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Beta cardiac myosin_1

ID: 1500510029 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 960 (918)
Sequences: 4465 (2513)
Seq/Len: 4.864
Nf(neff/√len): 82.9

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 4.864).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
124_T187_N3.1121.00
553_F563_F3.0781.00
549_K590_I3.0431.00
794_G798_R3.0291.00
715_Y758_F3.0231.00
518_Q522_D3.0171.00
112_W129_K2.8891.00
345_E442_R2.8851.00
534_L548_F2.7521.00
173_S665_T2.7371.00
90_M104_N2.6871.00
484_K525_E2.5961.00
285_I332_T2.5391.00
537_E551_K2.5261.00
294_K297_L2.5041.00
354_G358_H2.4351.00
545_D592_G2.4291.00
320_V323_I2.4191.00
774_E777_R2.4171.00
186_V189_K2.3581.00
820_A824_V2.3541.00
498_Q508_W2.3351.00
815_Q819_R2.2751.00
232_N240_N2.2531.00
357_M361_N2.1801.00
154_I187_N2.1401.00
789_Q793_R2.1361.00
117_Y156_S2.1321.00
700_E703_R2.1081.00
97_H100_A2.0811.00
677_E688_L2.0651.00
479_N483_E2.0311.00
461_D464_G1.9661.00
297_L333_D1.9551.00
234_K239_D1.9501.00
721_R774_E1.9351.00
333_D350_Y1.8881.00
337_D346_K1.8701.00
580_I585_I1.8511.00
311_A315_Q1.8361.00
578_S587_D1.8311.00
359_F362_M1.8201.00
353_T356_I1.8031.00
577_F581_H1.8021.00
416_N419_Q1.7871.00
231_G244_F1.7861.00
478_I482_N1.7741.00
312_F428_A1.7681.00
161_A174_I1.7681.00
180_S184_K1.7671.00
281_R325_D1.7661.00
433_E437_N1.7441.00
473_F477_C1.7441.00
537_E541_P1.7321.00
269_E272_R1.7311.00
355_A388_M1.7281.00
336_F349_M1.7251.00
346_K350_Y1.7111.00
342_T345_E1.6970.99
678_T683_V1.6640.99
288_Q332_T1.6620.99
240_N243_R1.6590.99
180_S185_T1.6550.99
228_E232_N1.6400.99
583_A586_V1.6390.99
280_E284_H1.6270.99
228_E240_N1.6230.99
505_G760_H1.6190.99
479_N482_N1.6180.99
289_I293_K1.6100.99
324_D327_E1.6090.99
500_E762_K1.6090.99
475_Q478_I1.6080.99
390_L395_L1.6050.99
235_T238_N1.6040.99
235_T243_R1.6020.99
341_F346_K1.6000.99
385_A395_L1.5910.99
292_N298_L1.5870.99
238_N243_R1.5830.99
475_Q483_E1.5760.99
232_N235_T1.5740.99
306_N309_D1.5690.99
696_N701_G1.5660.99
544_T594_L1.5620.99
312_F360_G1.5550.99
533_I536_E1.5530.99
276_Q282_D1.5440.99
478_I483_E1.5430.99
232_N244_F1.5410.99
581_H588_Y1.5390.99
737_D740_K1.5300.99
349_M435_M1.5280.99
333_D346_K1.5250.99
695_C705_C1.5160.99
798_R802_K1.5100.98
596_K599_D1.5080.98
588_Y597_N1.5070.98
537_E547_T1.4990.98
382_D392_S1.4990.98
178_G184_K1.4980.98
300_M347_N1.4980.98
159_D163_Q1.4950.98
471_N482_N1.4940.98
479_N486_Q1.4930.98
543_A547_T1.4880.98
474_E486_Q1.4870.98
159_D194_Y1.4870.98
478_I485_L1.4720.98
181_G185_T1.4720.98
