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ID: 1499455966 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 940 (877)
Sequences: 12733 (10823.5)
Seq/Len: 14.519
Nf(neff/√len): 365.5

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 14.519).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
638_L655_M4.7311.00
835_I874_G3.9481.00
540_V565_L3.8571.00
538_Q637_D3.4631.00
538_Q844_R3.2801.00
567_I576_Q3.2801.00
308_D336_R3.2601.00
657_P844_R3.2061.00
582_D643_Y2.9931.00
102_L118_S2.8421.00
875_R883_Q2.7691.00
520_D559_K2.7071.00
679_R704_T2.6191.00
524_N559_K2.6121.00
493_S520_D2.5311.00
542_A585_A2.5121.00
345_L353_D2.5061.00
818_I874_G2.4991.00
788_I816_V2.4991.00
832_I885_L2.4861.00
280_S290_V2.4711.00
71_T169_S2.4541.00
529_Y533_L2.4471.00
838_S867_I2.3981.00
495_Q499_S2.3941.00
298_L448_L2.3921.00
517_G521_R2.3511.00
526_I552_E2.3501.00
657_P660_G2.3401.00
535_V562_C2.3391.00
347_F452_V2.3351.00
820_D836_C2.3111.00
520_D628_S2.2941.00
660_G784_T2.2871.00
849_R852_L2.2641.00
816_V837_L2.2421.00
773_R793_P2.2271.00
237_Q357_N2.2231.00
495_Q498_L2.2171.00
426_M438_M2.2121.00
537_E637_D2.2081.00
524_N528_H2.2061.00
526_I556_G2.2001.00
435_A439_S2.2001.00
721_D725_K2.1991.00
539_S586_S2.1751.00
90_R94_R2.1741.00
535_V539_S2.1681.00
230_H364_G2.1421.00
818_I882_V2.1361.00
283_Y348_S2.1221.00
486_G494_E2.1101.00
92_L196_F2.1101.00
251_Y434_S2.1071.00
539_S562_C2.0811.00
540_V641_V2.0741.00
815_G860_H2.0451.00
894_V922_F2.0391.00
550_V686_F2.0321.00
296_G337_I2.0221.00
153_P180_A2.0121.00
95_R192_E2.0081.00
638_L844_R1.9881.00
678_S729_N1.9871.00
499_S502_S1.9861.00
663_N813_N1.9781.00
303_S444_I1.9701.00
641_V847_I1.9691.00
736_S758_E1.9391.00
176_G181_L1.9381.00
578_Y650_K1.9361.00
709_E712_E1.9321.00
503_L513_S1.9191.00
310_N334_P1.9141.00
426_M442_K1.9031.00
732_A786_F1.9001.00
892_V901_L1.8941.00
815_G838_S1.8831.00
92_L193_F1.8551.00
877_L883_Q1.8451.00
523_S559_K1.8361.00
860_H865_Y1.8171.00
832_I873_R1.8021.00
276_L287_W1.7881.00
543_I553_W1.7671.00
520_D524_N1.7631.00
580_L585_A1.7601.00
348_S457_D1.7561.00
706_C728_I1.7531.00
536_A539_S1.7521.00
679_R728_I1.7381.00
678_S703_A1.7251.00
231_E367_Y1.7241.00
514_I552_E1.7111.00
415_F424_L1.7111.00
834_E869_R1.7091.00
522_R549_E1.7071.00
86_F90_R1.7061.00
877_L881_N1.7021.00
506_Y704_T1.7011.00
540_V563_V1.7001.00
678_S751_K1.7001.00
102_L151_E1.6991.00
636_T659_Q1.6961.00
487_R490_R1.6921.00
501_Q517_G1.6911.00
451_T464_P1.6881.00
