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CUSB - Cation efflux system protein CusB
UniProt: P77239 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG14235
Length: 407 (389)
Sequences: 866
Seq/Len: 2.23

CUSB
Paralog alert: 0.22 [within 20: 0.02] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
114_V 246_V 3.543 1.00
353_K 375_E 3.463 1.00
149_P 209_T 2.849 1.00
355_V 367_L 2.849 1.00
248_A 315_L 2.717 1.00
114_V 244_V 2.637 1.00
102_G 364_V 2.573 1.00
101_R 373_E 2.483 1.00
212_A 238_I 2.407 1.00
268_V 313_A 2.39 1.00
248_A 298_L 2.245 1.00
278_I 298_L 2.23 1.00
274_K 306_A 2.135 1.00
356_A 369_S 2.13 1.00
102_G 326_L 2.117 1.00
182_L 199_I 2.072 0.99
357_V 367_L 2.07 0.99
334_D 339_Q 2.054 0.99
345_D 351_V 2.049 0.99
122_A 214_I 2.008 0.99
359_Q 366_A 1.983 0.99
98_T 376_K 1.954 0.99
47_D 61_S 1.94 0.99
264_F 298_L 1.905 0.99
117_N 243_P 1.891 0.99
266_L 300_V 1.891 0.99
59_G 68_L 1.869 0.99
117_N 120_Q 1.862 0.99
50_Y 61_S 1.853 0.98
61_S 64_M 1.82 0.98
61_S 68_L 1.814 0.98
62_P 68_L 1.762 0.98
50_Y 53_T 1.737 0.97
47_D 50_Y 1.715 0.97
136_Y 149_P 1.714 0.97
357_V 365_T 1.697 0.97
343_T 375_E 1.672 0.96
329_S 365_T 1.645 0.96
255_A 294_L 1.643 0.96
263_Q 279_R 1.633 0.96
263_Q 277_T 1.633 0.96
241_M 309_P 1.595 0.95
326_L 364_V 1.592 0.95
191_P 194_D 1.573 0.95
264_F 317_L 1.52 0.93
327_I 365_T 1.519 0.93
47_D 64_M 1.519 0.93
124_V 212_A 1.514 0.93
53_T 61_S 1.51 0.93
64_M 72_Y 1.51 0.93
124_V 238_I 1.489 0.92
59_G 62_P 1.475 0.92
120_Q 240_G 1.465 0.91
245_W 297_R 1.453 0.91
344_V 376_K 1.436 0.90
327_I 367_L 1.396 0.89
146_K 259_K 1.395 0.88
264_F 278_I 1.388 0.88
137_P 218_I 1.381 0.88
281_W 299_E 1.38 0.88
272_P 276_L 1.371 0.87
50_Y 64_M 1.363 0.87
127_R 189_G 1.357 0.87
47_D 68_L 1.357 0.87
220_A 237_K 1.351 0.86
264_F 315_L 1.348 0.86
338_E 354_R 1.332 0.85
47_D 53_T 1.326 0.85
258_V 280_K 1.324 0.85
47_D 63_F 1.317 0.84
186_R 192_E 1.313 0.84
250_I 296_L 1.312 0.84
55_F 61_S 1.302 0.83
45_W 61_S 1.279 0.82
46_Y 50_Y 1.271 0.81
46_Y 64_M 1.269 0.81
329_S 363_G 1.266 0.81
96_T 379_S 1.265 0.81
44_F 64_M 1.264 0.81
89_T 350_F 1.254 0.80
137_P 148_T 1.249 0.80
134_K 152_D 1.248 0.80
248_A 264_F 1.243 0.79
165_Y 198_L 1.229 0.78
61_S 72_Y 1.228 0.78
327_I 355_V 1.225 0.78
47_D 52_N 1.21 0.77
252_E 311_M 1.209 0.77
46_Y 55_F 1.207 0.76
358_F 366_A 1.205 0.76
212_A 215_D 1.204 0.76
50_Y 55_F 1.201 0.76
44_F 47_D 1.199 0.76