461_D465_F1.4720.98
391_N394_D1.4700.98
461_D466_E1.4700.98
490_N494_F1.4680.98
765_F768_G1.4670.98
310_Y361_N1.4650.98
616_L620_L1.4590.98
605_V609_Y1.4570.98
247_F458_G1.4530.98
178_G183_G1.4530.98
547_T551_K1.4520.98
684_M689_V1.4450.98
700_E704_I1.4400.98
182_A185_T1.4390.98
180_S183_G1.4380.98
192_I250_I1.4380.98
501_Y504_E1.4380.98
593_W597_N1.4370.98
442_R445_A1.4350.98
673_I689_V1.4340.98
118_S121_F1.4340.98
499_E503_K1.4330.98
474_E478_I1.4330.98
181_G184_K1.4290.98
680_S683_V1.4260.98
530_I548_F1.4260.98
487_Q495_V1.4220.98
578_S585_I1.4170.97
274_I429_K1.4160.97
249_R264_E1.4100.97
530_I552_L1.4100.97
475_Q482_N1.4090.97
281_R284_H1.4080.97
672_C676_N1.4040.97
293_K298_L1.4040.97
808_R812_L1.4030.97
356_I428_A1.4000.97
238_N242_S1.3960.97
304_T309_D1.3940.97
380_E384_S1.3920.97
471_N475_Q1.3900.97
179_E184_K1.3890.97
489_F501_Y1.3860.97
768_G771_G1.3770.97
267_L270_K1.3760.97
231_G240_N1.3740.97
284_H287_Y1.3730.97
178_G181_G1.3730.97
232_N238_N1.3690.97
577_F583_A1.3690.97
478_I486_Q1.3610.97
86_K104_N1.3580.97
483_E486_Q1.3560.96
537_E543_A1.3490.96
479_N485_L1.3440.96
795_V799_M1.3420.96
179_E185_T1.3410.96
224_N228_E1.3400.96
280_E287_Y1.3400.96
474_E483_E1.3390.96
240_N244_F1.3360.96
389_G613_S1.3360.96
337_D343_S1.3340.96
462_I466_E1.3340.96
154_I670_V1.3330.96
217_E221_I1.3300.96
791_Q794_G1.3280.96
548_F552_L1.3280.96
541_P547_T1.3260.96
224_N237_R1.3250.96
740_K743_E1.3230.96
186_V190_R1.3170.96
302_L386_Y1.3130.96
488_F669_F1.3120.96
417_V421_I1.3080.95
689_V692_Q1.3080.95
343_S347_N1.3080.95
179_E182_A1.3060.95
484_K487_Q1.3010.95
577_F582_Y1.3000.95
226_A332_T1.2990.95
817_N820_A1.2990.95
462_I465_F1.2990.95
475_Q486_Q1.2980.95
240_N246_K1.2970.95
472_S596_K1.2970.95
250_I258_L1.2930.95
812_L816_W1.2920.95
432_Y436_F1.2850.95
547_T594_L1.2840.95
103_Y106_K1.2840.95
178_G185_T1.2830.95
480_F525_E1.2820.95
796_L800_E1.2760.95
235_T240_N1.2740.94
466_E475_Q1.2740.94
777_R781_L1.2720.94
693_L698_V1.2720.94
492_H496_L1.2710.94
471_N479_N1.2710.94
818_I822_M1.2700.94
919_V922_M1.2680.94
462_I468_F1.2670.94
517_L521_I1.2650.94
466_E471_N1.2640.94
355_A384_S1.2630.94
474_E479_N1.2600.94
420_V424_T1.2600.94
860_K864_E1.2550.94
425_G428_A1.2520.94
532_S652_R1.2520.94
249_R262_D1.2500.94
233_A238_N1.2450.94
233_A243_R1.2420.93
540_F547_T1.2420.93
381_A384_S1.2420.93
357_M360_G1.2410.93
497_E501_Y1.2390.93
498_Q501_Y1.2390.93
159_D165_M1.2350.93
106_K686_N1.2350.93
476_L479_N1.2350.93
436_F440_V1.2330.93
581_H584_G1.2310.93
500_E508_W1.2300.93
152_P155_F1.2300.93
475_Q479_N1.2290.93
