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AMIB - N-acetylmuramoyl-L-alanine amidase AmiB
UniProt: P26365 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11363
Length: 445 (404)
Sequences: 649
Seq/Len: 1.61

AMIB
Paralog alert: 0.52 [within 20: 0.00] - ratio of genomes with paralogs
Cluster includes: AMIA AMIB AMIC
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
348_M 379_V 4.764 1.00
231_N 238_G 3.211 1.00
214_R 217_N 2.924 1.00
266_V 412_L 2.863 1.00
230_L 238_G 2.496 1.00
122_V 126_N 2.493 1.00
230_L 236_F 2.451 1.00
62_D 97_R 2.422 1.00
367_S 372_R 2.36 1.00
213_T 392_E 2.335 1.00
86_R 99_V 2.264 0.99
229_L 406_E 2.229 0.99
300_E 304_K 2.213 0.99
213_T 396_A 2.194 0.99
29_Q 42_S 2.144 0.99
62_D 99_V 2.137 0.99
225_K 401_Q 2.04 0.99
227_R 231_N 2.034 0.99
198_A 223_A 2.026 0.98
62_D 86_R 2.01 0.98
53_H 99_V 2 0.98
223_A 240_L 1.97 0.98
225_K 402_Q 1.931 0.98
29_Q 38_R 1.914 0.98
86_R 97_R 1.894 0.97
209_G 392_E 1.888 0.97
222_I 383_T 1.873 0.97
56_K 99_V 1.85 0.97
233_D 413_R 1.838 0.97
281_A 356_G 1.834 0.97
221_A 401_Q 1.794 0.96
198_A 241_T 1.788 0.96
285_V 345_A 1.737 0.95
58_T 99_V 1.71 0.94
381_V 408_I 1.707 0.94
53_H 56_K 1.694 0.94
271_D 282_S 1.682 0.94
53_H 58_T 1.674 0.94
86_R 101_D 1.672 0.94
58_T 97_R 1.664 0.93
285_V 342_Y 1.632 0.92
279_T 390_S 1.626 0.92
115_N 122_V 1.616 0.92
54_Q 57_R 1.605 0.92
227_R 238_G 1.604 0.92
342_Y 345_A 1.599 0.91
59_V 102_L 1.589 0.91
56_K 97_R 1.582 0.91
264_F 412_L 1.578 0.91
115_N 126_N 1.573 0.91
39_I 100_V 1.57 0.90
236_F 384_G 1.556 0.90
222_I 404_L 1.549 0.90
194_I 264_F 1.546 0.89
92_D 95_T 1.521 0.88
56_K 62_D 1.507 0.88
195_A 241_T 1.503 0.88
58_T 86_R 1.501 0.88
221_A 225_K 1.474 0.86
324_Y 328_A 1.469 0.86
240_L 268_I 1.46 0.86
53_H 62_D 1.459 0.86
272_A 387_S 1.456 0.85
208_I 214_R 1.454 0.85
342_Y 365_H 1.448 0.85
227_R 240_L 1.439 0.84
61_L 100_V 1.433 0.84
400_Y 404_L 1.432 0.84
120_T 124_T 1.408 0.83
31_S 111_V 1.407 0.83
29_Q 40_T 1.396 0.82
56_K 86_R 1.394 0.82
58_T 62_D 1.39 0.82
198_A 244_G 1.389 0.81
258_R 375_D 1.381 0.81
113_R 124_T 1.372 0.80
53_H 60_A 1.362 0.80
273_A 276_R 1.352 0.79
341_G 367_S 1.347 0.79
193_I 237_K 1.339 0.78
113_R 122_V 1.337 0.78
193_I 239_V 1.331 0.77
196_I 238_G 1.329 0.77
341_G 368_L 1.323 0.77
210_P 389_N 1.319 0.77
233_D 409_Y 1.317 0.76
195_A 256_V 1.315 0.76
212_G 352_L 1.309 0.76
334_F 337_S 1.305 0.76
53_H 86_R 1.291 0.74
29_Q 37_A 1.274 0.73
29_Q 111_V 1.269 0.73
283_V 349_I 1.268 0.73
115_N 121_V 1.268 0.73
61_L 98_L 1.268 0.73
29_Q 124_T 1.267 0.72
230_L 409_Y 1.265 0.72
