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EXOC7

ID: 1501011585 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 735 (614)
Sequences: 675 (463.6)
Seq/Len: 1.099
Nf(neff/√len): 18.7

Jackhmmer Results: (2016_04)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.099).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
378_E382_L3.4311.00
382_L386_I3.2561.00
378_E381_L3.2421.00
378_E385_I3.1631.00
379_Y382_L3.1271.00
378_E386_I3.0711.00
379_Y386_I2.9301.00
378_E387_P2.7451.00
379_Y385_I2.5130.99
382_L385_I2.4310.99
381_L385_I2.3380.99
379_Y387_P2.2780.99
379_Y383_A2.2320.98
49_F52_R2.2020.98
497_G572_L2.1980.98
378_E383_A2.1480.98
383_A386_I1.9580.96
382_L387_P1.9520.96
656_F703_F1.8660.94
381_L387_P1.7820.92
381_L386_I1.7800.92
593_L681_R1.7720.91
503_T580_N1.7390.90
410_G464_L1.6790.88
561_L688_Q1.6790.88
46_L53_L1.6390.87
377_S385_I1.6360.86
202_I598_A1.6340.86
132_D135_E1.6310.86
472_G476_L1.6250.86
77_V109_L1.5910.84
653_K714_K1.5880.84
23_E32_L1.5810.84
28_I38_L1.5760.84
136_L584_I1.5560.82
708_F711_N1.5340.81
714_K717_K1.5310.81
564_S665_E1.5060.80
112_Y372_V1.4920.79
660_N715_Y1.4920.79
656_F716_I1.4540.76
513_L524_L1.4440.75
154_F199_V1.4410.75
217_N221_Q1.4390.75
579_N619_Y1.4340.75
656_F660_N1.4340.75
101_I442_T1.4270.74
81_L654_E1.4240.74
101_I104_G1.4220.74
653_K656_F1.4220.74
45_I53_L1.4210.74
150_L158_M1.4120.73
45_I49_F1.4120.73
32_L46_L1.4100.73
656_F711_N1.4060.73
234_L681_R1.3910.72
178_D675_I1.3880.71
45_I52_R1.3850.71
34_K664_E1.3830.71
49_F53_L1.3750.70
45_I654_E1.3700.70
675_I694_E1.3630.69
626_V662_G1.3620.69
442_T597_V1.3560.69
514_L649_R1.3490.68
50_E53_L1.3480.68
91_Y108_R1.3480.68
193_E435_I1.3380.67
158_M411_E1.3380.67
352_G688_Q1.3370.67
19_K119_I1.3280.66
357_L360_E1.3130.65
395_F413_I1.3120.65
649_R714_K1.3120.65
560_L580_N1.3120.65
576_F580_N1.3110.65
170_I173_L1.2940.63
379_Y384_D1.2920.63
52_R162_S1.2900.63
654_E699_F1.2900.63
507_I575_I1.2860.63
175_S179_D1.2840.63
433_F509_F1.2830.62
677_D680_Q1.2800.62
140_K564_S1.2760.62
497_G567_Y1.2750.62
629_Y658_G1.2750.62
535_S539_E1.2730.62
676_P681_R1.2720.61
497_G673_W1.2660.61
650_Q700_L1.2630.61
377_S381_L1.2610.60
510_L552_V1.2600.60
576_F579_N1.2540.60
591_S656_F1.2510.60
158_M676_P1.2500.59
559_N563_K1.2480.59
536_Y539_E1.2460.59
372_V506_A1.2420.59
696_Y731_F1.2410.59
62_P496_D1.2400.59
115_S209_Y1.2350.58
371_F461_L1.2340.58
67_T70_L1.2300.58
181_E184_E1.2300.58
694_E703_F1.2250.57
42_M50_E1.2250.57
49_F56_L1.2250.57
525_A716_I1.2240.57
146_G158_M1.2210.57
623_W723_V1.2180.57
164_V239_H1.2070.55
28_I109_L1.2050.55
553_L597_V1.2020.55
154_F596_L1.2000.55
62_P70_L1.1990.55
710_K715_Y1.1990.55
164_V727_I1.1970.55
134_P439_L1.1960.54
154_F556_L1.1950.54
474_K478_D1.1870.54
689_K693_K1.1860.53
42_M49_F1.1800.53
219_Y223_R1.1730.52
609_R675_I1.1730.52
497_G718_Y1.1720.52
357_L361_T1.1700.52
478_D499_V1.1690.52
458_K715_Y1.1660.52
60_I164_V1.1660.52
84_L92_H1.1640.51
486_D563_K1.1640.51
377_S380_Q1.1630.51
534_T537_S1.1630.51
660_N703_F1.1600.51
653_K727_I1.1590.51
146_G608_Y1.1480.50
694_E698_A1.1470.50
575_I676_P1.1460.50
112_Y231_I1.1450.50
581_Y608_Y1.1450.50
19_K22_E1.1430.49
78_E596_L1.1390.49
534_T538_S1.1390.49
111_E721_E1.1380.49
114_G658_G1.1360.49
504_S511_Q1.1350.49
428_T433_F1.1340.49
523_M596_L1.1290.48