811_L815_Q1.2280.93
485_L489_F1.2270.93
540_F543_A1.2250.93
501_Y508_W1.2240.93
228_E231_G1.2210.93
695_C701_G1.2200.93
229_A288_Q1.2200.93
486_Q489_F1.2190.93
460_L466_E1.2180.93
773_L777_R1.2160.93
746_L763_V1.2110.92
465_F468_F1.2100.92
115_Y124_T1.2100.92
116_T119_G1.2090.92
351_K614_L1.2070.92
703_R706_R1.2060.92
814_I818_I1.2060.92
676_N679_K1.1990.92
581_H586_V1.1980.92
482_N486_Q1.1970.92
153_H162_Y1.1960.92
328_E331_A1.1950.92
266_Y269_E1.1950.92
463_A466_E1.1920.92
709_F757_K1.1920.92
540_F594_L1.1900.91
238_N241_S1.1880.91
348_S614_L1.1860.91
152_P158_S1.1860.91
516_D521_I1.1850.91
576_H589_N1.1840.91
310_Y358_H1.1800.91
530_I579_L1.1790.91
105_L125_V1.1790.91
258_L261_A1.1780.91
673_I676_N1.1780.91
537_E544_T1.1760.91
233_A240_N1.1760.91
426_A429_K1.1750.91
476_L483_E1.1740.91
400_C415_Q1.1730.91
152_P156_S1.1720.91
108_R115_Y1.1700.90
475_Q485_L1.1700.90
281_R287_Y1.1690.90
312_F425_G1.1680.90
232_N243_R1.1660.90
179_E186_V1.1650.90
280_E283_Y1.1650.90
355_A387_L1.1650.90
231_G235_T1.1630.90
669_F672_C1.1630.90
108_R116_T1.1610.90
540_F544_T1.1600.90
811_L814_I1.1590.90
233_A241_S1.1570.90
232_N241_S1.1560.90
142_Y153_H1.1550.90
543_A551_K1.1540.90
153_H161_A1.1540.90
341_F345_E1.1540.90
89_D93_L1.1520.90
714_L717_D1.1510.90
270_K599_D1.1500.89
708_G712_R1.1500.89
192_I196_A1.1490.89
745_L749_L1.1480.89
180_S189_K1.1470.89
231_G436_F1.1440.89
739_R743_E1.1430.89
145_K149_E1.1420.89
431_V617_L1.1410.89
812_L815_Q1.1400.89
310_Y313_I1.1400.89
477_C483_E1.1380.89
693_L696_N1.1360.89
548_F590_I1.1320.88
818_I821_F1.1310.88
108_R114_I1.1300.88
697_G704_I1.1290.88
711_N768_G1.1270.88
153_H158_S1.1250.88
701_G704_I1.1250.88
302_L383_K1.1230.88
231_G243_R1.1210.88
162_Y165_M1.1210.88
186_V227_L1.1210.88
478_I489_F1.1190.88
657_K660_T1.1180.88
165_M252_F1.1170.88
116_T127_P1.1170.88
458_G462_I1.1170.88
489_F497_E1.1170.88
240_N284_H1.1160.88
155_F193_Q1.1140.87
482_N485_L1.1130.87
793_R797_A1.1120.87
460_L465_F1.1120.87
672_C675_P1.1110.87
480_F485_L1.1110.87
105_L108_R1.1100.87
541_P544_T1.1090.87
165_M172_Q1.1080.87
336_F341_F1.1070.87
272_R280_E1.1070.87
460_L464_G1.1060.87
424_T428_A1.1050.87
698_V701_G1.1050.87
492_H667_P1.1040.87
765_F770_L1.1020.87
536_E540_F1.1010.87
595_Q599_D1.1000.86
486_Q490_N1.1000.86
172_Q176_I1.0990.86
377_G381_A1.0990.86
358_H380_E1.0990.86
241_S244_F1.0980.86
533_I537_E1.0980.86
696_N705_C1.0980.86
786_T790_A1.0950.86
759_G764_F1.0950.86
490_N497_E1.0950.86
228_E246_K1.0940.86
617_L620_L1.0940.86
472_S475_Q1.0920.86
490_N493_M1.0910.86
480_F483_E1.0900.86
