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ASNB - Asparagine synthetase B [glutamine-hydrolyzing]
UniProt: P22106 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10092
Length: 554 (495)
Sequences: 2058
Seq/Len: 4.16

ASNB
Paralog alert: 0.27 [within 20: 0.02] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
35_W 60_A 3.765 1.00
25_S 47_A 3.538 1.00
106_L 110_K 3.254 1.00
92_Y 109_E 3.215 1.00
242_I 437_F 3.206 1.00
269_H 294_V 3.195 1.00
285_Q 297_E 3.185 1.00
87_E 117_D 3.034 1.00
143_I 402_V 2.966 1.00
215_A 412_V 2.956 1.00
21_A 45_I 2.846 1.00
113_E 116_D 2.84 1.00
336_K 339_A 2.827 1.00
286_E 444_S 2.811 1.00
136_I 146_L 2.694 1.00
106_L 114_F 2.665 1.00
110_K 117_D 2.611 1.00
232_V 244_S 2.587 1.00
59_G 98_S 2.567 1.00
64_Y 71_V 2.513 1.00
435_E 438_E 2.502 1.00
141_L 410_L 2.482 1.00
231_G 342_I 2.472 1.00
125_A 136_I 2.436 1.00
65_N 69_T 2.399 1.00
72_L 100_C 2.381 1.00
218_D 408_K 2.38 1.00
175_F 181_L 2.369 1.00
36_S 49_E 2.347 1.00
393_M 400_A 2.335 1.00
216_L 246_I 2.291 1.00
213_R 439_A 2.257 1.00
114_F 118_L 2.256 1.00
140_H 407_K 2.239 1.00
65_N 108_Q 2.226 1.00
133_A 182_W 2.184 1.00
159_A 164_A 2.171 1.00
217_E 249_K 2.065 1.00
54_V 81_H 2.055 1.00
119_Q 222_S 2.023 1.00
92_Y 105_A 2.019 1.00
115_L 180_Y 2.004 1.00
6_G 158_V 1.986 1.00
30_H 161_E 1.961 1.00
133_A 184_Q 1.956 1.00
269_H 296_H 1.951 1.00
143_I 223_H 1.932 1.00
91_R 109_E 1.914 1.00
121_M 143_I 1.905 1.00
270_S 295_H 1.896 1.00
215_A 409_F 1.887 1.00
241_S 287_V 1.858 1.00
65_N 68_K 1.838 1.00
163_K 317_E 1.828 1.00
284_A 288_A 1.823 1.00
213_R 436_C 1.821 1.00
41_S 129_S 1.817 1.00
86_A 89_G 1.795 1.00
82_Q 225_M 1.791 1.00
431_H 435_E 1.79 1.00
285_Q 289_N 1.785 1.00
334_S 393_M 1.785 1.00
115_L 137_G 1.776 0.99
270_S 293_T 1.776 0.99
114_F 135_L 1.768 0.99
107_Y 126_L 1.755 0.99
107_Y 135_L 1.753 0.99
407_K 411_D 1.729 0.99
355_F 413_A 1.725 0.99
122_F 137_G 1.704 0.99
119_Q 223_H 1.701 0.99
74_V 78_I 1.693 0.99
65_N 105_A 1.685 0.99
219_S 223_H 1.68 0.99
119_Q 408_K 1.679 0.99
209_K 435_E 1.668 0.99
208_D 416_I 1.659 0.99
212_L 355_F 1.657 0.99
6_G 125_A 1.653 0.99
351_S 404_F 1.648 0.99
119_Q 218_D 1.645 0.99
244_S 293_T 1.644 0.99
107_Y 114_F 1.64 0.99
121_M 223_H 1.623 0.99
303_Q 307_D 1.612 0.99
53_I 344_M 1.599 0.99
438_E 447_W 1.584 0.99
127_Y 132_D 1.565 0.98
408_K 411_D 1.565 0.98
72_L 124_F 1.561 0.98
103_I 118_L 1.561 0.98
423_C 431_H 1.559 0.98
69_T 130_E 1.55 0.98
283_A 448_R 1.549 0.98
103_I 114_F 1.542 0.98
126_L 135_L 1.537 0.98
72_L 126_L 1.533 0.98
28_M 164_A 1.528 0.98
443_A 447_W 1.528 0.98
124_F 137_G 1.523 0.98
70_H 104_L 1.519 0.98
222_S 408_K 1.517 0.98
211_E 215_A 1.511 0.98
81_H 225_M 1.503 0.98
74_V 100_C 1.501 0.98
180_Y 189_R 1.488 0.98
136_I 181_L 1.477 0.97
87_E 110_K 1.469 0.97
176_P 179_S 1.453 0.97
119_Q 407_K 1.45 0.97
66_Q 108_Q 1.448 0.97
192_Y 196_W 1.44 0.97
135_L 180_Y 1.424 0.97
174_E 318_T 1.411 0.96
148_M 173_K 1.403 0.96
28_M 49_E 1.399 0.96
128_D 131_K 1.395 0.96
241_S 441_L 1.394 0.96
338_K 343_K 1.38 0.96
115_L 124_F 1.379 0.96
350_G 405_L 1.377 0.96
349_E 388_R 1.374 0.96
2_C 75_N 1.37 0.96
