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AMY2 - Cytoplasmic alpha-amylase
UniProt: P26612 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11387
Length: 495 (467)
Sequences: 5522
Seq/Len: 11.82

AMY2
Paralog alert: 0.77 [within 20: 0.20] - ratio of genomes with paralogs
Cluster includes: AMY2 GLGX MALZ SUCP TREC
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
89_A 92_R 5.703 1.00
29_D 93_N 5.544 1.00
22_P 82_Q 4.445 1.00
83_L 87_I 4.425 1.00
91_K 230_D 4.232 1.00
79_D 82_Q 4.053 1.00
22_P 85_A 3.989 1.00
84_L 227_T 3.931 1.00
89_A 93_N 3.894 1.00
357_L 422_F 3.892 1.00
353_A 486_V 3.844 1.00
33_D 396_D 3.672 1.00
32_N 93_N 3.515 1.00
357_L 488_V 3.495 1.00
39_V 90_L 3.451 1.00
213_T 247_E 3.193 1.00
25_A 85_A 3.159 1.00
22_P 25_A 3.077 1.00
31_F 36_I 2.894 1.00
84_L 226_Q 2.572 1.00
352_Y 395_L 2.556 1.00
49_S 52_Y 2.553 1.00
46_K 63_D 2.54 1.00
87_I 97_V 2.528 1.00
25_A 89_A 2.503 1.00
81_A 84_L 2.473 1.00
253_Q 289_D 2.461 1.00
214_E 218_Y 2.444 1.00
85_A 89_A 2.437 1.00
446_T 478_T 2.424 1.00
329_A 369_D 2.363 1.00
83_L 229_C 2.308 1.00
437_M 486_V 2.303 1.00
90_L 97_V 2.278 1.00
400_L 404_R 2.271 1.00
222_W 227_T 2.264 1.00
90_L 95_I 2.243 1.00
421_A 436_V 2.234 1.00
393_E 396_D 2.206 1.00
28_A 93_N 2.141 1.00
24_L 86_A 2.116 1.00
80_K 227_T 2.087 1.00
224_M 260_L 2.075 1.00
418_N 440_G 2.064 1.00
86_A 90_L 2.048 1.00
41_L 86_A 2.02 1.00
401_A 461_F 2.005 1.00
62_F 218_Y 1.992 1.00
206_D 209_N 1.988 1.00
372_G 391_I 1.974 1.00
460_D 489_W 1.94 1.00
82_Q 85_A 1.936 1.00
101_V 220_A 1.872 1.00
31_F 95_I 1.858 1.00
31_F 39_V 1.845 1.00
88_D 92_R 1.837 1.00
28_A 90_L 1.831 1.00
41_L 44_A 1.804 1.00
357_L 435_V 1.77 1.00
405_F 490_V 1.758 1.00
249_I 253_Q 1.749 1.00
27_R 30_G 1.731 1.00
460_D 463_G 1.725 1.00
459_R 490_V 1.717 1.00
44_A 83_L 1.715 1.00
46_K 75_T 1.709 1.00
246_K 250_E 1.702 1.00
205_I 212_V 1.69 1.00
83_L 99_L 1.662 1.00
221_R 225_E 1.642 1.00
62_F 219_W 1.642 1.00
106_K 205_I 1.626 1.00
356_L 399_I 1.621 1.00
423_S 434_V 1.616 1.00
482_N 485_S 1.604 1.00
215_E 218_Y 1.601 1.00
457_T 468_R 1.589 1.00
422_F 435_V 1.585 1.00
315_T 358_R 1.564 1.00
316_L 320_D 1.551 1.00
29_D 33_D 1.542 1.00
451_E 477_A 1.538 1.00
459_R 492_E 1.533 1.00
44_A 99_L 1.521 1.00
26_E 89_A 1.52 1.00
81_A 85_A 1.51 1.00
217_K 251_H 1.507 1.00
20_L 23_E 1.497 1.00
221_R 251_H 1.487 1.00
26_E 85_A 1.469 1.00
28_A 86_A 1.469 1.00
106_K 158_K 1.455 1.00
60_D 63_D 1.434 1.00
479_F 489_W 1.425 1.00
37_N 403_Q 1.424 1.00
207_F 216_I 1.415 1.00
243_W 246_K 1.407 0.99
247_E 250_E 1.404 0.99
435_V 488_V 1.385 0.99
46_K 59_Y 1.38 0.99
379_G 382_G 1.378 0.99
80_K 84_L 1.377 0.99
393_E 397_E 1.376 0.99
24_L 41_L 1.37 0.99
448_H 476_E 1.367 0.99
328_V 351_A 1.36 0.99
45_Y 58_S 1.339 0.99
460_D 464_N 1.337 0.99
80_K 222_W 1.335 0.99
405_F 431_P 1.335 0.99
