GREMLIN.BAKERLAB.org

Archive: 2012 - 2013
Steroid_dh - 3-oxo-5-alpha-steroid 4-dehydrogenase
Pfam: PF02544 (v27) Consensus Sequence
Download Alignment
(Note: includes all positions in family; we filtered this alignment to remove sites that had > 75% gaps before running GREMLIN. )
Clan: CL0115
Length: 150
Sequences: 820
Seq/Len: 5.47
HH_delta: 0.647 (20Jul13)
GREMLIN Results:
Residue pairs sorted by strength in covariance:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] gremlin predictions, sequence seperation > 3. The i and j are positions as given in the consensus sequence. Show Scaled Distribution

ij Raw Score Scaled Score
45_G99_G0.33262.750
86_S128_H0.30422.515
52_G99_G0.256762.123
55_G92_F0.242212.003
15_W18_N0.22371.850
7_N10_M0.22241.839
91_F95_L0.212991.761
98_I113_A0.20731.714
94_I145_L0.201851.669
100_F109_S0.201551.666
128_H138_Y0.195891.620
120_L127_T0.192961.595
104_T109_S0.191831.586
10_M119_N0.190921.579
139_P142_R0.189291.565
78_K83_E0.189171.564
125_V150_F0.18871.560
94_I117_V0.188091.555
55_G80_G0.180761.495
145_L150_F0.179411.483
128_H139_P0.178631.477
56_N97_W0.178171.473
61_L65_N0.177481.467
84_Y148_F0.174371.442
52_G95_L0.169071.398
58_Y62_I0.169031.398
45_G52_G0.166241.374
48_L99_G0.16381.354
101_A113_A0.159181.316
116_V120_L0.158981.314
88_P130_W0.158881.314
41_R105_G0.158061.307
62_I79_G0.158051.307
142_R147_P0.157231.300
56_N96_I0.154961.281
143_K148_F0.154281.276
117_V121_S0.150661.246
7_N11_N0.150011.240
116_V119_N0.151.240
125_V144_A0.147461.219
41_R102_L0.146231.209
50_L54_I0.145131.200
53_S56_N0.14471.196
97_W112_F0.143551.187
58_Y80_G0.143231.184
62_I65_N0.142421.178
87_C90_Y0.141391.169
74_Y134_K0.140621.163
121_S145_L0.139161.151
119_N122_P0.138991.149
4_P89_H0.138221.143
45_G48_L0.137111.134
76_I138_Y0.137071.133
85_V88_P0.136611.129
74_Y130_W0.136371.128
86_S144_A0.135291.119
6_S10_M0.132821.098
53_S96_I0.132261.094
54_I58_Y0.131521.087
2_T11_N0.131371.086
87_C127_T0.13021.076
131_Y135_F0.129911.074
128_H132_K0.129261.069
55_G59_S0.128571.063
10_M13_F0.128531.063
11_N14_F0.128221.060
135_F139_P0.12741.053
3_M8_V0.124451.029
79_G82_F0.12441.029
43_I47_A0.124331.028
120_L123_R0.12351.021
66_L77_P0.123081.018
143_K146_I0.122791.015
3_M11_N0.122221.011
86_S139_P0.122081.009
44_I47_A0.121551.005
146_I149_I0.12121.002
98_I110_Y0.120770.999
59_S92_F0.120370.995
3_M10_M0.119870.991
44_I136_K0.11920.986
11_N15_W0.11880.982
86_S138_Y0.118710.981
57_F60_H0.118420.979
116_V122_P0.118330.978
144_A150_F0.117980.975
43_I46_L0.117820.974
77_P93_E0.117520.972
64_A68_K0.117110.968
137_E142_R0.117050.968
24_Y27_S0.116960.967
79_G86_S0.11630.962
136_K149_I0.116010.959
85_V91_F0.115630.956
8_V12_C0.115440.954
40_P43_I0.114740.949
79_G83_E0.113830.941
143_K149_I0.113680.940
138_Y142_R0.113490.938
47_A54_I0.11280.933
76_I135_F0.112630.931
21_L25_Y0.112570.931
45_G102_L0.11240.929
52_G92_F0.110410.913
56_N63_L0.110320.912
137_E148_F0.110140.911
40_P50_L0.110030.910
40_P44_I0.109360.904
81_L92_F0.108630.898
68_K71_S0.108090.894
115_F119_N0.107880.892
59_S80_G0.107060.885
106_S109_S0.106470.880
13_F16_V0.10620.878
4_P112_F0.105720.874
44_I54_I0.105620.873
60_H96_I0.105540.873
112_F120_L0.105110.869
34_Y136_K0.105090.869
