GREMLIN.BAKERLAB.org

Archive: 2012 - 2013
Cellulase - Cellulase (glycosyl hydrolase family 5)
Pfam: PF00150 (v27) Consensus Sequence
Download Alignment
(Note: includes all positions in family; we filtered this alignment to remove sites that had > 75% gaps before running GREMLIN. )
Clan: CL0058
Length: 281
Sequences: 5363
Seq/Len: 19.09
HH_delta: 0.265 (20Jul13)
GREMLIN Results:
Residue pairs sorted by strength in covariance:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] gremlin predictions, sequence seperation > 3. The i and j are positions as given in the consensus sequence. Show Scaled Distribution

ij Raw Score Scaled Score
28_D74_Y1.028684.723
65_D115_R0.958564.401
37_T273_I0.733613.369
65_D116_Y0.687093.155
27_F71_A0.580832.667
24_E74_Y0.569852.617
153_Q157_D0.56012.572
24_E70_A0.540452.482
24_E73_A0.513722.359
28_D32_A0.505892.323
71_A78_V0.497082.282
110_R158_A0.457072.099
70_A73_A0.456382.096
31_K74_Y0.440892.024
110_R161_A0.424381.949
106_K158_A0.423951.947
125_W163_D0.421751.937
158_A161_A0.418951.924
102_Q154_R0.415521.908
113_A162_A0.413971.901
79_I124_G0.413851.900
69_D73_A0.410481.885
30_L35_F0.402531.848
27_F74_Y0.402051.846
163_D166_H0.401531.844
156_I168_I0.393741.808
230_N234_K0.386911.777
114_K117_K0.380711.748
65_D69_D0.377851.735
244_F272_W0.376681.730
126_E171_G0.375311.723
257_Y261_W0.375051.722
106_K154_R0.37181.707
126_E197_S0.37061.702
169_I195_V0.36971.698
110_R114_K0.362131.663
42_V80_L0.352061.617
103_A107_S0.35091.611
160_R168_I0.348911.602
157_D161_A0.345591.587
158_A162_A0.343171.576
71_A76_I0.338321.553
24_E28_D0.338221.553
226_R230_N0.332481.527
72_Q119_N0.330221.516
150_D154_R0.328931.510
264_Y268_N0.327251.503
69_D119_N0.327151.502
259_D263_D0.324421.490
107_S110_R0.322151.479
148_W152_Y0.321781.478
70_A74_Y0.317621.458
197_S242_G0.317621.458
114_K162_A0.311241.429
30_L76_I0.309221.420
258_A261_W0.29671.362
69_D72_Q0.292491.343
125_W166_H0.287061.318
124_G169_I0.284551.307
29_Q32_A0.282421.297
85_A128_W0.277461.274
127_L152_Y0.276571.270
110_R162_A0.275851.267
226_R268_N0.275591.265
167_L195_V0.27441.260
127_L159_I0.272321.250
106_K110_R0.27181.248
260_A263_D0.271731.248
156_I160_R0.271561.247
113_A125_W0.269871.239
25_A28_D0.269521.238
113_A159_I0.267381.228
37_T77_Y0.262111.204
39_R129_N0.26211.203
159_I168_I0.260761.197
129_N243_E0.259591.192
72_Q121_P0.259461.191
31_K75_G0.256211.176
40_I78_V0.255361.173
79_I273_I0.254371.168
111_A114_K0.252641.160
79_I169_I0.252071.157
68_V78_V0.251981.157
61_L115_R0.251551.155
27_F38_V0.25141.154
223_S227_A0.250021.148
68_V116_Y0.244641.123
240_V273_I0.24421.121
222_E226_R0.243361.117
36_N77_Y0.240881.106
40_I64_L0.240131.103
25_A29_Q0.239451.099
156_I192_N0.238381.095
102_Q106_K0.237281.090
201_Y243_E0.235731.082
199_H242_G0.23551.081
127_L155_A0.23241.067
271_G275_W0.23221.066
150_D153_Q0.232081.066
27_F67_I0.228741.050
117_K163_D0.227931.047
130_E243_E0.227791.046
255_T259_D0.227711.046
38_V78_V0.226331.039
128_W131_P0.225871.037