392_I 396_D 1.331 0.99
83_L 86_A 1.324 0.99
36_I 365_V 1.321 0.99
236_A 239_H 1.317 0.99
397_E 400_L 1.307 0.99
61_L 205_I 1.297 0.99
350_L 437_M 1.291 0.99
217_K 247_E 1.291 0.99
22_P 26_E 1.29 0.99
21_W 64_L 1.289 0.99
247_E 251_H 1.286 0.99
327_L 330_N 1.276 0.99
252_V 260_L 1.272 0.99
85_A 88_D 1.249 0.99
26_E 29_D 1.249 0.99
396_D 400_L 1.248 0.99
444_E 480_F 1.244 0.98
365_V 369_D 1.243 0.98
308_M 311_I 1.241 0.98
307_D 311_I 1.24 0.98
45_Y 99_L 1.237 0.98
448_H 478_T 1.232 0.98
103_V 216_I 1.224 0.98
91_K 97_V 1.223 0.98
64_L 83_L 1.222 0.98
365_V 395_L 1.217 0.98
88_D 91_K 1.213 0.98
98_L 231_G 1.21 0.98
419_C 481_C 1.197 0.98
342_P 345_P 1.194 0.98
328_V 369_D 1.193 0.98
215_E 219_W 1.193 0.98
411_T 425_S 1.19 0.98
99_L 229_C 1.189 0.98
220_A 248_W 1.186 0.98
210_H 214_E 1.184 0.98
305_D 308_M 1.179 0.98
433_C 490_V 1.177 0.98
44_A 64_L 1.169 0.97
414_F 434_V 1.163 0.97
245_Y 249_I 1.163 0.97
25_A 29_D 1.163 0.97
119_R 189_Q 1.161 0.97
461_F 490_V 1.161 0.97
469_V 477_A 1.16 0.97
394_Q 464_N 1.159 0.97
217_K 248_W 1.159 0.97
222_W 226_Q 1.156 0.97
100_D 233_R 1.155 0.97
436_V 489_W 1.154 0.97
352_Y 369_D 1.151 0.97
400_L 403_Q 1.147 0.97
58_S 61_L 1.142 0.97
446_T 480_F 1.141 0.97
233_R 332_D 1.126 0.97
235_D 332_D 1.124 0.97
433_C 461_F 1.123 0.97
409_V 425_S 1.115 0.96
310_Q 313_T 1.113 0.96
25_A 86_A 1.111 0.96
436_V 487_S 1.103 0.96
250_E 254_E 1.102 0.96
455_N 471_T 1.098 0.96
36_I 95_I 1.097 0.96
211_A 214_E 1.097 0.96
460_D 487_S 1.093 0.96
310_Q 416_H 1.09 0.96
425_S 428_D 1.087 0.96
210_H 213_T 1.087 0.96
84_L 88_D 1.087 0.96
63_D 80_K 1.085 0.96
352_Y 365_V 1.08 0.95
103_V 219_W 1.08 0.95
412_L 425_S 1.078 0.95
436_V 479_F 1.078 0.95
447_I 450_G 1.075 0.95
46_K 74_P 1.068 0.95
416_H 445_K 1.062 0.95
204_N 239_H 1.06 0.95
87_I 229_C 1.059 0.95
62_F 215_E 1.058 0.95
105_H 331_H 1.058 0.95
401_A 462_L 1.054 0.95
74_P 77_Y 1.05 0.94
436_V 481_C 1.049 0.94
353_A 437_M 1.046 0.94
109_A 180_D 1.036 0.94
297_H 301_R 1.034 0.94
21_W 86_A 1.033 0.94
29_D 89_A 1.032 0.94
86_A 97_V 1.031 0.94
45_Y 219_W 1.031 0.94
213_T 251_H 1.015 0.93
426_G 430_F 1.015 0.93
248_W 262_I 1.012 0.93
273_K 276_T 1.007 0.93
218_Y 221_R 1.007 0.93
401_A 405_F 1.006 0.93
443_G 483_G 1.002 0.92
46_K 60_D 1.002 0.92
25_A 82_Q 1.001 0.92
251_H 255_V 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1hvxA10.97171000.133Contact Map0.682
3bh4A20.96971000.134Contact Map0.685
1wpcA10.96571000.139Contact Map0.7
1ud2A10.96161000.143Contact Map0.709
3bc9A10.92531000.218Contact Map0.747
1mxgA10.83231000.283Contact Map0.769
1wzaA10.84851000.289Contact Map0.734
4e2oA10.83231000.289Contact Map0.781
3zo9A20.88281000.291Contact Map0.768
2wc7A10.84041000.297Contact Map0.778

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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