670_N812_E1.6821.00
660_G842_M1.6801.00
459_D462_Q1.6721.00
556_G560_A1.6671.00
777_N786_F1.6661.00
636_T657_P1.6601.00
690_V732_A1.6561.00
308_D338_P1.6521.00
507_S548_M1.6471.00
693_L786_F1.6461.00
295_I387_V1.6411.00
413_F426_M1.6391.00
191_R324_D1.6311.00
189_L326_M1.6251.00
162_P168_R1.6221.00
712_E718_Y1.6211.00
816_V835_I1.6191.00
715_I718_Y1.6101.00
455_Y458_F1.6091.00
684_S690_V1.6071.00
578_Y643_Y1.6051.00
528_H629_P1.6021.00
634_L637_D1.6021.00
488_V661_L1.5921.00
91_S94_R1.5901.00
858_V867_I1.5851.00
89_I156_F1.5811.00
571_Y579_M1.5771.00
96_H192_E1.5741.00
821_P825_A1.5721.00
73_D169_S1.5691.00
312_Y385_E1.5691.00
694_D732_A1.5691.00
708_A722_V1.5671.00
271_I424_L1.5661.00
543_I554_I1.5651.00
706_C722_V1.5611.00
494_E498_L1.5551.00
493_S559_K1.5541.00
294_L449_F1.5501.00
372_L376_A1.5481.00
520_D523_S1.5471.00
710_Y713_I1.5371.00
693_L732_A1.5351.00
664_F786_F1.5311.00
816_V874_G1.5301.00
506_Y726_N1.5251.00
56_K105_D1.5211.00
346_L349_D1.5191.00
732_A735_P1.5151.00
824_L836_C1.5131.00
591_D594_D1.5111.00
444_I470_Q1.5061.00
683_F734_T1.5041.00
722_V726_N1.5041.00
493_S523_S1.4951.00
85_I193_F1.4931.00
553_W589_V1.4921.00
369_Y373_A1.4891.00
535_V541_V1.4881.00
44_M113_F1.4871.00
732_A777_N1.4861.00
295_I413_F1.4831.00
503_L704_T1.4811.00
91_S200_D1.4731.00
553_W557_V1.4691.00
679_R706_C1.4611.00
544_M553_W1.4611.00
147_K150_K1.4591.00
289_R355_S1.4571.00
577_L581_Q1.4561.00
573_N577_L1.4531.00
514_I548_M1.4521.00
133_L136_Q1.4501.00
278_N282_K1.4491.00
909_A917_L1.4481.00
551_I695_W1.4481.00
296_G311_L1.4421.00
267_L430_P1.4381.00
334_P372_L1.4351.00
792_E806_L1.4351.00
664_F693_L1.4331.00
491_W662_S1.4281.00
852_L856_K1.4271.00
754_G777_N1.4221.00
300_A339_I1.4161.00
232_A236_S1.4111.00
905_E920_V1.4081.00
669_G701_S1.4060.99
356_K360_V1.4050.99
233_F361_A1.4040.99
489_H658_H1.3990.99
633_T637_D1.3990.99
230_H366_V1.3980.99
565_L583_S1.3970.99
231_E375_R1.3970.99
582_D849_R1.3920.99
654_V843_A1.3920.99
908_I931_A1.3870.99
492_I496_A1.3860.99
549_E591_D1.3820.99
293_A350_L1.3790.99
756_G786_F1.3740.99
231_E235_I1.3720.99
571_Y687_S1.3700.99
277_K417_E1.3690.99
582_D650_K1.3690.99
681_L708_A1.3670.99
269_M439_S1.3660.99
787_T837_L1.3640.99
371_D374_R1.3640.99
446_I450_D1.3630.99
294_L452_V1.3610.99
850_T854_E1.3510.99
338_P345_L1.3500.99
241_T310_N1.3480.99
103_F180_A1.3480.99
85_I172_I1.3480.99
563_V638_L1.3310.99
337_I354_V1.3310.99
732_A756_G1.3310.99
665_V700_T1.3240.99
