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ACON1 - Aconitate hydratase 1
UniProt: P25516 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11325
Length: 891 (885)
Sequences: 1332
Seq/Len: 1.51

ACON1
Paralog alert: 0.17 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: ACON1 YBHJ
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
575_H 581_P 4.458 1.00
452_K 488_Y 4.457 1.00
666_A 750_I 4.281 1.00
288_Y 362_T 4.265 1.00
486_T 490_D 3.713 1.00
785_P 811_I 3.585 1.00
278_H 361_S 3.575 1.00
328_R 334_E 3.54 1.00
741_H 744_D 3.504 1.00
477_S 499_Y 3.491 1.00
452_K 456_T 3.434 1.00
319_I 344_A 3.41 1.00
741_H 760_E 3.336 1.00
253_K 364_E 3.311 1.00
770_K 796_E 3.303 1.00
366_D 369_D 3.259 1.00
576_D 580_D 3.225 1.00
322_V 325_D 3.123 1.00
490_D 495_N 3.104 1.00
87_L 180_C 3.1 1.00
849_V 863_C 3.001 1.00
258_L 267_L 2.987 1.00
455_V 488_Y 2.948 1.00
849_V 861_V 2.902 1.00
847_V 863_C 2.742 1.00
851_L 861_V 2.715 1.00
259_R 262_I 2.7 1.00
713_H 717_M 2.684 1.00
661_G 834_D 2.672 1.00
470_A 502_T 2.601 1.00
341_E 345_K 2.588 1.00
274_M 363_L 2.562 1.00
456_T 488_Y 2.54 1.00
517_T 521_K 2.515 1.00
136_F 512_P 2.456 1.00
731_V 734_V 2.423 1.00
853_R 859_E 2.421 1.00
453_K 593_E 2.42 1.00
792_V 835_I 2.414 1.00
457_L 576_D 2.39 1.00
374_L 389_V 2.371 1.00
224_L 314_C 2.369 1.00
105_E 642_L 2.352 1.00
817_P 846_T 2.319 0.99
371_E 387_P 2.289 0.99
60_E 64_H 2.285 0.99
203_A 339_L 2.277 0.99
834_D 852_T 2.265 0.99
516_E 543_L 2.265 0.99
133_V 468_S 2.248 0.99
250_V 360_T 2.242 0.99
741_H 756_R 2.227 0.99
771_E 774_S 2.208 0.99
527_G 564_M 2.208 0.99
453_K 456_T 2.194 0.99
488_Y 491_E 2.184 0.99
664_I 833_I 2.168 0.99
250_V 361_S 2.15 0.99
450_L 453_K 2.136 0.99
148_L 151_E 2.109 0.99
371_E 385_A 2.089 0.99
158_V 634_Q 2.088 0.99
658_D 837_D 2.088 0.99
739_T 753_A 2.069 0.99
109_R 642_L 2.044 0.98
746_D 756_R 2.037 0.98
660_H 834_D 2.007 0.98
846_T 862_P 1.976 0.98
146_V 176_G 1.971 0.98
683_I 687_S 1.96 0.98
142_F 496_L 1.945 0.98
768_A 795_A 1.93 0.97
659_I 662_A 1.911 0.97
729_E 822_R 1.908 0.97
592_Q 596_R 1.902 0.97
817_P 820_V 1.897 0.97
852_T 858_Q 1.882 0.97
390_P 571_E 1.874 0.97
520_K 543_L 1.874 0.97
734_V 747_V 1.852 0.96
567_N 571_E 1.837 0.96
748_V 752_D 1.826 0.96
457_L 580_D 1.803 0.96
124_D 171_S 1.799 0.96
796_E 821_T 1.797 0.96
446_A 594_I 1.785 0.95
144_E 147_R 1.776 0.95
705_S 708_S 1.767 0.95
567_N 570_S 1.747 0.95