24_E66_R0.225811.037
107_S111_A0.224631.031
22_I26_D0.223041.024
126_E199_H0.219451.008
262_L266_E0.218311.002
40_I71_A0.217120.997
231_W235_N0.216350.993
155_A159_I0.215860.991
97_N100_T0.215840.991
103_A106_K0.214420.985
39_R199_H0.211210.970
109_W113_A0.210690.967
125_W159_I0.210580.967
155_A158_A0.208630.958
153_Q192_N0.205550.944
121_P124_G0.204830.941
225_F228_A0.204740.940
77_Y123_V0.20380.936
224_S228_A0.203710.935
154_R158_A0.203210.933
30_L38_V0.202930.932
126_E242_G0.201020.923
263_D267_Q0.198530.912
256_D260_A0.198310.911
160_R194_D0.198020.909
31_K76_I0.197930.909
29_Q33_L0.197760.908
171_G199_H0.197340.906
66_R70_A0.197060.905
50_P53_G0.195020.895
161_A164_P0.194120.891
109_W125_W0.193690.889
132_N173_G0.193170.887
257_Y260_A0.192790.885
152_Y170_V0.192060.882
149_Q153_Q0.19080.876
229_L233_K0.190.872
80_L83_H0.189490.870
265_L268_N0.187770.862
62_A66_R0.187270.860
198_F239_V0.186540.857
263_D266_E0.186060.854
264_Y267_Q0.185650.852
38_V76_I0.184920.849
167_L193_N0.183750.844
262_L272_W0.183430.842
27_F31_K0.18270.839
123_V167_L0.182080.836
157_D192_N0.181860.835
223_S226_R0.181710.834
128_W148_W0.181530.834
80_L116_Y0.181010.831
61_L111_A0.180040.827
112_L116_Y0.179680.825
83_H275_W0.179420.824
152_Y193_N0.178880.821
154_R157_D0.178280.819
59_T62_A0.178180.818
241_V265_L0.177810.816
28_D31_K0.177660.816
40_I67_I0.177480.815
44_W47_Y0.177350.814
62_A65_D0.176920.812
44_W48_Q0.176750.812
90_N94_G0.175860.808
62_A115_R0.175510.806
178_D181_G0.175470.806
27_F76_I0.1750.804
232_A239_V0.174750.802
39_R81_D0.174640.802
115_R118_D0.174530.801
90_N93_D0.17450.801
57_D60_Y0.1740.799
72_Q120_P0.173680.797
104_W108_F0.173610.797
246_W258_A0.173490.797
159_I164_P0.173120.795
63_R66_R0.171640.788
64_L80_L0.170670.784
108_F112_L0.169380.778
66_R69_D0.169140.777
26_D30_L0.169070.776
160_R193_N0.168690.775
81_D126_E0.168650.774
203_P207_S0.168180.772
28_D70_A0.167390.769
90_N95_Y0.166320.764
109_W159_I0.165840.761
147_D150_D0.165680.761
61_L65_D0.165390.759
113_A158_A0.164860.757
169_I197_S0.164410.755
202_D246_W0.164160.754
37_T240_V0.164020.753
68_V119_N0.163820.752
198_F241_V0.163810.752
122_V125_W0.163410.750
124_G167_L0.162870.748
200_F241_V0.162170.745
102_Q105_F0.161720.743
202_D244_F0.161310.741
129_N199_H0.160870.739
82_L109_W0.160490.737
266_E272_W0.159640.733
41_P242_G0.159520.732
58_E62_A0.159460.732
100_T103_A0.159210.731
203_P206_F0.158320.727
38_V71_A0.15830.727
24_E27_F0.15820.726
22_I27_F0.158050.726
160_R163_D0.158040.726
161_A165_N0.15760.724
262_L265_L0.157510.723
127_L168_I0.157290.722
155_A168_I0.157240.722
41_P83_H0.157120.721
194_D237_K0.156280.718
44_W86_P0.155920.716
130_E199_H0.155510.714
82_L112_L0.154550.710
244_F262_L0.154360.709
172_G197_S0.153310.704
37_T79_I0.152230.699
99_D103_A0.150850.693
41_P84_N0.150750.692
27_F70_A0.150550.691
72_Q123_V0.150320.690
46_A60_Y0.150190.690
131_P199_H0.149550.687
103_A154_R0.148940.684
43_G84_N0.14760.678
240_V271_G0.147120.676
106_K155_A0.147110.675
227_A230_N0.146990.675