503_L515_T1.3240.99
439_S443_E1.3230.99
464_P471_S1.3180.99
84_R200_D1.3170.99
228_A232_A1.3140.99
235_I375_R1.3130.99
811_L814_T1.3110.99
503_L506_Y1.3090.99
860_H867_I1.3010.99
581_Q650_K1.2990.99
297_V350_L1.2990.99
492_I555_L1.2980.99
690_V786_F1.2970.99
250_P437_R1.2970.99
590_L594_D1.2920.99
792_E881_N1.2870.99
486_G490_R1.2870.99
873_R888_M1.2850.99
915_V918_V1.2790.99
339_I345_L1.2790.99
689_D780_P1.2750.99
836_C869_R1.2750.99
141_L175_V1.2720.99
761_S764_L1.2710.99
783_F837_L1.2710.99
367_Y375_R1.2680.99
287_W422_V1.2670.99
293_A354_V1.2650.99
137_D159_S1.2620.99
211_Y214_F1.2620.99
690_V756_G1.2610.99
656_L843_A1.2610.99
505_L548_M1.2550.99
681_L723_I1.2530.99
817_D860_H1.2500.99
682_Q707_F1.2500.99
279_L453_A1.2500.99
664_F785_V1.2480.99
78_D158_I1.2450.98
818_I835_I1.2430.98
843_A857_F1.2410.98
538_Q638_L1.2400.98
76_Q170_T1.2400.98
508_A709_E1.2380.98
690_V693_L1.2370.98
809_E881_N1.2360.98
684_S732_A1.2350.98
448_L463_F1.2350.98
214_F217_F1.2300.98
97_G210_N1.2300.98
564_F692_I1.2290.98
267_L435_A1.2280.98
340_A345_L1.2270.98
874_G884_I1.2260.98
583_S643_Y1.2250.98
489_H633_T1.2250.98
491_W666_C1.2210.98
675_D678_S1.2200.98
303_S441_L1.2180.98
752_S775_L1.2180.98
664_F694_D1.2180.98
698_A705_L1.2170.98
440_E444_I1.2150.98
196_F200_D1.2120.98
91_S196_F1.2080.98
829_T876_I1.2080.98
693_L777_N1.2050.98
760_C776_H1.2030.98
664_F784_T1.1960.98
141_L144_V1.1960.98
505_L551_I1.1950.98
924_L927_N1.1900.98
542_A580_L1.1880.98
515_T518_E1.1860.98
298_L445_F1.1850.98
109_T112_C1.1850.98
664_F756_G1.1820.98
428_Y438_M1.1810.98
894_V899_V1.1790.98
136_Q140_K1.1780.98
492_I520_D1.1780.98
756_G784_T1.1760.98
909_A918_V1.1740.98
77_R84_R1.1710.98
664_F732_A1.1680.97
51_L328_H1.1670.97
297_V448_L1.1660.97
902_G905_E1.1640.97
828_P831_T1.1630.97
661_L785_V1.1620.97
783_F787_T1.1560.97
340_A344_A1.1560.97
698_A703_A1.1550.97
434_S437_R1.1550.97
283_Y352_R1.1530.97
233_F237_Q1.1530.97
141_L157_L1.1500.97
458_F462_Q1.1450.97
578_Y582_D1.1440.97
237_Q360_V1.1420.97
148_L179_I1.1410.97
101_S117_F1.1400.97
692_I785_V1.1390.97
507_S510_L1.1330.97
369_Y372_L1.1330.97
674_L703_A1.1320.97
292_T311_L1.1310.97
523_S556_G1.1290.97
732_A737_A1.1280.97
730_F752_S1.1270.97
683_F733_I1.1270.97
817_D862_E1.1270.97
783_F842_M1.1260.97
656_L784_T1.1220.97
739_A764_L1.1210.97
655_M847_I1.1200.96
101_S115_K1.1200.96
285_T289_R1.1190.96
275_T454_K1.1190.96
383_L386_Q1.1180.96
817_D838_S1.1170.96
694_D786_F1.1160.96
