GREMLIN.BAKERLAB.org

Archive: 2012 - 2013
Peptidase_M16 - Insulinase (Peptidase family M16)
Pfam: PF00675 (v27) Consensus Sequence
Download Alignment
(Note: includes all positions in family; we filtered this alignment to remove sites that had > 75% gaps before running GREMLIN. )
Clan: CL0094
Length: 149
Sequences: 6154
Seq/Len: 41.3
HH_delta: 0.052 (20Jul13)
GREMLIN Results:
Residue pairs sorted by strength in covariance:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] gremlin predictions, sequence seperation > 3. The i and j are positions as given in the consensus sequence. Show Scaled Distribution

ij Raw Score Scaled Score
102_E106_E1.137353.424
46_K97_N1.094463.295
49_S52_E1.036383.120
67_S74_S0.984492.964
79_V87_A0.912652.748
106_E109_R0.800722.411
130_K133_S0.778712.344
47_K93_D0.772972.327
47_K89_E0.732662.206
48_Y93_D0.710192.138
93_D97_N0.703872.119
60_L86_K0.691062.081
27_P30_K0.649821.956
56_E86_K0.643751.938
48_Y56_E0.641711.932
101_D104_E0.630341.898
45_S93_D0.627571.889
77_A87_A0.610731.839
36_L105_F0.605341.823
56_E59_S0.579911.746
70_R132_H0.566831.707
109_R113_L0.561851.692
46_K99_S0.559011.683
57_L62_A0.548941.653
71_D141_Y0.536991.617
60_L83_D0.532231.602
14_S78_S0.511331.539
43_R108_E0.499721.505
137_R142_G0.494341.488
13_V81_S0.474361.428
37_L100_F0.468251.410
47_K97_N0.464081.397
18_V74_S0.451051.358
83_D86_K0.443821.336
57_L64_F0.440821.327
92_A96_F0.430431.296
19_F95_L0.430391.296
56_E60_L0.430041.295
35_H38_E0.427011.286
67_S72_S0.426351.284
35_H39_H0.416061.253
28_P143_N0.409121.232
86_K89_E0.408191.229
41_L98_P0.406781.225
110_E113_L0.401251.208
114_Q117_E0.401221.208
38_E68_T0.397671.197
34_A37_L0.396581.194
128_F132_H0.391881.180
52_E56_E0.386221.163
113_L117_E0.38191.150
16_S74_S0.380531.146
36_L109_R0.379811.144
40_L105_F0.379591.143
130_K134_A0.375741.131
35_H115_E0.374971.129
54_Q64_F0.368731.110
35_H112_I0.36871.110
36_L112_I0.367911.108
53_L94_M0.36731.106
30_K33_L0.367281.106
17_L95_L0.363061.093
134_A137_R0.361921.090
122_N125_E0.358241.079
104_E107_R0.355711.071
138_G142_G0.353871.065
39_H115_E0.351251.058
53_L90_L0.34741.046
55_E58_E0.346761.044
127_A131_L0.344751.038
15_V77_A0.343641.035
48_Y89_E0.339021.021
112_I115_E0.336311.013
85_E89_E0.332631.001
48_Y90_L0.332251.000
11_P81_S0.328510.989
66_A75_Y0.328460.989
39_H65_N0.326670.984
126_L130_K0.325640.980
23_S145_L0.325280.979
53_L57_L0.324240.976
39_H68_T0.323870.975
13_V84_L0.323440.974
17_L91_L0.321640.968
40_L43_R0.321260.967
77_A91_L0.321210.967
91_L94_M0.318140.958
39_H112_I0.316450.953
80_L83_D0.31610.952
26_E32_G0.315850.951
38_E66_A0.313090.943
108_E111_Q0.312120.940
108_E112_I0.310910.936
114_Q118_E0.309930.933
16_S76_S0.305590.920
21_A73_T0.304780.918
100_F105_F0.304690.917
56_E90_L0.304380.916
31_P143_N0.300570.905
38_E42_F0.2970.894
103_E107_R0.292780.881
12_V78_S0.292680.881
105_F109_R0.29180.879
39_H108_E0.290.873
23_S70_R0.288830.870
21_A25_Y0.287650.866
38_E73_T0.286920.864
33_L37_L0.284410.856
51_D55_E0.284110.855
43_R104_E0.27940.841
119_I125_E0.277620.836
106_E110_E0.275160.828
24_R71_D0.274810.827