118_D165_N0.146430.672
65_D119_N0.146270.672
171_G197_S0.144450.663
44_W50_P0.144280.662
23_T26_D0.143520.659
77_Y124_G0.143380.658
230_N233_K0.142920.656
85_A105_F0.142320.653
23_T67_I0.141270.649
151_W171_G0.141050.648
42_V195_V0.140990.647
200_F225_F0.140910.647
159_I162_A0.140460.645
222_E225_F0.140270.644
88_W105_F0.139390.640
59_T63_R0.139350.640
225_F241_V0.13880.637
91_G94_G0.138610.636
83_H155_A0.137580.632
151_W155_A0.137330.631
38_V67_I0.13720.630
84_N105_F0.13690.629
124_G195_V0.136790.628
226_R229_L0.135990.624
61_L112_L0.135980.624
146_A150_D0.135830.624
63_R67_I0.135390.622
265_L270_I0.135320.621
169_I240_V0.135280.621
175_W178_D0.135050.620
153_Q168_I0.134880.619
110_R113_A0.134760.619
41_P81_D0.134630.618
68_V72_Q0.134430.617
225_F229_L0.134230.616
48_Q56_Y0.134210.616
224_S227_A0.133640.614
71_A82_L0.132480.608
126_E169_I0.132240.607
78_V122_V0.132050.606
42_V64_L0.131890.606
91_G100_T0.131750.605
40_I80_L0.131420.603
48_Q104_W0.13130.603
265_L269_G0.130870.601
183_A186_N0.130650.600
129_N275_W0.130620.600
157_D267_Q0.130590.600
35_F274_Y0.130560.599
26_D29_Q0.130470.599
65_D68_V0.129840.596
68_V122_V0.129720.596
109_W156_I0.129340.594
72_Q75_G0.129270.594
195_V240_V0.12920.593
166_H169_I0.129010.592
84_N128_W0.128720.591
199_H243_E0.128630.591
101_A104_W0.12860.590
201_Y245_G0.128480.590
72_Q122_V0.128140.588
115_R119_N0.128090.588
106_K157_D0.127720.586
128_W132_N0.127540.586
100_T104_W0.127420.585
93_D97_N0.127190.584
84_N151_W0.127160.584
125_W156_I0.127050.583
37_T246_W0.1270.583
68_V80_L0.126430.581
31_K34_G0.126420.580
256_D261_W0.126380.580
256_D259_D0.126370.580
258_A265_L0.126250.580
123_V166_H0.125950.578
251_G259_D0.125470.576
258_A274_Y0.12540.576
148_W151_W0.125230.575
225_F265_L0.125190.575
98_N102_Q0.125020.574
41_P275_W0.123920.569
156_I159_I0.123530.567
111_A115_R0.123410.567
233_K236_G0.123220.566
272_W275_W0.122820.564
153_Q191_D0.122780.564
96_G99_D0.122740.564
180_D228_A0.122720.563
56_Y59_T0.122660.563
117_K164_P0.122640.563
156_I193_N0.122550.563
271_G274_Y0.121650.559
260_A272_W0.121210.557
Legend: The value of the raw score is the function of the learning procedure, L2 normalization and APC (entropic) correction. These are to be used for relative ranking only.
HHsearch Results:
Top (length/2) GREMLIN results overlayed on top 10 PDB hits:

Legend: The grey circles underneath are pdb residue contacts (min distance < 5 Angstroms). The coloring of these circles is based on HHsearch results which uses the overall probability, per-site alignment prob and agreement of top hits weighted by HHsearch score (Note we only consider monomeric contacts, there might be homo-oligomeric contacts in the pdb that are not shown.)
PDB Cov Prob HH_delta
1eceA0.97511000.265
4htyA0.9111000.265
2osxA0.97861000.268
3qhoA0.99291000.27
3aofA0.94661000.275
3icgA0.98931000.276
1edgA0.99641000.277
3ndzA0.98931000.279
3pztA0.86121000.287
7a3hA0.86481000.287

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