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CAS1 - CRISPR-associated endonuclease Cas1
UniProt: Q46896 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13114
Length: 305 (274)
Sequences: 310
Seq/Len: 1.13

CAS1
Paralog alert: 0.03 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
65_V 75_L 3.269 1.00
223_I 267_L 2.739 1.00
147_A 150_A 2.15 0.98
20_L 34_L 2.145 0.98
119_M 271_V 2.04 0.97
20_L 25_I 1.892 0.95
11_L 50_A 1.878 0.94
191_T 264_L 1.836 0.93
121_E 127_P 1.789 0.92
122_L 222_I 1.677 0.89
226_D 263_K 1.676 0.89
118_K 270_D 1.664 0.88
231_K 235_I 1.592 0.85
162_G 238_R 1.557 0.83
10_P 13_D 1.55 0.83
111_L 277_I 1.516 0.81
184_T 229_V 1.514 0.81
155_Q 234_E 1.508 0.80
28_I 37_K 1.501 0.80
16_S 50_A 1.484 0.79
195_I 268_I 1.476 0.78
222_I 264_L 1.474 0.78
235_I 250_A 1.442 0.76
251_C 255_F 1.423 0.75
228_V 251_C 1.416 0.74
63_A 66_R 1.339 0.68
66_R 70_Q 1.331 0.67
110_D 130_A 1.314 0.66
184_T 247_V 1.304 0.65
89_G 165_Y 1.303 0.65
195_I 213_L 1.302 0.65
17_M 22_Y 1.292 0.64
106_A 134_V 1.288 0.64
99_L 203_A 1.283 0.63
52_I 257_S 1.281 0.63
25_I 48_S 1.279 0.63
18_I 22_Y 1.275 0.63
223_I 264_L 1.257 0.61
195_I 216_V 1.251 0.60
88_S 195_I 1.248 0.60
245_R 249_L 1.247 0.60
51_C 264_L 1.243 0.60
52_I 134_V 1.235 0.59
24_Q 59_R 1.232 0.59
104_K 118_K 1.23 0.58
112_R 137_L 1.224 0.58
20_L 180_I 1.19 0.55
123_R 163_R 1.189 0.55
13_D 66_R 1.184 0.54
222_I 267_L 1.181 0.54
58_T 174_D 1.18 0.54
236_A 243_P 1.177 0.53
139_G 223_I 1.175 0.53
264_L 267_L 1.174 0.53
132_R 140_I 1.173 0.53
53_M 71_V 1.17 0.53
109_E 244_D 1.17 0.53
20_L 23_G 1.166 0.52
200_Y 212_P 1.164 0.52
268_I 272_L 1.161 0.52
127_P 130_A 1.157 0.52
111_L 185_S 1.155 0.51
158_V 176_I 1.153 0.51
19_F 55_E 1.152 0.51
227_T 258_S 1.142 0.50
204_I 207_V 1.139 0.50
121_E 153_A 1.138 0.50
161_N 174_D 1.135 0.49
32_F 47_G 1.131 0.49
14_R 67_L 1.125 0.48
240_P 246_E 1.123 0.48
50_A 58_T 1.122 0.48
65_V 171_E 1.119 0.48
23_G 30_G 1.117 0.48
100_L 116_V 1.113 0.47
92_G 211_K 1.113 0.47
139_G 149_Y 1.112 0.47
181_S 214_S 1.111 0.47
29_D 268_I 1.11 0.47
49_V 262_A 1.104 0.46
162_G 176_I 1.102 0.46
11_L 88_S 1.101 0.46
16_S 58_T 1.099 0.46
17_M 155_Q 1.097 0.46
176_I 236_A 1.096 0.46
52_I 86_Y 1.095 0.46
66_R 69_A 1.094 0.46
60_V 65_V 1.087 0.45
91_P 197_A 1.087 0.45
122_L 267_L 1.086 0.45
69_A 267_L 1.085 0.45
53_M 198_A 1.084 0.45
119_M 219_I 1.08 0.44
14_R 30_G 1.08 0.44
159_T 172_K 1.079 0.44
76_V 162_G 1.075 0.44
26_D 200_Y 1.074 0.44
22_Y 86_Y 1.074 0.44
26_D 33_V 1.071 0.43
99_L 201_A 1.07 0.43
85_V 213_L 1.07 0.43
136_Q 181_S 1.07 0.43
83_V 253_D 1.068 0.43
24_Q 63_A 1.062 0.42
183_A 247_V 1.061 0.42
133_S 136_Q 1.06 0.42
124_F 226_D 1.058 0.42
115_V 206_F 1.057 0.42
58_T 61_S 1.056 0.42
52_I 244_D 1.054 0.42
176_I 228_V 1.052 0.42
27_V 67_L 1.052 0.42
231_K 254_I 1.051 0.41
11_L 34_L 1.05 0.41
148_T 151_L 1.048 0.41
128_A 153_A 1.045 0.41
152_L 155_Q 1.043 0.41
106_A 187_L 1.043 0.41
86_Y 139_G 1.041 0.41
49_V 276_E 1.041 0.41
61_S 156_Y 1.038 0.40
50_A 203_A 1.036 0.40
128_A 154_K 1.033 0.40
89_G 167_P 1.031 0.40
6_L 71_V 1.028 0.39
96_S 120_F 1.027 0.39
68_A 94_A 1.027 0.39
82_G 230_P 1.024 0.39
25_I 58_T 1.022 0.39
87_A 263_K 1.021 0.39
185_S 277_I 1.021 0.39
52_I 245_R 1.018 0.38
56_P 86_Y 1.016 0.38
153_A 172_K 1.016 0.38
74_L 271_V 1.016 0.38
178_Q 260_T 1.015 0.38
69_A 270_D 1.013 0.38
69_A 108_D 1.011 0.38
190_V 262_A 1.009 0.38
110_D 175_T 1.009 0.38
147_A 245_R 1.007 0.37
73_T 264_L 1.006 0.37
144_R 165_Y 1.006 0.37
147_A 173_G 1.005 0.37
69_A 110_D 1.004 0.37
34_L 63_A 1.004 0.37
138_R 143_S 1.003 0.37
21_Q 42_T 1.003 0.37
73_T 98_K 1.003 0.37
27_V 30_G 1.003 0.37
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3nkdA211000.432Contact Map0.404
3godA40.89841000.516Contact Map0.365
3nkeA30.65571000.609Contact Map0.486
3lfxA80.86231000.658Contact Map0.295
2yzsA20.87871000.664Contact Map0.278
3pv9D20.87871000.665Contact Map0.286
2p17A10.311510.50.979Contact Map0.008
4hlnA10.26569.70.98Contact Map
2apnA10.18034.20.983Contact Map0
3hvmA10.28853.90.983Contact Map0.325

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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