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AMID - N-acetylmuramoyl-L-alanine amidase AmiD
UniProt: P75820 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13687
Length: 276 (275)
Sequences: 389
Seq/Len: 1.41

AMID
Paralog alert: 0.10 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
48_V 164_V 4.45 1.00
142_I 182_P 3.273 1.00
74_V 145_L 3.111 1.00
120_L 145_L 2.993 1.00
184_Q 188_Q 2.875 1.00
192_G 248_H 2.712 1.00
47_L 149_A 2.707 1.00
40_A 95_E 2.595 1.00
136_P 180_L 2.492 1.00
219_L 238_Q 2.453 1.00
139_P 185_Q 2.039 0.98
225_Y 249_F 2.022 0.98
103_S 169_I 2.01 0.98
225_Y 248_H 1.991 0.98
74_V 120_L 1.981 0.98
193_A 225_Y 1.909 0.97
93_E 152_I 1.846 0.96
179_P 247_M 1.843 0.96
261_E 265_I 1.829 0.96
54_D 62_T 1.739 0.94
49_I 145_L 1.731 0.94
191_I 248_H 1.7 0.93
164_V 169_I 1.693 0.93
221_L 267_E 1.65 0.91
193_A 249_F 1.628 0.90
47_L 153_I 1.617 0.90
235_P 238_Q 1.602 0.90
195_P 200_V 1.569 0.88
134_F 137_F 1.557 0.88
250_R 259_D 1.536 0.87
47_L 163_V 1.532 0.87
149_A 163_V 1.522 0.86
152_I 155_R 1.519 0.86
111_L 169_I 1.487 0.84
75_P 88_W 1.474 0.84
246_Q 256_G 1.458 0.83
215_T 238_Q 1.453 0.82
246_Q 262_T 1.453 0.82
215_T 219_L 1.443 0.82
110_R 113_D 1.442 0.82
106_R 162_N 1.44 0.82
193_A 248_H 1.422 0.80
219_L 235_P 1.421 0.80
219_L 232_D 1.402 0.79
44_I 156_Y 1.394 0.79
146_I 189_Q 1.391 0.78
234_T 238_Q 1.385 0.78
51_Y 178_G 1.384 0.78
140_A 185_Q 1.359 0.76
93_E 155_R 1.357 0.76
14_L 17_C 1.321 0.73
63_L 250_R 1.317 0.73
250_R 255_N 1.304 0.72
96_L 108_A 1.301 0.71
10_A 210_H 1.298 0.71
143_Q 150_K 1.291 0.71
149_A 191_I 1.287 0.70
42_P 137_F 1.286 0.70
163_V 225_Y 1.284 0.70
37_A 62_T 1.28 0.70
24_V 27_E 1.248 0.67
103_S 164_V 1.245 0.67
108_A 113_D 1.245 0.67
268_A 272_K 1.239 0.66
225_Y 270_L 1.235 0.66
90_L 194_W 1.231 0.65
153_I 163_V 1.228 0.65
103_S 222_L 1.213 0.64
193_A 273_Y 1.209 0.63
193_A 272_K 1.209 0.63
232_D 235_P 1.207 0.63
63_L 73_L 1.205 0.63
56_F 103_S 1.204 0.63
53_A 179_P 1.197 0.62
70_S 189_Q 1.194 0.62
151_D 155_R 1.187 0.61
216_A 231_P 1.18 0.60
264_A 268_A 1.161 0.59
60_L 187_A 1.16 0.59
3_R 19_G 1.158 0.58
249_F 269_L 1.158 0.58
164_V 268_A 1.157 0.58
135_A 180_L 1.147 0.57
204_L 260_A 1.147 0.57
191_I 218_L 1.133 0.56
225_Y 269_L 1.132 0.56
30_Q 81_Y 1.129 0.55
195_P 265_I 1.122 0.55
57_D 61_A 1.121 0.55
49_I 149_A 1.118 0.54
41_Y 113_D 1.118 0.54
56_F 75_P 1.117 0.54
215_T 235_P 1.116 0.54
139_P 143_Q 1.115 0.54
115_S 120_L 1.113 0.54
250_R 261_E 1.112 0.54
202_F 271_E 1.104 0.53
146_I 150_K 1.096 0.52
128_S 131_V 1.096 0.52
120_L 232_D 1.091 0.52
123_R 135_A 1.087 0.51
250_R 265_I 1.083 0.51
259_D 269_L 1.079 0.50
96_L 266_A 1.076 0.50
44_I 267_E 1.075 0.50
49_I 120_L 1.067 0.49
46_V 169_I 1.066 0.49
218_L 258_A 1.063 0.49
41_Y 96_L 1.059 0.48
191_I 242_I 1.056 0.48
126_Q 135_A 1.056 0.48
192_G 225_Y 1.056 0.48
109_T 133_Y 1.052 0.48
172_Q 240_R 1.048 0.47
95_E 156_Y 1.044 0.47
164_V 236_R 1.044 0.47
107_G 152_I 1.038 0.46
142_I 237_E 1.038 0.46
48_V 236_R 1.034 0.46
262_T 265_I 1.032 0.46
60_L 90_L 1.029 0.45
73_L 90_L 1.024 0.45
214_D 263_Q 1.021 0.44
185_Q 189_Q 1.017 0.44
15_A 24_V 1.006 0.43
121_E 235_P 1.004 0.43
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4bxjA20.89491000.408Contact Map0.624
4bolA20.92751000.41Contact Map0.586
3d2yA10.94571000.42Contact Map0.544
1j3gA10.58331000.67Contact Map0.399
3hmaA20.51811000.719Contact Map0.552
3latA20.64491000.724Contact Map0.551
1yb0A30.51091000.732Contact Map0.558
2f2lA10.54351000.743Contact Map0.535
2f2lX10.52541000.743Contact Map0.565
1yckA10.5581000.746Contact Map0.561

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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