487_P 490_D 1.746 0.95
118_N 169_R 1.745 0.95
374_L 550_A 1.738 0.94
478_D 609_E 1.736 0.94
457_L 582_V 1.734 0.94
373_S 383_R 1.723 0.94
667_M 822_R 1.721 0.94
389_V 564_M 1.709 0.94
253_K 290_D 1.706 0.94
135_R 144_E 1.704 0.94
848_P 860_V 1.701 0.94
250_V 278_H 1.688 0.93
664_I 667_M 1.683 0.93
765_A 833_I 1.676 0.93
828_T 853_R 1.669 0.93
385_A 388_D 1.644 0.92
321_A 325_D 1.635 0.92
450_L 457_L 1.633 0.92
29_A 36_T 1.63 0.92
519_I 526_V 1.629 0.91
252_F 274_M 1.628 0.91
274_M 365_L 1.618 0.91
470_A 497_V 1.617 0.91
684_K 690_G 1.614 0.91
273_Q 383_R 1.606 0.91
16_K 338_E 1.602 0.91
744_D 756_R 1.602 0.91
748_V 753_A 1.602 0.91
851_L 859_E 1.601 0.91
177_T 613_V 1.597 0.90
497_V 502_T 1.595 0.90
312_A 315_G 1.592 0.90
717_M 755_M 1.588 0.90
642_L 647_D 1.583 0.90
293_D 351_R 1.573 0.90
88_M 180_C 1.565 0.89
446_A 553_P 1.561 0.89
575_H 580_D 1.559 0.89
379_R 382_D 1.549 0.89
595_A 598_V 1.549 0.89
161_K 625_V 1.543 0.88
767_I 851_L 1.542 0.88
381_Q 671_S 1.531 0.88
15_A 18_K 1.528 0.88
11_D 27_L 1.525 0.87
699_E 702_D 1.519 0.87
836_G 850_T 1.516 0.87
248_D 361_S 1.51 0.87
732_P 738_M 1.507 0.87
189_G 223_V 1.503 0.86
616_G 621_K 1.503 0.86
304_A 316_F 1.496 0.86
834_D 850_T 1.491 0.86
821_T 824_T 1.485 0.86
850_T 860_V 1.474 0.85
80_A 243_S 1.468 0.85
612_E 615_E 1.466 0.85
53_Q 63_I 1.464 0.84
751_Y 755_M 1.464 0.84
14_Q 17_D 1.463 0.84
273_Q 277_K 1.461 0.84
316_F 319_I 1.46 0.84
812_L 876_Y 1.458 0.84
27_L 30_K 1.458 0.84
455_V 491_E 1.452 0.84
142_F 146_V 1.45 0.84
668_L 726_I 1.445 0.83
392_A 548_W 1.442 0.83
729_E 823_K 1.44 0.83
667_M 729_E 1.431 0.83
452_K 455_V 1.431 0.83
107_V 889_M 1.431 0.83
714_E 717_M 1.42 0.82
596_R 600_Q 1.415 0.82
450_L 557_A 1.413 0.81
150_M 617_T 1.412 0.81
307_S 310_Y 1.41 0.81
617_T 621_K 1.406 0.81
18_K 335_D 1.405 0.81
292_L 351_R 1.404 0.81
389_V 558_Y 1.402 0.81
748_V 756_R 1.402 0.81
563_N 566_I 1.399 0.81
192_V 203_A 1.392 0.80
310_Y 315_G 1.38 0.79
297_L 322_V 1.377 0.79
706_Y 722_A 1.376 0.79
270_T 373_S 1.373 0.79
726_I 730_M 1.371 0.79
878_N 887_R 1.368 0.78
133_V 431_A 1.367 0.78
140_E 144_E 1.357 0.78
69_W 360_T 1.352 0.77
159_F 163_G 1.349 0.77
767_I 794_I 1.349 0.77
189_G 218_I 1.34 0.76
686_D 690_G 1.34 0.76
124_D 190_K 1.335 0.76
474_K 609_E 1.332 0.76
453_K 457_L 1.326 0.75
382_D 671_S 1.323 0.75
365_L 369_D 1.321 0.75
244_M 869_T 1.317 0.75
257_K 294_S 1.312 0.74
743_P 756_R 1.311 0.74
878_N 884_Y 1.311 0.74
