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hHsp70

ID: 1501599816 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 641 (616)
Sequences: 5041 (2196.5)
Seq/Len: 8.183
Nf(neff/√len): 88.5

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 8.183).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
141_N168_N5.0211.00
225_D312_S4.6731.00
236_R308_D4.3611.00
209_I320_A3.7821.00
348_K352_D3.6401.00
237_L297_I3.6211.00
279_Q296_S3.5521.00
351_Q358_D3.3351.00
439_L460_E3.1181.00
221_A320_A3.0651.00
400_S412_A3.0391.00
200_L233_F2.9831.00
196_L209_I2.9661.00
242_V254_S2.9141.00
241_F295_T2.8821.00
31_N38_T2.8801.00
198_F313_T2.8581.00
244_E301_R2.7991.00
196_L207_V2.7681.00
141_N170_L2.7671.00
364_N367_E2.6521.00
347_Q361_K2.6511.00
575_Q579_S2.6441.00
412_A415_K2.5261.00
568_K572_D2.4651.00
441_Q455_L2.4331.00
349_L352_D2.3601.00
301_R304_E2.3551.00
340_S343_I2.3461.00
310_F345_K2.3431.00
315_E319_K2.3091.00
530_E534_E2.2781.00
241_F267_C2.2501.00
244_E247_R2.2451.00
307_S311_R2.2241.00
209_I331_I2.1971.00
128_K132_E2.1891.00
69_D231_E2.1471.00
550_K578_I2.1391.00
402_G410_M2.1381.00
163_V168_N2.1251.00
306_C343_I2.0911.00
512_K516_E2.0911.00
302_F343_I2.0861.00
549_M603_C2.0851.00
233_F309_L2.0831.00
517_R521_E2.0701.00
93_Q104_Q2.0701.00
314_L353_F2.0571.00
593_E597_K2.0561.00
523_E526_K2.0271.00
527_A531_V2.0051.00
104_Q113_A1.9841.00
443_Y455_L1.9621.00
595_K598_E1.9531.00
233_F302_F1.9431.00
302_F340_S1.9241.00
298_T301_R1.9211.00
491_T498_A1.9061.00
318_E322_R1.8961.00
564_E568_K1.8951.00
6_A170_L1.8911.00
525_Y528_E1.8701.00
315_E318_E1.8661.00
238_V264_R1.8591.00
394_L419_T1.8431.00
306_C310_F1.8131.00
127_M130_I1.7981.00
573_K576_E1.7961.00
597_K601_Q1.7721.00
321_L328_K1.7651.00
608_S612_Q1.7611.00
129_E133_A1.7441.00
566_D609_G1.7261.00
397_A480_I1.7251.00
145_T373_A1.7221.00
439_L458_R1.7111.00
104_Q111_T1.6811.00
319_K323_D1.6791.00
194_N211_T1.6731.00
412_A448_A1.6661.00
17_C28_I1.6611.00
596_R600_E1.6601.00
304_E307_S1.6581.00
114_F119_I1.6551.00
126_K130_I1.6511.00
246_K252_D1.6431.00
521_E524_K1.6351.00
410_M443_Y1.6341.00
441_Q458_R1.6311.00
211_T220_K1.6261.00
240_H301_R1.6261.00
241_F263_L1.6111.00
520_Q524_K1.6031.00
241_F282_L1.5971.00
6_A377_A1.5961.00
571_L575_Q1.5881.00
62_N65_N1.5871.00
64_Q257_K1.5721.00
64_Q93_Q1.5681.00
572_D576_E1.5681.00
266_A270_A1.5651.00
542_L577_V1.5611.00
303_E348_K1.5401.00
19_G374_A1.5371.00
576_E595_K1.5341.00
200_L205_F1.5241.00
4_A141_N1.5231.00
318_E353_F1.5141.00
7_I18_V1.5121.00
241_F297_I1.5101.00
79_G99_D1.5041.00
348_K351_Q1.5001.00
236_R306_C1.5001.00
603_C607_I1.4991.00
193_R333_D1.4921.00
600_E604_N1.4891.00
327_D330_Q1.4861.00
243_E254_S1.4810.99
79_G96_N1.4800.99
589_K593_E1.4760.99
68_F91_P1.4720.99
538_A593_E1.4700.99
156_Q160_D1.4700.99
49_R122_M1.4610.99
67_V119_I1.4590.99
236_R309_L1.4580.99
342_R366_D1.4580.99
174_N177_T1.4540.99
43_A58_Q1.4520.99
573_K577_V1.4510.99
527_A530_E1.4490.99
144_I162_G1.4430.99
570_V603_C1.4340.99
588_E591_E1.4310.99
526_K530_E1.4300.99
573_K598_E1.4240.99
194_N326_L1.4130.99
244_E293_F1.4120.99
319_K322_R1.4100.99
181_I184_G1.4060.99
58_Q62_N1.4040.99
243_E246_K1.4010.99
