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gpcr_1e-10

ID: 1501449622 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 292 (285)
Sequences: 18787 (11377.1)
Seq/Len: 65.919
Nf(neff/√len): 673.9

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 65.919).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
13_S55_T4.7611.00
46_H128_W4.2551.00
160_C164_C3.8281.00
193_I197_K3.6031.00
46_H95_M3.0641.00
98_V127_A2.6181.00
196_A200_F2.5571.00
104_S197_K2.5431.00
76_L80_V2.4291.00
190_I194_C2.4131.00
104_S109_R2.3821.00
75_E79_K2.2541.00
197_K200_F2.2071.00
50_A91_A2.2041.00
87_F132_S2.1571.00
118_K122_S2.1021.00
104_S108_T2.0861.00
91_A131_S2.0771.00
232_M290_F2.0431.00
98_V123_M2.0251.00
83_Y87_F1.9811.00
53_L88_S1.9611.00
78_C160_C1.9181.00
71_W74_G1.9081.00
51_N283_D1.9061.00
42_L124_V1.9001.00
95_M124_V1.8891.00
109_R112_A1.8781.00
249_V253_W1.8591.00
18_A22_L1.8491.00
244_W276_A1.8461.00
108_T201_T1.8321.00
46_H124_V1.8311.00
201_T204_R1.8221.00
104_S194_C1.8021.00
197_K201_T1.7821.00
239_S286_I1.7791.00
21_L45_K1.7731.00
200_F204_R1.7111.00
123_M127_A1.7051.00
122_S126_L1.7041.00
102_D106_A1.6591.00
188_L241_T1.6401.00
24_L45_K1.6201.00
97_V194_C1.5911.00
45_K49_L1.5691.00
42_L121_Q1.5491.00
121_Q125_G1.5461.00
43_L120_G1.5381.00
95_M128_W1.5381.00
51_N92_P1.5361.00
101_L194_C1.5341.00
97_V190_I1.5341.00
268_V272_F1.5181.00
83_Y139_L1.5171.00
283_D287_Y1.5151.00
192_L196_A1.4911.00
118_K121_Q1.4771.00
94_F187_P1.4391.00
136_G140_Y1.4381.00
199_I203_T1.4261.00
105_L109_R1.4211.00
54_E280_P1.4181.00
91_A128_W1.4171.00
278_L282_F1.4030.99
50_A92_P1.4000.99
100_S195_N1.3960.99
14_A52_L1.3860.99
48_T51_N1.3820.99
104_S193_I1.3700.99
43_L99_I1.3630.99
74_G77_L1.3620.99
96_M191_M1.3540.99
106_A110_P1.3530.99
91_A95_M1.3160.99
50_A128_W1.3140.99
15_T18_A1.3000.99
116_N119_V1.2930.99
47_L95_M1.2790.99
16_F281_C1.2740.99
178_F182_C1.2730.99
60_P63_G1.2670.99
119_V122_S1.2670.99
199_I230_L1.2590.99
18_A52_L1.2360.98
227_L231_K1.2250.98
101_L105_L1.2230.98
36_K41_K1.2220.98
21_L52_L1.2090.98
43_L95_M1.2080.98
16_F285_L1.1910.98
94_F130_L1.1890.98
106_A113_L1.1830.98
75_E143_R1.1670.97
46_H50_A1.1630.97
90_Y183_L1.1570.97
267_P271_F1.1530.97
100_S194_C1.1490.97
257_D260_M1.1460.97
241_T245_T1.1430.97
245_T249_V1.1420.97
14_A55_T1.1410.97
264_L268_V1.1310.97
97_V191_M1.1290.97
229_T232_M1.1230.97
43_L102_D1.1210.97
20_F24_L1.1200.96
106_A111_L1.1080.96
199_I202_L1.1050.96
21_L48_T1.1040.96
10_F55_T1.1030.96
87_F135_A1.0980.96
205_V209_D1.0980.96
13_S281_C1.0970.96
131_S135_A1.0940.96
19_S23_K1.0940.96
112_A115_S1.0930.96
235_A290_F1.0730.95
273_F277_F1.0690.95
64_M73_A1.0660.95
250_L268_V1.0640.95
43_L98_V1.0630.95
226_R230_L1.0630.95
38_S41_K1.0620.95
250_L272_F1.0590.95
17_N20_F1.0570.95
279_N283_D1.0560.95
41_K45_K1.0440.94
10_F14_A1.0410.94
46_H91_A1.0310.94
108_T197_K1.0270.94
117_S121_Q1.0260.94
39_R120_G1.0190.93
94_F131_S1.0180.93
76_L79_K1.0140.93
93_A187_P1.0120.93
20_F284_P1.0080.93
239_S282_F1.0050.93
138_Q141_I1.0030.93
184_F188_L1.0030.93
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
4iarA10.89381000.087Contact Map
4pxzA10.93151000.098Contact Map
2rh1A10.94521000.098Contact Map
3uonA10.93841000.108Contact Map
3oduA20.94181000.11Contact Map
4dklA10.95211000.111Contact Map
3vw7A10.94521000.113Contact Map
3pblA20.9111000.116Contact Map
4grvA10.96231000.116Contact Map
4bvnA10.92121000.118Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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