505_I 680_A 1.31 0.74
390_P 394_A 1.308 0.74
350_W 464_W 1.308 0.74
84_A 341_E 1.306 0.74
428_V 544_V 1.306 0.74
394_A 565_N 1.299 0.73
731_V 747_V 1.299 0.73
15_A 20_Y 1.299 0.73
431_A 502_T 1.297 0.73
486_T 496_L 1.292 0.73
853_R 857_S 1.292 0.73
453_K 590_S 1.29 0.73
307_S 312_A 1.285 0.72
684_K 687_S 1.285 0.72
450_L 455_V 1.279 0.72
513_D 517_T 1.273 0.71
623_I 626_T 1.27 0.71
668_L 672_V 1.269 0.71
830_E 854_A 1.266 0.70
109_R 648_E 1.266 0.70
381_Q 705_S 1.266 0.70
45_L 83_P 1.261 0.70
218_I 315_G 1.261 0.70
573_I 578_K 1.258 0.70
338_E 342_K 1.257 0.70
641_R 714_E 1.255 0.70
307_S 315_G 1.254 0.69
667_M 823_K 1.254 0.69
49_L 63_I 1.249 0.69
691_R 695_G 1.247 0.69
381_Q 774_S 1.246 0.69
738_M 749_S 1.243 0.68
319_I 350_W 1.238 0.68
344_A 350_W 1.237 0.68
268_V 616_G 1.234 0.68
37_R 358_I 1.232 0.67
22_Y 346_A 1.23 0.67
848_P 862_P 1.228 0.67
727_R 735_E 1.228 0.67
134_D 152_R 1.225 0.67
430_A 494_F 1.224 0.67
794_I 863_C 1.222 0.67
478_D 608_K 1.218 0.66
298_A 329_L 1.216 0.66
820_V 861_V 1.21 0.65
664_I 851_L 1.209 0.65
731_V 738_M 1.207 0.65
143_E 147_R 1.206 0.65
577_R 580_D 1.205 0.65
525_T 546_T 1.2 0.65
388_D 391_K 1.199 0.64
720_T 784_G 1.197 0.64
123_V 237_M 1.197 0.64
730_M 742_L 1.194 0.64
248_D 360_T 1.192 0.64
412_D 419_Q 1.189 0.63
729_E 821_T 1.187 0.63
685_P 690_G 1.186 0.63
61_E 64_H 1.185 0.63
486_T 495_N 1.171 0.62
741_H 762_T 1.171 0.62
455_V 487_P 1.169 0.62
658_D 836_G 1.168 0.61
342_K 346_A 1.167 0.61
430_A 559_A 1.166 0.61
767_I 833_I 1.164 0.61
259_R 371_E 1.16 0.61
150_M 543_L 1.16 0.61
756_R 760_E 1.159 0.61
9_S 24_S 1.158 0.60
334_E 338_E 1.154 0.60
81_Y 142_F 1.153 0.60
663_R 762_T 1.152 0.60
11_D 22_Y 1.152 0.60
11_D 24_S 1.146 0.59
794_I 861_V 1.144 0.59
48_N 88_M 1.143 0.59
604_E 608_K 1.143 0.59
191_A 327_M 1.14 0.59
165_Q 627_R 1.138 0.58
456_T 487_P 1.137 0.58
744_D 760_E 1.135 0.58
78_E 103_M 1.134 0.58
107_V 117_V 1.132 0.58
390_P 565_N 1.127 0.57
826_G 861_V 1.125 0.57
668_L 750_I 1.124 0.57
765_A 792_V 1.124 0.57
255_T 364_E 1.124 0.57
726_I 735_E 1.121 0.57
92_T 680_A 1.12 0.57
516_E 520_K 1.119 0.57
741_H 746_D 1.119 0.57
252_F 365_L 1.118 0.56
662_A 763_P 1.116 0.56
817_P 862_P 1.115 0.56
270_T 365_L 1.114 0.56
450_L 590_S 1.112 0.56
767_I 863_C 1.112 0.56
105_E 109_R 1.111 0.56
850_T 858_Q 1.11 0.56
450_L 574_G 1.109 0.55
374_L 564_M 1.105 0.55
384_V 548_W 1.105 0.55
766_V 790_I 1.101 0.55
