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CLCA - H(+)/Cl(-) exchange transporter ClcA
UniProt: P37019 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12331
Length: 473 (430)
Sequences: 2559
Seq/Len: 5.95

CLCA
Paralog alert: 0.55 [within 20: 0.02] - ratio of genomes with paralogs
Cluster includes: CLCA CLCB YFEO
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
396_A 428_T 4.222 1.00
334_V 370_A 3.264 1.00
143_M 347_C 3.244 1.00
144_V 344_T 3.13 1.00
138_T 143_M 3.081 1.00
155_G 181_G 3.05 1.00
145_L 347_C 2.743 1.00
144_V 340_R 2.74 1.00
251_T 389_F 2.705 1.00
392_A 428_T 2.7 1.00
198_L 410_I 2.57 1.00
271_K 275_G 2.451 1.00
49_A 158_I 2.387 1.00
261_I 367_L 2.295 1.00
51_V 232_F 2.16 1.00
138_T 145_L 2.155 1.00
255_Y 392_A 2.117 1.00
221_G 225_S 2.106 1.00
258_L 371_F 2.07 1.00
301_L 346_L 1.983 1.00
265_F 397_L 1.975 1.00
50_A 151_T 1.965 1.00
337_F 363_L 1.947 1.00
95_F 99_K 1.891 1.00
259_G 263_G 1.87 1.00
101_A 104_A 1.835 1.00
320_I 365_T 1.829 1.00
263_G 436_A 1.767 1.00
138_T 347_C 1.755 1.00
266_G 400_A 1.754 1.00
259_G 432_A 1.739 1.00
305_L 343_T 1.733 1.00
275_G 278_D 1.723 1.00
59_W 63_Q 1.698 1.00
276_M 349_S 1.681 1.00
56_G 136_L 1.666 1.00
263_G 435_L 1.659 1.00
280_L 350_S 1.658 1.00
256_L 260_I 1.64 1.00
253_W 257_I 1.637 1.00
333_L 370_A 1.633 1.00
398_L 402_I 1.631 1.00
138_T 352_A 1.623 1.00
265_F 367_L 1.622 1.00
275_G 279_L 1.602 0.99
403_R 437_Q 1.597 0.99
306_G 313_S 1.589 0.99
150_P 190_F 1.586 0.99
90_A 94_Y 1.584 0.99
102_P 106_G 1.584 0.99
60_L 140_G 1.58 0.99
55_K 59_W 1.58 0.99
184_A 221_G 1.575 0.99
42_V 177_L 1.559 0.99
360_M 401_S 1.536 0.99
183_A 200_I 1.527 0.99
337_F 370_A 1.526 0.99
269_F 344_T 1.525 0.99
399_A 429_G 1.501 0.99
179_A 205_R 1.474 0.99
302_C 305_L 1.446 0.99
180_T 221_G 1.427 0.98
128_L 157_N 1.424 0.98
262_F 367_L 1.423 0.98
183_A 187_A 1.421 0.98
330_M 370_A 1.407 0.98
44_T 48_L 1.406 0.98
409_I 426_I 1.405 0.98
179_A 218_V 1.392 0.98
319_L 362_A 1.39 0.98
337_F 366_V 1.383 0.98
255_Y 428_T 1.383 0.98
374_V 378_L 1.381 0.98
53_F 135_G 1.378 0.98
260_I 431_G 1.378 0.98
199_F 203_E 1.375 0.98
361_L 394_M 1.373 0.98
104_A 130_V 1.369 0.98
312_T 343_T 1.366 0.98
91_M 94_Y 1.359 0.98
412_V 425_M 1.349 0.98
50_A 188_A 1.343 0.97
134_G 353_P 1.33 0.97
398_L 408_G 1.329 0.97
44_T 228_M 1.327 0.97
80_T 88_V 1.326 0.97
371_F 375_A 1.326 0.97
254_L 375_A 1.32 0.97
194_L 410_I 1.316 0.97
400_A 432_A 1.301 0.97
197_I 219_F 1.301 0.97
143_M 352_A 1.289 0.97
48_L 232_F 1.28 0.96
45_L 162_V 1.267 0.96
64_R 141_G 1.266 0.96
112_I 156_G 1.266 0.96
45_L 158_I 1.262 0.96
86_S 89_L 1.261 0.96
161_M 165_I 1.255 0.96
333_L 366_V 1.254 0.96
131_K 353_P 1.254 0.96
40_A 224_M 1.253 0.96
341_V 363_L 1.248 0.96
108_G 149_G 1.248 0.96
304_L 308_V 1.245 0.96
45_L 49_A 1.241 0.95
304_L 307_F 1.241 0.95
260_I 435_L 1.239 0.95