397_K412_T1.0890.86
234_K237_R1.0890.86
463_A467_I1.0840.85
435_M438_W1.0830.85
669_F676_N1.0830.85
592_G595_Q1.0820.85
471_N474_E1.0820.85
280_E286_F1.0800.85
471_N478_I1.0790.85
235_T241_S1.0740.85
88_E113_M1.0740.85
159_D198_I1.0740.85
323_I328_E1.0730.85
283_Y287_Y1.0720.85
178_G182_A1.0710.84
420_V425_G1.0710.84
468_F471_N1.0700.84
404_V413_K1.0690.84
495_V518_Q1.0690.84
609_Y612_S1.0680.84
491_H495_V1.0670.84
488_F492_H1.0660.84
474_E485_L1.0650.84
289_I353_T1.0640.84
743_E747_S1.0640.84
237_R240_N1.0640.84
231_G242_S1.0640.84
564_Q585_I1.0640.84
813_V816_W1.0620.84
93_L96_L1.0610.84
176_I182_A1.0600.84
697_G700_E1.0600.84
790_A794_G1.0580.83
785_I788_I1.0580.83
816_W820_A1.0570.83
143_R159_D1.0560.83
671_R676_N1.0560.83
474_E489_F1.0530.83
86_K90_M1.0520.83
389_G614_L1.0510.83
224_N232_N1.0500.83
705_C710_P1.0500.83
230_F243_R1.0490.83
291_S325_D1.0490.83
647_V651_H1.0480.83
179_E183_G1.0480.83
233_A244_F1.0470.83
529_G534_L1.0440.82
293_K297_L1.0430.82
185_T189_K1.0420.82
695_C706_R1.0410.82
41_D47_V1.0380.82
655_L659_M1.0370.82
480_F487_Q1.0330.81
692_Q697_G1.0330.81
542_K594_L1.0320.81
106_K110_G1.0320.81
925_R929_E1.0310.81
693_L701_G1.0290.81
829_W832_L1.0290.81
98_E694_R1.0290.81
688_L706_R1.0280.81
792_S796_L1.0270.81
190_R193_Q1.0270.81
473_F648_S1.0270.81
248_I263_I1.0260.81
494_F497_E1.0260.81
655_L658_L1.0250.81
358_H383_K1.0240.81
432_Y444_N1.0210.80
788_I791_Q1.0200.80
268_L272_R1.0180.80
490_N513_F1.0160.80
814_I817_N1.0150.80
497_E508_W1.0140.80
52_V62_E1.0140.80
861_E865_K1.0140.80
564_Q580_I1.0130.80
519_A562_N1.0120.80
440_V444_N1.0120.80
188_T192_I1.0120.80
87_I93_L1.0110.79
157_I161_A1.0110.79
187_N192_I1.0110.79
500_E503_K1.0100.79
228_E244_F1.0090.79
842_S846_E1.0090.79
473_F483_E1.0080.79
221_I224_N1.0080.79
119_G127_P1.0070.79
179_E189_K1.0070.79
780_R783_R1.0060.79
517_L523_L1.0060.79
907_Q911_N1.0040.79
521_I524_I1.0040.79
544_T547_T1.0040.79
191_V250_I1.0040.79
266_Y270_K1.0030.79
786_T789_Q1.0020.79
799_M802_K1.0020.79
866_S870_R1.0020.79
91_A100_A1.0000.78
294_K333_D1.0000.78
514_G517_L1.0000.78
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
1i84S20.9313100-0.014Contact Map
1kk8A10.82291000.013Contact Map
2dfsA20.88021000.042Contact Map
4pa0A201000.058Contact Map
4qbdA20.81871000.063Contact Map
2ycuA10.93751000.08Contact Map
1g8xA20.97291000.081Contact Map
1w7jA10.77191000.092Contact Map
1w9iA10.7251000.101Contact Map
4anjA10.86981000.103Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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