867_I 876_Y 1.1 0.55
70_L 73_A 1.099 0.54
29_A 34_D 1.098 0.54
365_L 370_V 1.098 0.54
58_V 463_P 1.098 0.54
304_A 315_G 1.096 0.54
767_I 861_V 1.095 0.54
566_I 571_E 1.094 0.54
236_A 871_T 1.09 0.54
190_K 193_W 1.089 0.53
285_V 310_Y 1.088 0.53
645_F 843_P 1.084 0.53
321_A 341_E 1.082 0.53
96_A 208_L 1.082 0.53
756_R 759_Q 1.079 0.52
730_M 851_L 1.077 0.52
794_I 849_V 1.075 0.52
333_S 336_Q 1.075 0.52
258_L 262_I 1.072 0.52
244_M 268_V 1.072 0.52
92_T 777_S 1.071 0.52
253_K 288_Y 1.069 0.51
97_V 170_F 1.067 0.51
48_N 464_W 1.062 0.51
518_A 522_S 1.06 0.50
494_F 559_A 1.058 0.50
731_V 740_R 1.058 0.50
528_A 540_I 1.058 0.50
391_K 395_A 1.058 0.50
739_T 750_I 1.057 0.50
396_S 548_W 1.054 0.50
30_K 34_D 1.054 0.50
16_K 335_D 1.053 0.50
252_F 287_F 1.052 0.50
57_S 82_R 1.052 0.50
867_I 873_L 1.047 0.49
774_S 785_P 1.046 0.49
618_A 622_G 1.046 0.49
792_V 863_C 1.045 0.49
491_E 495_N 1.045 0.49
180_C 190_K 1.042 0.49
24_S 28_A 1.042 0.49
481_A 486_T 1.039 0.48
451_A 492_L 1.039 0.48
874_T 877_Q 1.039 0.48
180_C 193_W 1.038 0.48
444_L 469_L 1.037 0.48
146_V 150_M 1.037 0.48
352_N 355_D 1.036 0.48
487_P 491_E 1.034 0.48
525_T 545_K 1.033 0.48
631_Y 640_I 1.032 0.48
283_K 361_S 1.031 0.47
64_H 68_G 1.031 0.47
445_M 597_A 1.028 0.47
49_L 53_Q 1.027 0.47
300_R 320_D 1.026 0.47
767_I 822_R 1.023 0.47
381_Q 708_S 1.023 0.47
24_S 347_Q 1.023 0.47
58_V 462_Q 1.023 0.47
83_P 169_R 1.022 0.47
115_A 629_D 1.022 0.47
785_P 788_L 1.022 0.47
420_Y 515_I 1.021 0.46
603_T 607_R 1.021 0.46
185_L 218_I 1.02 0.46
685_P 694_Q 1.017 0.46
83_P 118_N 1.015 0.46
482_K 602_S 1.014 0.46
150_M 548_W 1.014 0.46
768_A 791_R 1.013 0.46
483_A 486_T 1.013 0.46
427_V 527_G 1.013 0.46
304_A 312_A 1.009 0.45
685_P 700_R 1.009 0.45
29_A 32_L 1.008 0.45
304_A 307_S 1.007 0.45
851_L 863_C 1.005 0.45
89_Q 707_G 1.004 0.45
49_L 58_V 1.003 0.45
88_M 96_A 1.002 0.44
541_H 548_W 1.001 0.44
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2b3yA20.98431000.22Contact Map0.691
1c96A10.83161000.446Contact Map0.565
1l5jA20.57011000.692Contact Map0.483
3vbaA60.18631000.902Contact Map0.756
2pkpA10.18181000.903Contact Map0.671
1v7lA20.17961000.905Contact Map0.73
3q3wA20.20541000.911Contact Map0.62
2hcuA10.21551000.912Contact Map0.512
3h5jA20.18741000.915Contact Map0.593
2r48A10.117822.90.992Contact Map0.357

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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