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ASCB - 6-phospho-beta-glucosidase AscB
UniProt: P24240 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10085
Length: 474 (464)
Sequences: 3220
Seq/Len: 6.94

ASCB
Paralog alert: 0.69 [within 20: 0.13] - ratio of genomes with paralogs
Cluster includes: ASCB BGLA BGLB
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
26_G 109_Q 4.987 1.00
119_E 171_L 4.141 1.00
388_N 457_K 4.11 1.00
403_M 413_L 3.438 1.00
115_R 171_L 3.4 1.00
68_D 71_H 3.248 1.00
74_K 120_E 3.242 1.00
108_Q 112_A 3.202 1.00
81_A 124_Y 3.187 1.00
108_Q 167_A 3.171 1.00
132_L 164_C 3.098 1.00
112_A 116_S 3.093 1.00
120_E 123_K 3.079 1.00
25_E 71_H 2.985 1.00
310_Y 399_H 2.974 1.00
115_R 167_A 2.973 1.00
402_A 405_E 2.967 1.00
246_S 251_D 2.831 1.00
162_R 166_E 2.768 1.00
78_A 124_Y 2.604 1.00
396_L 418_T 2.591 1.00
77_I 117_V 2.588 1.00
455_R 459_F 2.551 1.00
12_G 85_F 2.547 1.00
98_F 163_T 2.533 1.00
222_K 226_E 2.515 1.00
151_R 295_D 2.419 1.00
74_K 116_S 2.409 1.00
224_A 233_V 2.348 1.00
69_F 88_F 2.334 1.00
440_F 454_T 2.331 1.00
139_M 143_T 2.316 1.00
358_N 409_D 2.312 1.00
394_S 398_E 2.309 1.00
354_R 409_D 2.293 1.00
87_V 417_T 2.292 1.00
390_D 457_K 2.287 1.00
109_Q 112_A 2.23 1.00
294_G 297_E 2.229 1.00
155_E 219_L 2.147 1.00
444_D 448_N 2.107 1.00
69_F 113_F 2.04 1.00
122_K 171_L 2.025 1.00
31_T 95_S 2.001 1.00
14_L 19_S 1.993 1.00
90_T 118_F 1.992 1.00
211_H 274_Y 1.988 1.00
119_E 122_K 1.986 1.00
127_E 173_K 1.971 1.00
115_R 119_E 1.971 1.00
162_R 223_I 1.958 1.00
158_S 223_I 1.904 1.00
310_Y 352_G 1.867 1.00
90_T 117_V 1.857 1.00
463_K 467_A 1.843 1.00
118_F 168_F 1.841 1.00
118_F 122_K 1.835 1.00
219_L 298_I 1.832 1.00
23_F 30_L 1.829 1.00
35_M 143_T 1.823 1.00
47_L 61_Y 1.819 1.00
78_A 120_E 1.815 1.00
273_T 300_K 1.81 1.00
10_W 469_N 1.802 1.00
120_E 124_Y 1.792 1.00
121_C 126_I 1.781 1.00
22_A 25_E 1.78 1.00
35_M 139_M 1.777 1.00
77_I 124_Y 1.771 1.00
354_R 358_N 1.765 1.00
464_K 472_D 1.745 1.00
176_L 305_F 1.736 1.00
382_A 386_E 1.732 1.00
92_I 130_V 1.73 1.00
243_Y 355_I 1.715 1.00
276_A 280_R 1.713 1.00
146_G 150_N 1.706 1.00
369_F 414_M 1.693 1.00
381_F 454_T 1.688 1.00
357_M 411_I 1.683 1.00
228_N 231_N 1.677 1.00
22_A 26_G 1.674 1.00
351_L 402_A 1.67 1.00
442_D 450_T 1.653 1.00
30_L 35_M 1.649 1.00
77_I 120_E 1.636 1.00
224_A 231_N 1.632 1.00
127_E 174_Y 1.619 1.00
38_H 64_H 1.613 1.00
162_R 227_V 1.61 1.00
354_R 405_E 1.604 1.00
29_G 101_G 1.598 1.00
87_V 129_L 1.591 1.00
129_L 417_T 1.585 1.00
464_K 467_A 1.581 1.00
153_L 156_F 1.543 0.99
111_I 163_T 1.517 0.99
165_F 224_A 1.514 0.99
170_G 228_N 1.5 0.99
73_Y 116_S 1.5 0.99
158_S 219_L 1.492 0.99
80_M 439_V 1.486 0.99
68_D 72_R 1.479 0.99
77_I 121_C 1.465 0.99
145_Y 156_F 1.456 0.99
72_R 75_E 1.455 0.99
152_K 155_E 1.452 0.99
461_W 465_V 1.449 0.99
90_T 130_V 1.438 0.99
273_T 279_A 1.435 0.99
14_L 90_T 1.42 0.99
80_M 85_F 1.42 0.99
418_T 438_F 1.413 0.99
102_D 144_E 1.412 0.99
115_R 168_F 1.404 0.99
357_M 403_M 1.403 0.99
151_R 212_H 1.4 0.99
398_E 401_R 1.399 0.99