106_G 131_K 1.227 0.95
47_G 51_V 1.226 0.95
425_M 429_G 1.225 0.95
323_A 366_V 1.224 0.95
41_V 44_T 1.223 0.95
258_L 262_F 1.214 0.95
402_I 415_M 1.213 0.95
262_F 397_L 1.212 0.95
290_K 294_M 1.211 0.95
107_S 110_P 1.211 0.95
163_L 174_R 1.204 0.94
337_F 367_L 1.195 0.94
264_I 268_I 1.183 0.94
113_E 446_S 1.18 0.94
333_L 369_T 1.17 0.93
254_L 371_F 1.165 0.93
276_M 345_L 1.164 0.93
131_K 185_G 1.162 0.93
268_I 341_V 1.16 0.93
131_K 150_P 1.16 0.93
182_A 204_M 1.159 0.93
272_W 345_L 1.157 0.93
187_A 222_V 1.156 0.93
89_L 137_G 1.155 0.93
442_K 450_A 1.153 0.93
131_K 149_G 1.145 0.92
263_G 432_A 1.144 0.92
407_T 411_L 1.142 0.92
249_L 253_W 1.141 0.92
97_V 104_A 1.14 0.92
215_I 219_F 1.14 0.92
87_A 91_M 1.139 0.92
219_F 430_L 1.136 0.92
49_A 128_L 1.131 0.92
58_A 62_N 1.128 0.91
101_A 130_V 1.127 0.91
40_A 220_I 1.126 0.91
185_G 263_G 1.123 0.91
54_D 147_R 1.119 0.91
331_G 335_F 1.114 0.91
231_I 339_A 1.112 0.91
92_F 137_G 1.11 0.91
91_M 296_G 1.105 0.90
151_T 407_T 1.104 0.90
151_T 358_A 1.103 0.90
132_F 154_I 1.1 0.90
46_V 155_G 1.097 0.90
52_A 132_F 1.097 0.90
95_F 100_Y 1.092 0.90
129_P 133_F 1.086 0.89
278_D 451_R 1.086 0.89
189_A 203_E 1.086 0.89
391_I 416_T 1.085 0.89
26_L 29_D 1.083 0.89
267_P 436_A 1.082 0.89
256_L 431_G 1.081 0.89
316_G 362_A 1.078 0.89
83_F 86_S 1.074 0.89
175_H 266_G 1.074 0.89
262_F 400_A 1.072 0.88
39_A 220_I 1.072 0.88
91_M 95_F 1.069 0.88
387_G 390_A 1.069 0.88
93_G 138_T 1.067 0.88
403_R 436_A 1.066 0.88
413_L 422_I 1.064 0.88
92_F 95_F 1.063 0.88
136_L 140_G 1.061 0.88
133_F 341_V 1.058 0.88
353_P 356_I 1.054 0.87
372_G 386_A 1.053 0.87
239_I 358_A 1.051 0.87
89_L 141_G 1.051 0.87
183_A 218_V 1.05 0.87
127_V 153_Q 1.05 0.87
203_E 348_F 1.047 0.87
145_L 354_G 1.046 0.87
79_L 82_A 1.042 0.86
360_M 397_L 1.042 0.86
185_G 189_A 1.04 0.86
109_I 152_V 1.039 0.86
280_L 320_I 1.039 0.86
218_V 222_V 1.034 0.86
266_G 401_S 1.033 0.86
147_R 153_Q 1.032 0.86
42_V 46_V 1.029 0.86
344_T 363_L 1.029 0.86
155_G 159_G 1.028 0.85
192_A 234_H 1.026 0.85
139_L 150_P 1.024 0.85
196_G 200_I 1.02 0.85
253_W 256_L 1.018 0.85
162_V 166_F 1.015 0.84
57_V 139_L 1.014 0.84
339_A 342_I 1.012 0.84
59_W 62_N 1.011 0.84
344_T 347_C 1.01 0.84
371_F 389_F 1.006 0.84
395_G 429_G 1.003 0.83
112_I 180_T 1.003 0.83
195_A 407_T 1 0.83
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4eneA20.94081000.19Contact Map0.737
3nd0A20.97891000.197Contact Map0.709
3orgA40.91971000.323Contact Map0.661
1jcnA20.236898.90.891Contact Map0.114
3usbA20.230466.90.962Contact Map0.035
2ksfA10.217820.30.972Contact Map0.199
4avfA40.196615.40.974Contact Map0
1rh5B10.152212.40.975Contact Map0.299
2wwbB10.14164.80.979Contact Map0.387
3bo0B10.13114.20.98Contact Map0.221

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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