236_S239_Q1.1160.96
912_C915_V1.1150.96
890_R905_E1.1140.96
452_V463_F1.1130.96
678_S730_F1.1130.96
694_D777_N1.1130.96
433_F438_M1.1120.96
158_I172_I1.1090.96
156_F172_I1.1090.96
535_V560_A1.1090.96
533_L587_T1.1090.96
493_S524_N1.1080.96
47_P50_A1.1040.96
103_F179_I1.1040.96
506_Y513_S1.1030.96
668_Y693_L1.1010.96
496_A520_D1.0990.96
261_N431_K1.0950.96
762_E766_E1.0930.96
477_P480_V1.0880.96
755_L760_C1.0880.96
275_T450_D1.0880.96
573_N597_A1.0860.96
566_V781_T1.0850.96
462_Q825_A1.0850.96
752_S773_R1.0840.96
291_V422_V1.0830.96
674_L678_S1.0830.96
920_V931_A1.0830.96
683_F786_F1.0820.96
659_Q840_L1.0810.95
545_K591_D1.0780.95
558_L639_A1.0760.95
837_L870_T1.0740.95
683_F737_A1.0740.95
108_K112_C1.0740.95
413_F424_L1.0720.95
49_E219_R1.0700.95
358_L362_K1.0680.95
344_A461_F1.0650.95
372_L375_R1.0640.95
574_E650_K1.0630.95
727_Q749_S1.0610.95
682_Q694_D1.0600.95
738_L755_L1.0600.95
191_R195_A1.0590.95
233_F360_V1.0590.95
271_I442_K1.0590.95
681_L722_V1.0580.95
733_I738_L1.0550.95
76_Q85_I1.0530.95
87_W200_D1.0520.95
279_L283_Y1.0520.95
554_I695_W1.0510.94
71_T171_A1.0510.94
836_C858_V1.0500.94
669_G697_I1.0500.94
542_A567_I1.0480.94
624_N627_E1.0480.94
664_F668_Y1.0470.94
499_S517_G1.0460.94
130_P163_D1.0460.94
891_Q900_E1.0460.94
691_S695_W1.0450.94
664_F787_T1.0450.94
276_L290_V1.0430.94
821_P827_V1.0430.94
876_I882_V1.0430.94
529_Y532_S1.0390.94
574_E578_Y1.0380.94
522_R552_E1.0360.94
489_H558_L1.0320.94
811_L841_N1.0300.94
98_I101_S1.0280.94
443_E467_Q1.0270.94
156_F174_I1.0260.94
292_T296_G1.0240.93
295_I389_I1.0230.93
815_G865_Y1.0220.93
289_R359_S1.0210.93
522_R525_Q1.0200.93
75_S168_R1.0200.93
386_Q389_I1.0190.93
907_V911_T1.0180.93
387_V390_T1.0170.93
390_T393_P1.0150.93
640_Y656_L1.0140.93
55_Y60_E1.0130.93
548_M552_E1.0110.93
349_D352_R1.0100.93
490_R493_S1.0100.93
285_T351_V1.0090.93
683_F713_I1.0080.93
703_A729_N1.0070.93
45_S211_Y1.0060.93
665_V697_I1.0040.93
269_M442_K1.0030.93
371_D375_R1.0010.92
735_P738_L1.0010.92
355_S359_S1.0010.92
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
2vsqA10.92341000.223Contact Map
4dg8A10.47231000.574Contact Map
1amuA20.48191000.59Contact Map
4iz6A20.48721000.598Contact Map
3tsyA10.43511000.599Contact Map
4d4iA101000.599Contact Map
3wvnA20.41000.608Contact Map
4gr5A40.48191000.61Contact Map
3g7sA20.47131000.61Contact Map
4r0mB20.47981000.613Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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