22_A 71_H 1.399 0.99
291_K 296_D 1.393 0.99
176_L 236_M 1.388 0.99
210_A 265_F 1.388 0.99
359_M 363_R 1.383 0.99
400_I 403_M 1.379 0.99
271_R 365_Q 1.375 0.99
82_E 459_F 1.369 0.98
100_Q 103_E 1.368 0.98
462_Y 466_I 1.363 0.98
14_L 88_F 1.362 0.98
223_I 226_E 1.361 0.98
165_F 220_A 1.358 0.98
9_L 87_V 1.351 0.98
132_L 157_F 1.338 0.98
455_R 460_W 1.338 0.98
460_W 463_K 1.328 0.98
5_P 407_I 1.323 0.98
260_R 277_Y 1.319 0.98
140_H 144_E 1.319 0.98
173_K 230_Q 1.315 0.98
264_F 278_S 1.306 0.98
115_R 170_G 1.304 0.98
292_A 295_D 1.303 0.98
373_N 416_Y 1.295 0.98
433_S 445_D 1.263 0.97
244_P 252_V 1.256 0.97
464_K 468_S 1.25 0.97
178_F 213_Q 1.249 0.97
158_S 162_R 1.247 0.97
17_N 63_S 1.247 0.97
161_A 223_I 1.246 0.97
155_E 158_S 1.246 0.97
164_C 168_F 1.245 0.97
118_F 171_L 1.245 0.97
358_N 411_I 1.243 0.97
235_C 269_Q 1.234 0.96
296_D 299_L 1.229 0.96
29_G 140_H 1.225 0.96
78_A 82_E 1.22 0.96
252_V 255_A 1.22 0.96
273_T 365_Q 1.211 0.96
4_F 407_I 1.209 0.96
219_L 223_I 1.208 0.96
111_I 167_A 1.199 0.96
148_W 153_L 1.198 0.96
200_E 205_V 1.193 0.96
273_T 276_A 1.189 0.95
115_R 122_K 1.188 0.95
344_W 376_G 1.185 0.95
149_R 205_V 1.184 0.95
69_F 117_V 1.183 0.95
239_G 356_T 1.177 0.95
353_L 413_L 1.173 0.95
23_F 28_K 1.167 0.95
221_T 225_H 1.156 0.94
4_F 404_G 1.156 0.94
353_L 403_M 1.152 0.94
293_P 296_D 1.145 0.94
152_K 156_F 1.144 0.94
442_D 452_T 1.136 0.94
202_Q 205_V 1.13 0.93
92_I 164_C 1.13 0.93
111_I 115_R 1.127 0.93
29_G 99_P 1.124 0.93
184_M 213_Q 1.124 0.93
158_S 220_A 1.119 0.93
381_F 387_I 1.118 0.93
67_T 422_I 1.116 0.93
92_I 97_L 1.115 0.93
266_I 306_V 1.109 0.93
224_A 228_N 1.108 0.93
404_G 471_E 1.108 0.93
381_F 386_E 1.092 0.92
178_F 216_A 1.091 0.92
404_G 408_A 1.091 0.92
165_F 233_V 1.088 0.92
129_L 369_F 1.086 0.92
118_F 130_V 1.085 0.92
72_R 451_L 1.074 0.91
353_L 370_L 1.064 0.90
65_E 443_R 1.059 0.90
28_K 95_S 1.057 0.90
144_E 152_K 1.055 0.90
443_R 451_L 1.047 0.90
150_N 156_F 1.045 0.89
129_L 174_Y 1.039 0.89
174_Y 232_Q 1.039 0.89
326_S 329_N 1.038 0.89
97_L 163_T 1.037 0.89
239_G 308_F 1.036 0.89
162_R 228_N 1.032 0.89
27_D 107_N 1.029 0.88
78_A 81_A 1.027 0.88
137_V 147_S 1.027 0.88
351_L 355_I 1.027 0.88
160_Y 164_C 1.026 0.88
234_G 307_S 1.024 0.88
163_T 167_A 1.024 0.88
98_F 106_P 1.019 0.88
170_G 231_N 1.018 0.88
343_D 378_K 1.014 0.87
122_K 128_P 1.012 0.87
386_E 457_K 1.01 0.87
177_T 217_S 1.01 0.87
7_S 469_N 1.01 0.87
146_G 149_R 1.009 0.87
108_Q 115_R 1.004 0.87
361_Y 412_P 1.003 0.87
393_I 461_W 1.002 0.87
80_M 126_I 1.002 0.87
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4ddeA20.98521000.079Contact Map0.811
4iplA20.9811000.082Contact Map0.846
3qomA10.9811000.085Contact Map0.798
1v02A50.95361000.095Contact Map0.777
2dgaA10.95151000.097Contact Map0.76
2xhyA40.98311000.102Contact Map0.794
3ptmA20.93881000.103Contact Map0.765
1v08A20.95361000.103Contact Map0.793
2jf7A20.94941000.104Contact Map0.716
3f5lA20.93461000.109Contact Map0.758

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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