55_G81_L0.105050.869
8_V104_T0.103940.859
36_W42_F0.103920.859
56_N120_L0.103310.854
109_S112_F0.103170.853
65_N69_P0.103080.852
144_A147_P0.102750.850
136_K140_K0.102580.848
60_H89_H0.102550.848
75_K138_Y0.10240.847
5_I54_I0.102370.846
63_L77_P0.101890.842
84_Y143_K0.101870.842
101_A110_Y0.101570.840
6_S13_F0.101330.838
96_I99_G0.101190.837
24_Y28_Y0.101170.836
60_H63_L0.100820.834
56_N112_F0.100710.833
14_F60_H0.100670.832
57_F61_L0.100380.830
56_N89_H0.100130.828
48_L102_L0.09990.826
92_F123_R0.098990.818
88_P92_F0.098840.817
93_E97_W0.097920.810
60_H123_R0.097840.809
130_W134_K0.097540.806
77_P131_Y0.097470.806
56_N60_H0.097250.804
49_F146_I0.097150.803
120_L126_Q0.09710.803
70_G97_W0.09710.803
30_A133_K0.097010.802
3_M87_C0.09610.795
56_N116_V0.095950.793
97_W108_P0.095870.793
115_F120_L0.09580.792
114_L118_V0.095560.790
140_K143_K0.095120.786
113_A117_V0.095010.786
94_I113_A0.094860.784
71_S118_V0.094360.780
104_T124_A0.094270.779
22_I92_F0.09380.776
53_S57_F0.093770.775
2_T6_S0.093670.774
89_H127_T0.093580.774
56_N77_P0.093490.773
117_V132_K0.093370.772
3_M15_W0.093310.771
106_S140_K0.093220.771
120_L150_F0.093120.770
115_F127_T0.092380.764
18_N116_V0.091830.759
64_A99_G0.091330.755
2_T7_N0.091250.754
73_K134_K0.091180.754
96_I116_V0.090910.752
42_F78_K0.090790.751
132_K137_E0.090710.750
131_Y134_K0.090590.749
30_A36_W0.090530.749
84_Y88_P0.090470.748
10_M123_R0.090290.747
45_G103_L0.090190.746
120_L145_L0.090160.745
56_N93_E0.089980.744
51_I81_L0.089960.744
97_W100_F0.089940.744
55_G58_Y0.089650.741
87_C120_L0.08960.741
34_Y149_I0.089340.739
48_L54_I0.089330.739
87_C124_A0.089180.737
96_I100_F0.089130.737
123_R127_T0.088960.736
51_I140_K0.08890.735
55_G120_L0.088660.733
50_L136_K0.088570.732
62_I88_P0.088530.732
5_I34_Y0.088180.729
119_N123_R0.087950.727
34_Y47_A0.087560.724
77_P97_W0.087410.723
7_N119_N0.087050.720
116_V123_R0.086950.719
119_N127_T0.086940.719
48_L95_L0.086860.718
111_A115_F0.086350.714
77_P112_F0.086150.712
58_Y65_N0.086070.712
26_L100_F0.085880.710
7_N12_C0.085790.709
8_V11_N0.085710.709
119_N126_Q0.085520.707
30_A38_P0.085230.705
59_S62_I0.084820.701
143_K150_F0.084770.701
5_I17_L0.084660.700
21_L111_A0.084660.700
55_G108_P0.08460.699
Legend: The value of the raw score is the function of the learning procedure, L2 normalization and APC (entropic) correction. These are to be used for relative ranking only.
HHsearch Results:
Top (length/2) GREMLIN results overlayed on top 10 PDB hits:

Legend: The grey circles underneath are pdb residue contacts (min distance < 5 Angstroms). The coloring of these circles is based on HHsearch results which uses the overall probability, per-site alignment prob and agreement of top hits weighted by HHsearch score (Note we only consider monomeric contacts, there might be homo-oligomeric contacts in the pdb that are not shown.)
PDB Cov Prob HH_delta
4a2nB0.993399.40.647
4huqT0.60676.90.949
4djkA0.946.50.949
2ketA0.16674.50.953
2a9hA0.52.60.958
3l4yA0.80672.10.96
3lppA0.90671.70.961
3l1lA0.83331.60.962
1rpqW0.17331.60.962
3j00Z0.31331.20.965
If you are interested in a protein containing this domain,
drop us a note and we'll try to predict the structure for you.

Page generated in 0.0313 seconds.