62_D 101_D 1.249 0.71
219_T 240_L 1.249 0.71
43_F 47_P 1.244 0.70
250_V 368_L 1.242 0.70
29_Q 113_R 1.242 0.70
53_H 97_R 1.241 0.70
295_M 334_F 1.228 0.69
56_K 60_A 1.222 0.68
271_D 287_S 1.219 0.68
281_A 384_G 1.217 0.68
345_A 365_H 1.212 0.68
40_T 120_T 1.208 0.67
33_G 203_Q 1.207 0.67
236_F 413_R 1.207 0.67
249_S 252_G 1.204 0.67
337_S 367_S 1.2 0.66
344_V 379_V 1.2 0.66
194_I 408_I 1.2 0.66
29_Q 122_V 1.193 0.66
88_G 99_V 1.19 0.66
31_S 203_Q 1.188 0.65
196_I 227_R 1.179 0.64
59_V 106_G 1.177 0.64
252_G 255_D 1.176 0.64
264_F 344_V 1.172 0.64
209_G 218_V 1.17 0.64
273_A 278_A 1.167 0.63
304_K 341_G 1.166 0.63
120_T 126_N 1.157 0.62
121_V 126_N 1.155 0.62
59_V 407_A 1.151 0.62
299_L 303_E 1.15 0.62
332_L 339_R 1.146 0.61
195_A 238_G 1.143 0.61
334_F 384_G 1.141 0.61
281_A 358_I 1.137 0.60
363_P 368_L 1.135 0.60
271_D 283_V 1.127 0.59
190_D 263_N 1.122 0.59
266_V 381_V 1.119 0.58
111_V 124_T 1.116 0.58
49_Y 61_L 1.116 0.58
229_L 232_D 1.116 0.58
168_T 175_N 1.114 0.58
254_S 367_S 1.112 0.58
285_V 292_N 1.108 0.57
344_V 416_F 1.1 0.57
27_D 42_S 1.1 0.57
299_L 332_L 1.098 0.56
386_I 401_Q 1.097 0.56
91_K 95_T 1.095 0.56
31_S 34_N 1.093 0.56
251_M 294_E 1.093 0.56
298_W 376_I 1.088 0.55
356_G 384_G 1.085 0.55
123_F 126_N 1.082 0.55
367_S 374_P 1.076 0.54
284_W 296_A 1.072 0.54
278_A 359_H 1.066 0.53
338_Q 363_P 1.059 0.52
31_S 38_R 1.059 0.52
211_G 392_E 1.058 0.52
40_T 122_V 1.058 0.52
88_G 101_D 1.054 0.52
292_N 300_E 1.054 0.52
38_R 122_V 1.051 0.51
111_V 126_N 1.044 0.51
300_E 303_E 1.041 0.50
304_K 368_L 1.039 0.50
198_A 219_T 1.034 0.50
359_H 364_E 1.033 0.49
49_Y 110_A 1.029 0.49
337_S 344_V 1.029 0.49
282_S 400_Y 1.025 0.49
166_N 175_N 1.023 0.48
275_N 278_A 1.021 0.48
247_F 250_V 1.017 0.48
391_E 400_Y 1.017 0.48
40_T 124_T 1.016 0.48
304_K 307_E 1.014 0.47
41_L 358_I 1.014 0.47
338_Q 365_H 1.014 0.47
258_R 271_D 1.011 0.47
42_S 122_V 1.01 0.47
121_V 124_T 1.009 0.47
38_R 124_T 1.007 0.47
146_P 159_N 1.006 0.47
285_V 363_P 1.003 0.46
254_S 373_S 1.003 0.46
258_R 282_S 1.001 0.46
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4binA10.88761000.311Contact Map0.549
3ne8A10.52361000.5Contact Map0.594
1jwqA10.41000.583Contact Map0.581
4m6gA10.44271000.59Contact Map0.578
1xovA10.47641000.594Contact Map0.652
3czxA40.39551000.608Contact Map0.621
3qayA40.38431000.624Contact Map0.61
4av2A120.8382990.908Contact Map0.025
4aqzA10.276489.60.962Contact Map0.362
1lj2C20.06295.70.983Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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