341_V 371_L 1.192 0.75
145_Q 215_D 1.186 0.75
85_D 88_Q 1.186 0.75
50_Y 59_G 1.179 0.74
47_D 55_F 1.178 0.74
307_L 311_M 1.174 0.74
246_V 266_L 1.174 0.74
112_A 148_T 1.173 0.73
251_P 254_I 1.173 0.73
50_Y 68_L 1.171 0.73
118_E 140_V 1.171 0.73
129_A 230_A 1.169 0.73
55_F 65_D 1.169 0.73
45_W 54_R 1.163 0.73
95_K 330_Q 1.162 0.72
43_L 64_M 1.161 0.72
52_N 61_S 1.159 0.72
265_T 275_T 1.157 0.72
146_K 215_D 1.151 0.71
395_L 398_M 1.147 0.71
46_Y 52_N 1.145 0.71
151_L 210_L 1.143 0.71
113_N 249_A 1.142 0.71
129_A 231_K 1.131 0.70
244_V 309_P 1.123 0.69
153_L 229_I 1.121 0.69
46_Y 71_K 1.12 0.68
60_K 64_M 1.118 0.68
99_V 374_G 1.117 0.68
55_F 62_P 1.11 0.67
40_R 74_D 1.108 0.67
246_V 315_L 1.107 0.67
327_I 341_V 1.105 0.67
50_Y 62_P 1.096 0.66
183_E 186_R 1.095 0.66
46_Y 61_S 1.095 0.66
196_R 199_I 1.089 0.65
47_D 59_G 1.089 0.65
272_P 311_M 1.089 0.65
139_T 142_D 1.088 0.65
372_A 375_E 1.087 0.65
51_P 59_G 1.084 0.65
265_T 277_T 1.083 0.65
244_V 300_V 1.082 0.65
122_A 238_I 1.08 0.64
134_K 225_A 1.076 0.64
219_T 239_Q 1.069 0.63
383_F 386_D 1.065 0.63
343_T 353_K 1.063 0.63
117_N 245_W 1.063 0.63
50_Y 63_F 1.062 0.62
128_A 153_L 1.06 0.62
49_M 66_M 1.056 0.62
15_G 38_A 1.053 0.61
109_S 314_W 1.052 0.61
381_G 389_A 1.05 0.61
185_L 188_A 1.047 0.61
47_D 62_P 1.047 0.61
46_Y 53_T 1.046 0.61
109_S 316_Q 1.043 0.60
112_A 313_A 1.039 0.60
54_R 57_K 1.036 0.60
121_Y 239_Q 1.034 0.59
330_Q 363_G 1.033 0.59
52_N 59_G 1.033 0.59
384_L 388_E 1.032 0.59
136_Y 209_T 1.032 0.59
276_L 311_M 1.029 0.59
45_W 64_M 1.027 0.59
43_L 47_D 1.026 0.59
201_T 350_F 1.026 0.59
96_T 376_K 1.025 0.58
280_K 344_V 1.025 0.58
46_Y 62_P 1.024 0.58
27_K 30_P 1.02 0.58
54_R 61_S 1.015 0.57
110_F 335_T 1.015 0.57
169_R 178_T 1.012 0.57
293_T 297_R 1.011 0.57
14_G 33_E 1.011 0.57
17_I 26_A 1.008 0.56
33_E 36_S 1.008 0.56
14_G 28_A 1.006 0.56
279_R 304_D 1.005 0.56
54_R 68_L 1.002 0.56
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3ne5B20.99261000.242Contact Map0.756
3lnnA20.79851000.542Contact Map0.772
1vf7A130.79121000.578Contact Map0.758
4dk0A10.74451000.586Contact Map0.579
3fppA20.72731000.589Contact Map0.757
4kkuA40.70761000.59Contact Map0.684
4l8jA10.73461000.596Contact Map0.459
2f1mA40.57991000.683Contact Map0.632
2k32A10.282699.80.839Contact Map0.81
2dneA10.250699.70.852Contact Map0.32

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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