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BTUF - Vitamin B12-binding protein
UniProt: P37028 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12334
Length: 266 (243)
Sequences: 5971
Seq/Len: 24.57

BTUF
Paralog alert: 0.78 [within 20: 0.19] - ratio of genomes with paralogs
Cluster includes: BTUF FECB FEPB FHUD
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
123_A 132_A 5.007 1.00
120_R 133_E 4.664 1.00
208_S 232_L 4.266 1.00
116_A 140_L 3.823 1.00
128_Q 131_K 3.587 1.00
124_P 129_P 3.29 1.00
142_Q 256_Q 3.17 1.00
28_T 34_T 2.987 1.00
120_R 136_A 2.913 1.00
133_E 137_Q 2.706 1.00
137_Q 141_D 2.681 1.00
180_L 185_G 2.573 1.00
73_R 76_A 2.566 1.00
131_K 134_Q 2.49 1.00
157_R 186_E 2.435 1.00
144_A 148_A 2.408 1.00
25_R 45_V 2.382 1.00
231_Q 234_I 2.165 1.00
27_I 74_I 2.136 1.00
177_N 181_E 2.132 1.00
140_L 144_A 2.102 1.00
36_L 119_L 2.071 1.00
203_Q 206_A 2.042 1.00
26_V 126_S 2.032 1.00
37_A 126_S 1.971 1.00
96_Q 99_S 1.928 1.00
210_Q 232_L 1.869 1.00
134_Q 138_S 1.854 1.00
141_D 145_Q 1.788 1.00
83_I 122_L 1.732 1.00
120_R 140_L 1.726 1.00
105_M 125_W 1.722 1.00
28_T 37_A 1.675 1.00
211_A 237_I 1.673 1.00
69_M 74_I 1.671 1.00
145_Q 149_Q 1.669 1.00
94_V 98_A 1.65 1.00
144_A 147_K 1.644 1.00
114_Q 117_N 1.638 1.00
37_A 44_P 1.624 1.00
46_G 77_L 1.581 1.00
228_W 234_I 1.572 1.00
23_A 80_D 1.552 1.00
211_A 235_P 1.538 1.00
27_I 46_G 1.536 1.00
105_M 121_Q 1.533 1.00
157_R 210_Q 1.523 1.00
95_D 98_A 1.5 1.00
81_L 105_M 1.495 1.00
95_D 99_S 1.491 1.00
116_A 136_A 1.49 1.00
159_F 169_T 1.474 1.00
27_I 77_L 1.459 1.00
213_V 258_L 1.454 1.00
142_Q 253_L 1.446 1.00
146_L 255_A 1.44 1.00
117_N 120_R 1.435 1.00
75_V 100_L 1.418 1.00
72_E 202_E 1.416 1.00
130_D 134_Q 1.392 0.99
61_E 73_R 1.377 0.99
143_Y 255_A 1.366 0.99
138_S 142_Q 1.359 0.99
226_Q 229_G 1.358 0.99
38_F 57_A 1.355 0.99
40_A 128_Q 1.346 0.99
24_P 80_D 1.342 0.99
188_I 207_R 1.331 0.99
185_G 262_L 1.331 0.99
155_K 186_E 1.33 0.99
131_K 135_A 1.328 0.99
158_V 185_G 1.322 0.99
203_Q 207_R 1.32 0.99
70_N 73_R 1.31 0.99
237_I 261_A 1.29 0.99
84_A 106_W 1.273 0.99
91_E 95_D 1.273 0.99
45_V 77_L 1.262 0.99
148_A 151_A 1.258 0.99
211_A 262_L 1.256 0.99
117_N 121_Q 1.254 0.99
136_A 140_L 1.253 0.99
228_W 231_Q 1.244 0.98
130_D 133_E 1.238 0.98
48_S 51_S 1.219 0.98
129_P 133_E 1.217 0.98
137_Q 140_L 1.212 0.98
189_F 207_R 1.21 0.98
121_Q 125_W 1.198 0.98
191_D 207_R 1.168 0.97
98_A 104_V 1.158 0.97
44_P 60_I 1.153 0.97
37_A 83_I 1.152 0.97
55_P 58_Q 1.145 0.97
135_A 138_S 1.119 0.96
38_F 44_P 1.115 0.96
119_L 132_A 1.113 0.96
258_L 262_L 1.104 0.96
75_V 102_I 1.097 0.96
212_I 233_K 1.093 0.96
35_E 250_R 1.072 0.95
133_E 136_A 1.067 0.95
159_F 204_V 1.062 0.95
121_Q 124_P 1.057 0.95
138_S 141_D 1.053 0.95
71_L 96_Q 1.041 0.94
156_K 211_A 1.038 0.94
158_V 180_L 1.037 0.94
143_Y 182_V 1.037 0.94
28_T 44_P 1.031 0.94
43_T 128_Q 1.031 0.94
156_K 235_P 1.023 0.93
141_D 144_A 1.019 0.93
143_Y 147_K 1.014 0.93
145_Q 148_A 1.006 0.93
232_L 235_P 1.004 0.93
47_V 60_I 1.001 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1n2zA20.92111000.23Contact Map0.721
2r7aA40.90981000.258Contact Map0.868
2r79A10.91351000.261Contact Map0.711
4hn9A20.99251000.263Contact Map0.789
2q8pA10.91731000.268Contact Map0.787
3md9A20.9061000.272Contact Map0.773
4m7oA10.96621000.273Contact Map0.737
3pshA10.96991000.304Contact Map0.763
4mlzA20.96991000.306Contact Map0.688
4ljsA10.98121000.315Contact Map0.765

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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