127_Y 134_Y 1.358 0.95
244_S 291_L 1.354 0.95
213_R 440_Y 1.348 0.95
224_L 247_T 1.342 0.95
71_V 129_S 1.34 0.95
212_L 413_A 1.338 0.95
217_E 250_Y 1.332 0.95
67_Q 108_Q 1.329 0.95
222_S 225_M 1.329 0.95
5_F 24_L 1.328 0.94
439_A 443_A 1.325 0.94
54_V 226_S 1.317 0.94
105_A 108_Q 1.317 0.94
311_D 335_R 1.317 0.94
157_Y 168_V 1.315 0.94
333_M 337_I 1.31 0.94
119_Q 406_D 1.305 0.94
69_T 108_Q 1.296 0.93
272_A 284_A 1.292 0.93
70_H 108_Q 1.292 0.93
78_I 103_I 1.289 0.93
271_F 341_G 1.285 0.93
223_H 402_V 1.281 0.93
211_E 412_V 1.28 0.93
106_L 118_L 1.277 0.93
115_L 135_L 1.276 0.93
72_L 104_L 1.259 0.92
215_A 408_K 1.259 0.92
243_I 404_F 1.258 0.92
210_N 439_A 1.251 0.92
151_D 154_G 1.251 0.92
85_R 94_F 1.25 0.92
94_F 102_V 1.244 0.92
247_T 268_L 1.242 0.91
301_T 304_E 1.241 0.91
6_G 136_I 1.24 0.91
112_P 189_R 1.238 0.91
92_Y 108_Q 1.229 0.91
221_K 250_Y 1.224 0.91
121_M 144_I 1.223 0.91
438_E 443_A 1.223 0.91
212_L 412_V 1.222 0.91
232_V 247_T 1.22 0.90
25_S 49_E 1.216 0.90
151_D 155_Q 1.213 0.90
218_D 222_S 1.206 0.90
5_F 25_S 1.205 0.90
156_L 183_S 1.197 0.89
1_M 4_I 1.194 0.89
276_P 299_H 1.19 0.89
37_G 60_A 1.185 0.89
349_E 389_A 1.176 0.88
281_L 297_E 1.174 0.88
389_A 400_A 1.173 0.88
412_V 416_I 1.167 0.88
330_M 389_A 1.155 0.87
330_M 349_E 1.154 0.87
55_D 227_D 1.154 0.87
157_Y 165_L 1.152 0.87
436_C 439_A 1.151 0.87
5_F 47_A 1.15 0.87
79_Y 121_M 1.149 0.87
27_L 167_P 1.148 0.87
205_N 420_D 1.135 0.86
329_P 388_R 1.132 0.86
135_L 182_W 1.129 0.85
204_D 207_T 1.121 0.85
81_H 102_V 1.12 0.85
35_W 48_H 1.119 0.85
137_G 180_Y 1.115 0.84
248_K 291_L 1.103 0.83
75_N 358_Y 1.101 0.83
148_M 156_L 1.098 0.83
213_R 217_E 1.098 0.83
70_H 130_E 1.098 0.83
162_M 172_I 1.086 0.82
310_R 504_E 1.084 0.82
150_Y 173_K 1.081 0.82
315_H 395_A 1.08 0.82
5_F 159_A 1.074 0.81
26_R 49_E 1.065 0.80
147_Y 391_K 1.063 0.80
416_I 432_I 1.053 0.79
54_V 96_T 1.052 0.79
217_E 246_I 1.05 0.79
233_L 337_I 1.047 0.79
112_P 135_L 1.043 0.78
353_E 358_Y 1.043 0.78
211_E 214_Q 1.043 0.78
205_N 435_E 1.042 0.78
466_K 470_A 1.041 0.78
226_S 230_Y 1.04 0.78
474_S 478_L 1.038 0.78
124_F 136_I 1.038 0.78
88_Y 110_K 1.036 0.78
66_Q 105_A 1.026 0.77
316_I 392_A 1.026 0.77
144_I 394_S 1.024 0.77
210_N 214_Q 1.019 0.76
21_A 47_A 1.019 0.76
112_P 182_W 1.018 0.76
88_Y 109_E 1.016 0.76
274_G 281_L 1.013 0.76
335_R 339_A 1.011 0.75
163_K 314_Y 1.011 0.75
39_Y 71_V 1.009 0.75
29_R 49_E 1.008 0.75
59_G 96_T 1.007 0.75
311_D 314_Y 1.007 0.75
63_L 100_C 1.006 0.75
205_N 209_K 1.005 0.75
33_P 51_L 1.004 0.75
3_S 31_R 1.003 0.75
69_T 105_A 1.003 0.75
165_L 172_I 1.001 0.74
233_L 388_R 1 0.74
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1ct9A40.99821000.144Contact Map0.771
1q15A40.83941000.407Contact Map0.599
1jgtA20.8431000.423Contact Map0.594
1ao0A40.69681000.734Contact Map0.567
1xffA20.35561000.75Contact Map0.781
1te5A20.32311000.763Contact Map0.512
2bplA30.7691000.772Contact Map0.719
1ecfA20.66611000.773Contact Map0.694
3mdnA40.35921000.794Contact Map0.603
1ofdA20.80141000.83Contact Map0.312

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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