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ETR1

ID: 1499823886 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 96 (96)
Sequences: 306 (213)
Seq/Len: 3.188
Nf(neff/√len): 21.7

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 3.188).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
17_F51_A3.0171.00
55_I74_A2.1591.00
13_A54_L2.1431.00
20_P46_I1.9841.00
56_N75_K1.6890.98
10_F57_L1.6810.98
42_F46_I1.6590.98
35_Y70_V1.5910.98
9_D75_K1.5790.97
34_P43_G1.4700.96
33_F93_I1.4600.95
61_T66_T1.4600.95
72_T76_V1.4150.94
61_T68_A1.3960.94
84_A95_D1.3000.90
38_V48_L1.2950.90
21_L81_V1.2560.88
34_P90_V1.2510.88
46_I49_C1.2420.87
12_I79_A1.2410.87
23_L39_L1.2410.87
91_H95_D1.2380.87
41_Q81_V1.2150.85
46_I52_T1.1960.84
46_I78_T1.1920.84
45_F53_H1.1920.84
35_Y95_D1.1900.84
22_E90_V1.1780.83
36_R48_L1.1740.83
19_I85_T1.1650.82
27_V36_R1.1640.82
34_P47_V1.1610.82
11_F83_C1.1440.80
6_Y60_F1.1210.79
58_W63_H1.1010.77
56_N71_M1.0980.76
53_H82_S1.0930.76
16_Y53_H1.0930.76
31_A93_I1.0840.75
44_A81_V1.0610.73
10_F55_I1.0530.72
78_T82_S1.0530.72
41_Q61_T1.0520.72
38_V81_V1.0510.72
13_A86_A1.0470.72
16_Y85_T1.0370.71
84_A88_M1.0300.70
53_H85_T1.0050.67
20_P49_C1.0010.67
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
2kzqA10.27080.80.939Contact Map
2kdrX10.27080.70.94Contact Map
2zhyA30.29170.70.941Contact Map
1pv5A10.47920.70.941Contact Map
2nt8A10.32290.70.942Contact Map
3ci3A10.38540.60.943Contact Map
1wvtA30.30210.60.943Contact Map
4fasA300.60.945Contact Map
2mc7A10.31250.60.946Contact Map
3fzeA10.27080.50.946Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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