62_P98_E1.1280.48
83_C727_I1.1270.48
154_F203_S1.1250.48
107_G715_Y1.1230.48
565_K573_S1.1220.47
479_F513_L1.1220.47
666_L672_A1.1220.47
234_L516_F1.1210.47
635_L651_I1.1210.47
564_S576_F1.1180.47
153_E569_D1.1170.47
585_L660_N1.1170.47
176_G720_V1.1160.47
498_T676_P1.1140.47
112_Y630_I1.1120.46
78_E108_R1.1090.46
675_I684_I1.1090.46
168_V719_G1.1030.46
606_R633_K1.1030.46
622_S668_K1.1020.46
476_L509_F1.1020.46
572_L576_F1.1020.46
700_L720_V1.1020.46
677_D684_I1.1010.45
406_L438_H1.1010.45
513_L593_L1.1010.45
563_K576_F1.1000.45
177_D180_L1.1000.45
439_L649_R1.1000.45
33_E38_L1.0970.45
88_I91_Y1.0970.45
53_L373_K1.0960.45
175_S178_D1.0950.45
150_L584_I1.0930.45
64_H73_L1.0900.44
412_N505_N1.0870.44
136_L187_T1.0870.44
434_P516_F1.0850.44
430_L471_I1.0840.44
502_L582_N1.0820.44
56_L467_S1.0820.44
361_T572_L1.0810.44
440_K664_E1.0800.43
621_R625_K1.0780.43
49_F489_K1.0770.43
719_G722_Q1.0770.43
406_L460_K1.0770.43
70_L378_E1.0730.43
157_L410_G1.0700.43
383_A387_P1.0680.42
593_L655_R1.0670.42
126_F623_W1.0670.42
424_H487_P1.0660.42
81_L91_Y1.0650.42
474_K477_E1.0650.42
116_M445_E1.0650.42
579_N583_Y1.0640.42
472_G502_L1.0620.42
549_I593_L1.0620.42
595_Q693_K1.0600.42
520_A523_M1.0590.41
502_L513_L1.0570.41
666_L692_V1.0570.41
199_V627_T1.0560.41
208_E682_D1.0540.41
23_E28_I1.0530.41
395_F684_I1.0510.41
383_A391_Q1.0500.41
354_D508_L1.0470.40
358_D476_L1.0460.40
586_K717_K1.0460.40
175_S720_V1.0420.40
617_Q714_K1.0420.40
202_I234_L1.0400.40
230_S374_L1.0400.40
411_E414_V1.0400.40
378_E384_D1.0400.40
497_G579_N1.0390.40
386_I395_F1.0360.39
665_E728_D1.0360.39
713_E727_I1.0360.39
500_H567_Y1.0350.39
225_S240_K1.0340.39
622_S670_Q1.0340.39
62_P76_N1.0330.39
618_T665_E1.0320.39
105_P109_L1.0300.39
433_F520_A1.0300.39
76_N105_P1.0290.39
351_A515_D1.0290.39
685_R732_D1.0290.39
698_A703_F1.0290.39
171_L174_I1.0260.38
164_V230_S1.0250.38
98_E102_R1.0250.38
107_G566_V1.0240.38
37_Q526_S1.0240.38
581_Y673_W1.0220.38
49_F711_N1.0220.38
594_I610_E1.0220.38
349_P357_L1.0210.38
89_S116_M1.0200.38
77_V510_L1.0190.38
202_I510_L1.0190.38
241_S408_L1.0190.38
110_E166_S1.0180.38
41_N104_G1.0180.38
180_L184_E1.0180.38
218_V225_S1.0180.38
62_P142_L1.0170.38
69_N157_L1.0170.38
64_H476_L1.0160.38
78_E447_D1.0150.37
80_T91_Y1.0150.37
649_R653_K1.0150.37
364_Y409_E1.0140.37
471_I504_S1.0140.37
112_Y531_S1.0130.37
568_E612_I1.0130.37
220_Y224_S1.0110.37
569_D673_W1.0100.37
622_S645_R1.0100.37
667_C671_K1.0100.37
47_S626_V1.0090.37
121_K518_E1.0090.37
38_L49_F1.0070.37
445_E594_I1.0070.37
391_Q727_I1.0060.37
655_R694_E1.0060.37
657_K688_Q1.0060.37
568_E571_A1.0050.37
668_K713_E1.0050.37
135_E439_L1.0040.37
633_K651_I1.0030.36
81_L89_S1.0020.36
442_T565_K1.0020.36
69_N72_R1.0010.36
51_S358_D1.0010.36
511_Q592_E1.0010.36
168_V380_Q1.0000.36
85_D89_S1.0000.36
703_F721_E1.0000.36
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
2pftA10.70611000.325Contact Map
2b1eA10.67211000.429Contact Map
4y21A1082.50.975Contact Map
3r9vA20.183766.10.978Contact Map
1i84S2053.30.98Contact Map
4rsiB10.153752.30.98Contact Map
3swhA20.319748.40.98Contact Map
4aurA10.115643.60.981Contact Map
4uxvA10.289835.20.982Contact Map
3l39A10.259933.50.982Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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