39_H42_F0.269790.812
41_L75_Y0.268180.807
4_L7_D0.268050.807
67_S76_S0.267360.805
62_A79_V0.267350.805
111_Q115_E0.266990.804
117_E120_K0.26670.803
41_L94_M0.264170.795
35_H68_T0.260870.785
30_K34_A0.257430.775
139_H142_G0.255030.768
15_V91_L0.249820.752
119_I129_E0.249530.751
86_K90_L0.248990.750
116_I129_E0.246580.742
32_G35_H0.246110.741
14_S76_S0.243880.734
43_R111_Q0.243320.733
55_E59_S0.243180.732
23_S26_E0.241910.728
15_V87_A0.241770.728
45_S48_Y0.241730.728
52_E55_E0.241320.727
26_E30_K0.239440.721
93_D96_F0.237610.715
136_F141_Y0.237010.714
133_S143_N0.236710.713
43_R107_R0.234820.707
42_F66_A0.23320.702
125_E129_E0.233130.702
50_S104_E0.23270.701
120_K129_E0.231510.697
68_T73_T0.23080.695
111_Q114_Q0.230640.694
70_R145_L0.230370.694
6_E9_G0.229540.691
44_G101_D0.22850.688
35_H111_Q0.228280.687
89_E92_A0.227580.685
66_A94_M0.226470.682
103_E106_E0.225150.678
62_A87_A0.224610.676
116_I120_K0.224370.676
44_G104_E0.223930.674
131_L134_A0.221980.668
32_G38_E0.220470.664
17_L92_A0.220430.664
19_F73_T0.219590.661
7_D10_S0.217120.654
26_E31_P0.216850.653
60_L90_L0.216720.652
69_S72_S0.212490.640
35_H75_Y0.211910.638
32_G39_H0.211010.635
38_E112_I0.210630.634
22_G34_A0.21020.633
45_S97_N0.208440.628
38_E115_E0.207050.623
126_L129_E0.206660.622
8_P11_P0.206260.621
20_K25_Y0.205980.620
40_L100_F0.205940.620
116_I121_E0.205660.619
15_V88_L0.205050.617
42_F115_E0.20130.606
66_A73_T0.20050.604
45_S99_S0.194590.586
7_D11_P0.194210.585
48_Y53_L0.1920.578
69_S74_S0.19090.575
28_P138_G0.190480.573
128_F131_L0.190220.573
21_A34_A0.189830.572
35_H66_A0.189720.571
39_H111_Q0.189420.570
118_E121_E0.188560.568
66_A115_E0.187670.565
34_A39_H0.187260.564
28_P31_P0.185890.560
124_Q128_F0.185080.557
37_L41_L0.184650.556
18_V72_S0.184440.555
42_F65_N0.183810.553
84_L87_A0.183780.553
5_V10_S0.183270.552
92_A95_L0.183130.551
23_S32_G0.183030.551
13_V79_V0.182510.549
102_E109_R0.181820.547
37_L73_T0.180940.545
19_F75_Y0.180660.544
110_E114_Q0.179050.539
65_N115_E0.177850.535
75_Y115_E0.176310.531
129_E144_P0.176240.531
6_E11_P0.17570.529
15_V79_V0.174950.527
47_K99_S0.17370.523
35_H42_F0.173420.522
131_L135_A0.172180.518
42_F68_T0.172110.518
39_H75_Y0.17140.516
41_L95_L0.171390.516
Legend: The value of the raw score is the function of the learning procedure, L2 normalization and APC (entropic) correction. These are to be used for relative ranking only.
HHsearch Results:
Top (length/2) GREMLIN results overlayed on top 10 PDB hits:

Legend: The grey circles underneath are pdb residue contacts (min distance < 5 Angstroms). The coloring of these circles is based on HHsearch results which uses the overall probability, per-site alignment prob and agreement of top hits weighted by HHsearch score (Note we only consider monomeric contacts, there might be homo-oligomeric contacts in the pdb that are not shown.)
PDB Cov Prob HH_delta
3eoqA0.993399.90.052
1q2lA199.90.054
3amiA199.90.065
3hdiA199.90.068
3cwwA199.90.086
1hr6B199.90.102
1pp9A0.993399.90.104
1hr6A0.993399.90.11
3gwbA0.993399.80.141
1pp9B0.986699.80.155

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