 1.487 1.00
430_I 441_V 1.483 1.00
436_K 441_V 1.483 1.00
228_V 254_A 1.481 1.00
397_N 400_V 1.478 1.00
285_K 351_T 1.475 1.00
175_E 179_V 1.469 1.00
46_E 158_S 1.456 1.00
282_G 286_R 1.453 1.00
254_A 260_P 1.448 1.00
217_N 279_M 1.436 1.00
200_L 204_F 1.428 1.00
23_Y 28_L 1.418 1.00
419_I 424_G 1.416 1.00
64_E 248_L 1.407 0.99
81_D 85_N 1.402 0.99
300_L 358_Q 1.401 0.99
5_I 16_E 1.4 0.99
278_V 286_R 1.389 0.99
82_E 121_L 1.385 0.99
28_L 31_I 1.379 0.99
342_K 374_E 1.379 0.99
7_I 14_T 1.375 0.99
167_I 211_L 1.372 0.99
177_K 181_F 1.366 0.99
429_K 433_E 1.365 0.99
443_L 450_E 1.365 0.99
56_K 77_I 1.358 0.99
312_I 394_I 1.357 0.99
336_V 382_A 1.357 0.99
388_E 392_N 1.357 0.99
204_F 207_F 1.353 0.99
168_N 172_E 1.346 0.99
110_T 114_L 1.335 0.99
61_A 252_K 1.332 0.99
81_D 84_L 1.331 0.99
164_V 222_A 1.33 0.99
295_C 336_V 1.33 0.99
119_L 127_G 1.32 0.99
274_C 365_E 1.317 0.99
179_V 182_Q 1.316 0.99
434_T 441_V 1.313 0.99
75_A 122_M 1.313 0.99
167_I 287_L 1.312 0.99
251_K 255_E 1.306 0.99
52_V 110_T 1.302 0.99
218_I 287_L 1.301 0.99
171_R 208_S 1.3 0.99
399_E 403_G 1.283 0.99
280_V 376_V 1.275 0.99
170_L 391_I 1.275 0.99
280_V 372_M 1.274 0.99
207_F 294_I 1.274 0.99
56_K 60_M 1.271 0.99
9_E 14_T 1.27 0.99
78_A 121_L 1.27 0.99
180_E 395_T 1.267 0.99
178_A 208_S 1.262 0.99
295_C 386_L 1.26 0.99
67_T 140_K 1.25 0.99
207_F 360_Q 1.249 0.99
64_E 245_Y 1.247 0.99
221_T 283_A 1.243 0.98
167_I 215_V 1.243 0.98
63_K 74_N 1.237 0.98
51_M 54_V 1.235 0.98
183_D 201_G 1.227 0.98
53_M 158_S 1.225 0.98
245_Y 256_V 1.224 0.98
283_A 287_L 1.218 0.98
15_R 32_E 1.215 0.98
216_K 224_L 1.213 0.98
51_M 147_A 1.206 0.98
42_S 377_H 1.2 0.98
50_G 158_S 1.196 0.98
53_M 253_L 1.192 0.98
49_R 53_M 1.192 0.98
169_Q 392_N 1.186 0.98
75_A 78_A 1.18 0.98
298_L 333_P 1.178 0.98
451_A 455_D 1.176 0.98
437_S 440_E 1.174 0.98
117_I 120_E 1.166 0.97
227_E 262_V 1.165 0.97
184_I 401_C 1.161 0.97
166_A 387_L 1.16 0.97
57_A 256_V 1.159 0.97
205_R 266_D 1.156 0.97
310_N 400_V 1.154 0.97
156_V 280_V 1.143 0.97
11_L 425_D 1.138 0.97
244_E 248_L 1.133 0.97
64_E 70_K 1.132 0.97
46_E 377_H 1.126 0.97
189_R 194_D 1.126 0.97
63_K 68_I 1.122 0.97
45_P 49_R 1.121 0.97
9_E 422_H 1.118 0.96
56_K 84_L 1.115 0.96
216_K 220_R 1.111 0.96
74_N 77_I 1.109 0.96
277_Y 372_M 1.108 0.96
453_L 457_F 1.101 0.96
119_L 124_H 1.099 0.96
166_A 169_Q 1.097 0.96
248_L 252_K 1.097 0.96
292_S 337_N 1.096 0.96
163_L 379_L 1.088 0.96
46_E 159_S 1.087 0.96
175_E 208_S 1.084 0.96
185_L 463_M 1.076 0.95
344_I 348_T 1.072 0.95
440_E 444_E 1.072 0.95
141_C 242_P 1.068 0.95
191_Q 334_E 1.068 0.95
278_V 354_A 1.066 0.95
65_L 242_P 1.066 0.95
277_Y 350_V 1.062 0.95
208_S 212_K 1.062 0.95
209_I 213_E 1.061 0.95
181_F 396_A 1.061 0.95
160_L 222_A 1.06 0.95
340_C 344_I 1.055 0.95
200_L 401_C 1.055 0.95
52_V 83_V 1.053 0.95
291_M 383_C 1.052 0.95
22_Y 89_C 1.051 0.94
442_V 445_R 1.051 0.94
436_K 440_E 1.045 0.94
173_G 176_R 1.045 0.94
182_Q 201_G 1.043 0.94
164_V 168_N 1.04 0.94
345_G 349_T 1.039 0.94
5_I 19_A 1.035 0.94
175_E 178_A 1.033 0.94
26_H 319_A 1.03 0.94
421_H 425_D 1.027 0.94
287_L 383_C 1.026 0.94
53_M 256_V 1.022 0.93
82_E 117_I 1.02 0.93
174_F 294_I 1.018 0.93
28_L 32_E 1.018 0.93
71_S 75_A 1.012 0.93
52_V 84_L 1.012 0.93
157_Y 259_F 1.009 0.93
169_Q 384_Y 1.007 0.93
430_I 436_K 1.003 0.93
198_M 235_I 1.001 0.92
181_F 398_K 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1jswA411000.078Contact Map0.816
1yfmA10.97071000.082Contact Map0.648
1furA20.97491000.087Contact Map0.712
3r6qA80.97911000.087Contact Map0.819
1vdkA20.97071000.091Contact Map0.719
4hgvA40.96861000.093Contact Map0.749
3e04A40.97071000.101Contact Map0.787
3ocfA40.96231000.102Contact Map0.748
3gtdA20.96231000.106Contact Map0.681
4admA40.96651000.108Contact Map0.734

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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