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MLAF - Probable phospholipid import ATP-binding protein MlaF
UniProt: P63386 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12801
Length: 269 (240)
Sequences: 68779
Seq/Len: 286.58

MLAF
Paralog alert: 0.94 [within 20: 0.84] - ratio of genomes with paralogs
Cluster includes: ARTP BTUD CCMA CYSA DDPD DDPF DPPD DPPF FBPC FECE FEPC FHUC FTSE GLNQ GLTL HISP LIVF LIVG LOLD LPTB MALK METN MLAF MODC NIKD NIKE OPPD OPPF PHNC PHNK PHNL POTA POTG PROV PSTB SAPD SAPF SSUB SUFC TAUB THIQ UGPC YADG YBBA YBBL YCJV YDCT YECC YEHX YHDZ YNJD ZNUC
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
38_A 212_A 3.294 1.00
10_D 30_T 3.099 1.00
35_K 214_H 3.057 1.00
137_A 140_L 3.003 1.00
64_E 71_N 2.922 1.00
12_R 64_E 2.912 1.00
13_D 26_N 2.872 1.00
86_R 164_D 2.745 1.00
37_T 216_W 2.718 1.00
22_C 25_D 2.697 1.00
10_D 66_L 2.646 1.00
130_E 135_R 2.6 1.00
25_D 222_K 2.501 1.00
32_P 35_K 2.362 1.00
214_H 226_H 2.325 1.00
15_S 26_N 2.267 1.00
11_M 14_V 2.261 1.00
227_G 231_A 2.252 1.00
124_T 162_E 2.242 1.00
17_T 22_C 2.231 1.00
68_D 164_D 2.186 1.00
16_F 53_L 2.184 1.00
102_V 145_L 2.137 1.00
15_S 22_C 2.11 1.00
15_S 62_H 2.036 1.00
126_M 138_A 2.035 1.00
228_S 231_A 2.019 1.00
129_L 138_A 2.013 1.00
103_F 139_K 1.995 1.00
216_W 223_I 1.935 1.00
103_F 138_A 1.875 1.00
12_R 66_L 1.864 1.00
189_E 193_A 1.862 1.00
33_R 164_D 1.809 1.00
103_F 126_M 1.794 1.00
13_D 28_S 1.77 1.00
35_K 213_D 1.735 1.00
18_R 23_I 1.72 1.00
162_E 196_V 1.715 1.00
16_F 61_D 1.676 1.00
131_A 189_E 1.671 1.00
216_W 226_H 1.643 1.00
103_F 125_V 1.615 1.00
117_P 120_L 1.597 1.00
124_T 127_M 1.559 1.00
127_M 131_A 1.55 1.00
122_H 126_M 1.525 1.00
140_L 144_E 1.508 1.00
191_N 213_D 1.482 1.00
131_A 194_L 1.481 1.00
92_Q 147_G 1.471 1.00
131_A 193_A 1.469 1.00
17_T 61_D 1.459 1.00
103_F 122_H 1.448 1.00
51_L 201_V 1.43 1.00
66_L 71_N 1.409 1.00
123_S 127_M 1.395 0.99
16_F 58_I 1.393 0.99
103_F 107_A 1.39 0.99
24_F 53_L 1.386 0.99
12_R 30_T 1.379 0.99
29_L 216_W 1.377 0.99
107_A 121_L 1.358 0.99
11_M 54_I 1.357 0.99
227_G 232_L 1.354 0.99
107_A 125_V 1.343 0.99
31_V 37_T 1.34 0.99
68_D 86_R 1.333 0.99
202_S 208_V 1.331 0.99
135_R 138_A 1.327 0.99
53_L 60_P 1.32 0.99
37_T 226_H 1.312 0.99
8_L 68_D 1.294 0.99
15_S 25_D 1.285 0.99
11_M 29_L 1.283 0.99
168_F 187_I 1.282 0.99
106_V 160_A 1.281 0.99
124_T 128_K 1.24 0.98
102_V 153_A 1.218 0.98
101_N 104_D 1.218 0.98
12_R 28_S 1.215 0.98
36_I 191_N 1.214 0.98
71_N 74_A 1.207 0.98
14_V 60_P 1.191 0.98
126_M 130_E 1.178 0.98
105_N 157_R 1.169 0.97
188_S 191_N 1.153 0.97
54_I 199_V 1.146 0.97
137_A 149_M 1.142 0.97
51_L 167_M 1.139 0.97
52_R 58_I 1.134 0.97
126_M 135_R 1.129 0.97
128_K 160_A 1.122 0.97
191_N 198_C 1.122 0.97
32_P 214_H 1.121 0.97
237_D 240_V 1.113 0.96
11_M 50_L 1.113 0.96
186_L 189_E 1.099 0.96
33_R 68_D 1.087 0.96
50_L 218_L 1.083 0.96
36_I 198_C 1.082 0.96
27_I 50_L 1.08 0.95
180_M 207_E 1.072 0.95
107_A 122_H 1.069 0.95
162_E 194_L 1.067 0.95
145_L 153_A 1.064 0.95
16_F 49_T 1.062 0.95
18_R 49_T 1.062 0.95
53_L 65_I 1.062 0.95
132_V 156_A 1.053 0.95
76_S 79_R 1.048 0.94
164_D 196_V 1.047 0.94
120_L 123_S 1.046 0.94
55_G 89_M 1.045 0.94
37_T 214_H 1.041 0.94
168_F 198_C 1.033 0.94
190_L 196_V 1.032 0.94
224_V 237_D 1.03 0.94
13_D 62_H 1.021 0.93
55_G 167_M 1.019 0.93
184_V 207_E 1.017 0.93
90_L 155_L 1.015 0.93
39_I 199_V 1.01 0.93
127_M 162_E 1.009 0.93
129_L 134_L 1.009 0.93
238_P 242_Q 1.006 0.93
104_D 107_A 1.004 0.93
127_M 130_E 1.002 0.92
166_I 198_C 1.001 0.92
141_M 144_E 1.001 0.92
90_L 168_F 1 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3tuiC40.99631000.087Contact Map0.879
2it1A20.95911000.093Contact Map0.864
2yyzA10.95911000.096Contact Map0.809
1v43A10.9741000.096Contact Map0.825
1g29120.9741000.098Contact Map0.895
1oxxK10.97771000.099Contact Map0.804
3rlfA20.95911000.103Contact Map0.832
3fvqA20.97771000.107Contact Map0.774
1z47A20.97771000.109Contact Map0.833
3d31A20.93681000.119Contact Map0.815

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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