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hENT1

ID: 1499269098 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 456 (430)
Sequences: 1427 (959.1)
Seq/Len: 3.319
Nf(neff/√len): 46.3

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 3.319).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
371_F402_A3.7361.00
297_C444_L3.3221.00
340_F401_A3.2561.00
24_G219_T2.4371.00
298_F369_L2.4321.00
400_M403_F2.1201.00
19_F178_S2.0801.00
341_D345_R2.0161.00
301_T448_F1.8750.99
87_C91_P1.8150.99
183_A428_E1.7730.99
302_I369_L1.7490.99
368_R406_S1.7350.99
418_G434_M1.7180.99
15_W175_P1.7070.99
141_V145_I1.6990.99
374_L399_F1.6790.99
283_K287_K1.6710.98
390_F399_F1.6670.98
371_F403_F1.6560.98
87_C148_V1.6160.98
300_F411_A1.6000.98
26_L30_N1.5910.98
30_N338_N1.5780.98
140_F144_M1.5680.97
121_L366_L1.5650.97
26_L368_R1.5530.97
288_N426_E1.5530.97
33_M85_T1.5270.97
26_L29_W1.5210.97
340_F404_A1.5170.97
173_T415_M1.5120.97
180_Q300_F1.5070.97
160_S285_I1.4960.96
297_C440_L1.4930.96
290_S294_F1.4920.96
375_L399_F1.4820.96
444_L448_F1.4780.96
160_S435_A1.4720.96
26_L304_I1.4710.96
137_L141_V1.4650.96
96_T100_S1.4630.96
181_G342_W1.4540.96
173_T177_M1.4530.96
413_L417_F1.4260.95
303_T368_R1.4250.95
174_A416_C1.4100.94
20_M222_C1.4020.94
114_G117_V1.4010.94
344_G368_R1.3840.94
185_F339_I1.3810.94
412_S416_C1.3770.94
314_V396_F1.3770.94
263_K266_S1.3690.93
341_D407_N1.3570.93
159_G163_G1.3540.93
297_C301_T1.3480.93
441_G445_G1.3350.92
162_F180_Q1.3280.92
20_M218_L1.3230.92
314_V375_L1.3080.91
293_A437_F1.3080.91
30_N303_T1.3030.91
293_A434_M1.3010.91
117_V120_L1.3000.91
411_A414_C1.3000.91
192_I330_P1.2940.90
307_F344_G1.2940.90
22_G30_N1.2910.90
160_S416_C1.2890.90
86_L90_L1.2880.90
295_S365_V1.2870.90
14_V27_L1.2740.89
303_T337_F1.2740.89
30_N33_M1.2670.89
19_F182_L1.2650.89
9_D12_K1.2610.89
310_V376_L1.2610.89
106_I110_V1.2570.89
438_L442_L1.2440.88
173_T176_I1.2380.88
125_I218_L1.2290.87
303_T407_N1.2210.87
37_Q327_Y1.2200.87
407_N411_A1.2190.87
415_M434_M1.2180.86
37_Q195_I1.2180.86
357_D437_F1.2180.86
406_S410_L1.2160.86
375_L403_F1.2100.86
303_T308_P1.2050.86
283_K286_L1.2010.85
304_I307_F1.2000.85
162_F173_T1.1980.85
369_L376_L1.1980.85
92_L439_C1.1870.84
411_A415_M1.1860.84
32_F143_T1.1830.84
124_L128_I1.1800.84
443_A447_V1.1770.84
144_M148_V1.1740.84
394_A398_F1.1720.83
107_P144_M1.1680.83
168_L185_F1.1680.83
341_D344_G1.1660.83
178_S342_W1.1640.83
304_I337_F1.1630.83
335_L339_I1.1630.83
189_V193_C1.1590.83
308_P445_G1.1580.82
217_I221_I1.1530.82
39_F43_L1.1520.82
211_T342_W1.1460.82
341_D368_R1.1450.81
415_M438_L1.1450.81
153_F404_A1.1400.81
91_P151_N1.1350.81
289_I434_M1.1320.80
364_L409_Y1.1300.80
214_A218_L1.1280.80
252_L255_K1.1260.80
37_Q191_M1.1220.80
281_S426_E1.1200.79
12_K16_L1.1190.79
181_G345_R1.1190.79
177_M183_A1.1180.79
18_F168_L1.1140.79
168_L172_Y1.1130.79
316_S393_D1.1130.79
34_T80_F1.1120.79
339_I343_L1.1120.79
33_M442_L1.1110.79
303_T307_F1.1080.78
191_M195_I1.1070.78
294_F444_L1.1060.78
302_I371_F1.1000.78
370_V374_L1.0990.78
262_G268_V1.0980.78
154_G296_V1.0940.77
38_Y368_R1.0930.77
214_A221_I1.0930.77
83_V144_M1.0920.77
177_M434_M1.0890.77
305_G452_F1.0890.77
15_W174_A1.0880.77
15_W416_C1.0880.77
177_M415_M1.0870.77
307_F334_F1.0860.76
111_R163_G1.0850.76
158_Q338_N1.0840.76
221_I398_F1.0830.76
39_F209_F1.0820.76
158_Q412_S1.0810.76
193_C207_G1.0760.76
228_R390_F1.0750.75
7_P234_Y1.0740.75
200_E380_I1.0720.75
245_E248_T1.0720.75
380_I456_V1.0720.75
39_F143_T1.0680.75
154_G412_S1.0680.75
40_T312_V1.0660.75
30_N184_G1.0600.74
404_A408_G1.0570.74
162_F411_A1.0560.74
304_I338_N1.0560.74
177_M431_G1.0550.74
7_P11_Y1.0530.73
7_P10_R1.0520.73
294_F298_F1.0520.73
308_P368_R1.0510.73
4_S398_F1.0500.73
145_I148_V1.0500.73
112_I161_L1.0490.73
26_L300_F1.0480.73
306_M375_L1.0440.72
18_F164_L1.0420.72
17_I21_L1.0370.72
260_R263_K1.0360.72
26_L371_F1.0330.71
37_Q44_D1.0300.71
181_G435_A1.0300.71
451_L454_A1.0280.71
5_H8_Q1.0270.71
113_L229_L1.0270.71
309_A335_L1.0270.71
296_V411_A1.0270.71
88_A151_N1.0260.71
343_L405_F1.0240.70
28_P38_Y1.0230.70
183_A328_F1.0230.70
26_L341_D1.0220.70
337_F403_F1.0210.70
36_T81_N1.0210.70
244_G247_E1.0160.69
26_L438_L1.0120.69
89_M442_L1.0110.69
252_L256_G1.0110.69
26_L415_M1.0110.69
193_C197_S1.0110.69
86_L97_Y1.0100.69
97_Y357_D1.0100.69
88_A368_R1.0080.69
304_I341_D1.0060.68
89_M301_T1.0050.68
164_L223_Y1.0050.68
183_A190_A1.0050.68
265_E269_S1.0030.68
376_L434_M1.0020.68
26_L307_F1.0020.68
195_I327_Y1.0010.68
281_S285_I1.0000.68
29_W89_M1.0000.68
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
4j05A20.767599.20.861Contact Map
4pypA10.899199.10.867Contact Map
1pw4A10.804899.10.87Contact Map
2cfqA10.802698.90.878Contact Map
3o7qA10.774198.80.883Contact Map
4xnjA1098.80.884Contact Map
4zw9A1098.70.884Contact Map
3wdoA10.771998.70.885Contact Map
4tphA20.815898.60.89Contact Map
2gfpA20.752298.50.892Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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