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CAS6 - CRISPR system Cascade subunit CasE
UniProt: Q46897 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13115
Length: 199 (199)
Sequences: 277
Seq/Len: 1.39

CAS6
Paralog alert: 0.01 [within 20: 0.00] - ratio of genomes with paralogs
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
113_R 185_P 2.796 1.00
17_Y 189_M 2.637 1.00
78_L 169_I 2.568 1.00
3_L 55_Q 2.295 0.99
27_F 56_S 2.188 0.99
24_W 37_F 2.119 0.99
87_R 164_E 1.988 0.98
133_A 167_L 1.754 0.94
89_R 162_C 1.733 0.93
175_L 196_L 1.718 0.93
55_Q 74_V 1.696 0.92
178_L 183_I 1.684 0.92
183_I 194_L 1.631 0.90
90_A 163_F 1.617 0.90
174_A 177_D 1.578 0.88
104_D 108_N 1.565 0.88
84_L 167_L 1.52 0.86
100_Q 103_L 1.514 0.85
167_L 175_L 1.479 0.83
23_L 161_V 1.467 0.83
88_L 126_L 1.458 0.82
116_L 129_K 1.452 0.82
123_I 139_V 1.448 0.82
186_A 191_C 1.446 0.82
55_Q 195_S 1.444 0.81
179_V 196_L 1.435 0.81
161_V 167_L 1.386 0.78
83_P 168_T 1.383 0.77
37_F 189_M 1.381 0.77
163_F 185_P 1.376 0.77
133_A 175_L 1.369 0.76
166_V 170_N 1.349 0.75
24_W 189_M 1.31 0.72
99_N 102_R 1.306 0.71
22_G 186_A 1.302 0.71
162_C 195_S 1.283 0.70
26_L 62_S 1.282 0.69
86_F 126_L 1.256 0.67
90_A 126_L 1.249 0.67
74_V 132_N 1.242 0.66
134_A 168_T 1.229 0.65
144_E 162_C 1.2 0.62
185_P 194_L 1.197 0.62
39_F 122_Q 1.194 0.61
1_M 56_S 1.194 0.61
116_L 121_E 1.194 0.61
62_S 71_T 1.188 0.61
18_Q 151_G 1.186 0.61
22_G 185_P 1.184 0.60
130_L 133_A 1.176 0.60
124_A 128_R 1.175 0.60
95_T 113_R 1.171 0.59
23_L 189_M 1.162 0.58
38_L 55_Q 1.16 0.58
40_H 195_S 1.159 0.58
21_Q 55_Q 1.157 0.58
85_Y 194_L 1.149 0.57
90_A 125_W 1.147 0.57
85_Y 197_A 1.137 0.56
15_D 194_L 1.137 0.56
175_L 183_I 1.12 0.54
71_T 93_I 1.115 0.54
5_K 158_I 1.11 0.53
93_I 185_P 1.105 0.53
53_L 114_V 1.097 0.52
37_F 191_C 1.094 0.51
14_R 187_K 1.094 0.51
93_I 181_Q 1.092 0.51
131_G 198_P 1.092 0.51
87_R 195_S 1.089 0.51
80_V 172_A 1.089 0.51
4_S 86_F 1.088 0.51
8_I 59_M 1.087 0.51
36_D 58_Q 1.085 0.51
5_K 70_K 1.062 0.48
118_K 121_E 1.061 0.48
95_T 185_P 1.061 0.48
84_L 196_L 1.053 0.47
29_N 72_K 1.052 0.47
122_Q 161_V 1.052 0.47
181_Q 199_L 1.051 0.47
86_F 167_L 1.049 0.47
160_T 191_C 1.047 0.47
23_L 116_L 1.046 0.47
17_Y 37_F 1.046 0.47
169_I 175_L 1.041 0.46
130_L 175_L 1.038 0.46
39_F 167_L 1.027 0.45
121_E 191_C 1.024 0.44
32_D 139_V 1.024 0.44
55_Q 159_Q 1.017 0.44
154_K 183_I 1.011 0.43
22_G 149_F 1.011 0.43
119_E 122_Q 1.007 0.43
65_V 189_M 1.007 0.43
34_A 71_T 1.007 0.43
172_A 175_L 1.003 0.42
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4dzdA111000.079Contact Map0.512
3qrpA10.93471000.148Contact Map0.508
2y8yA10.93471000.149Contact Map0.513
4c97A20.894525.70.949Contact Map0.304
1vbiA10.42218.70.959Contact Map0.232
3i4hX10.94478.60.959Contact Map0.344
1nxuA20.42718.10.959Contact Map0.214
1rfmA80.42717.70.96Contact Map0.207
4h8aA20.43726.10.962Contact Map0.243
2kegA10.12064.50.964Contact Map0

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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