559_K567_K1.3990.99
594_H598_E1.3980.99
350_L359_L1.3970.99
70_A123_V1.3950.99
244_E295_T1.3920.99
281_S294_Y1.3920.99
35_N53_D1.3910.99
70_A120_S1.3840.99
235_N239_N1.3830.99
321_L324_A1.3730.99
516_E520_Q1.3700.99
182_A185_L1.3580.99
242_V263_L1.3540.99
345_K348_K1.3430.99
411_T428_F1.3390.99
314_L318_E1.3380.99
307_S345_K1.3380.99
299_R303_E1.3350.99
194_N332_H1.3300.99
120_S158_T1.3280.99
249_H291_I1.3270.99
405_T411_T1.3270.99
70_A119_I1.3270.99
11_L158_T1.3250.99
473_Q493_K1.3220.99
345_K349_L1.3210.99
122_M125_T1.3190.99
44_F114_F1.3190.99
314_L349_L1.3170.99
44_F122_M1.3170.99
17_C37_T1.3170.99
262_R266_A1.3130.98
196_L317_V1.3130.98
605_P608_S1.3110.98
162_G169_V1.3070.98
220_K326_L1.3010.98
239_N254_S1.3000.98
7_I142_A1.2990.98
449_M452_D1.2970.98
239_N243_E1.2950.98
210_L219_V1.2940.98
84_Q87_M1.2930.98
72_R232_D1.2890.98
70_A74_I1.2850.98
318_E328_K1.2840.98
563_S566_D1.2730.98
223_A319_K1.2640.98
604_N607_I1.2590.98
223_A316_P1.2560.98
546_A577_V1.2510.98
62_N66_T1.2500.98
209_I326_L1.2460.98
546_A578_I1.2460.98
317_V350_L1.2390.98
578_I582_D1.2390.98
411_T426_Q1.2360.98
163_V512_K1.2340.97
310_F349_L1.2300.97
351_Q359_L1.2300.97
30_A36_R1.2270.97
467_A492_D1.2250.97
19_G370_A1.2240.97
580_W595_K1.2230.97
326_L330_Q1.2190.97
418_S422_T1.2170.97
160_D163_V1.2160.97
301_R305_L1.2160.97
520_Q523_E1.2140.97
144_I169_V1.2050.97
8_G12_G1.2030.97
511_S514_E1.1940.97
246_K254_S1.1910.97
442_V478_F1.1910.97
78_F84_Q1.1900.97
240_H297_I1.1890.97
548_N552_A1.1880.97
568_K571_L1.1880.97
240_H244_E1.1870.97
392_L482_A1.1850.97
87_M93_Q1.1850.97
541_A596_R1.1830.97
591_E595_K1.1750.96
569_K573_K1.1730.96
546_A574_C1.1710.96
268_E272_R1.1680.96
320_A324_A1.1670.96
97_D102_K1.1660.96
31_N36_R1.1630.96
612_Q616_G1.1600.96
449_M536_V1.1600.96
437_G462_S1.1580.96
13_T203_G1.1530.96
333_D360_N1.1490.96
16_S127_M1.1450.96
36_R367_E1.1440.96
535_R539_K1.1390.95
253_I287_L1.1320.95
194_N220_K1.1310.95
455_L458_R1.1300.95
567_K571_L1.1290.95
323_D416_R1.1280.95
237_L240_H1.1270.95
176_P206_D1.1250.95
437_G460_E1.1240.95
76_R80_D1.1230.95
596_R599_L1.1210.95
514_E517_R1.1210.95
22_Q27_E1.1140.95
594_H597_K1.1100.95
148_A206_D1.1090.94
242_V245_F1.1060.94
237_L267_C1.1060.94
579_S582_D1.0990.94
528_E531_V1.0970.94
282_L297_I1.0930.94
129_E132_E1.0930.94
565_A569_K1.0910.94
16_S40_S1.0890.94
579_S583_A1.0860.94
181_I186_D1.0840.94
11_L120_S1.0820.94
545_Y549_M1.0810.93
20_V135_L1.0800.93
242_V260_V1.0800.93
553_V571_L1.0790.93
270_A280_A1.0760.93
425_T477_T1.0720.93
235_N238_V1.0710.93
262_R284_I1.0680.93
19_G28_I1.0680.93
233_F343_I1.0660.93
70_A103_V1.0610.93
270_A297_I1.0580.92
554_E571_L1.0570.92
114_F122_M1.0560.92
233_F310_F1.0540.92
425_T475_E1.0530.92
170_L380_L1.0530.92
314_L317_V1.0530.92
376_Q379_I1.0490.92
533_R537_S1.0490.92
132_E138_P1.0480.92
183_Y186_D1.0440.92
545_Y603_C1.0440.92
95_I113_A1.0430.92
118_E122_M1.0350.91
223_A323_D1.0320.91
605_P609_G1.0310.91
100_K117_E1.0290.91
465_P494_S1.0260.91
542_L592_F1.0240.91
11_L124_L1.0220.91
603_C606_I1.0200.91
144_I158_T1.0170.90
9_I12_G1.0150.90
18_V127_M1.0140.90
121_S164_I1.0120.90
182_A186_D1.0110.90
184_G187_R1.0100.90
443_Y450_T1.0100.90
569_K572_D1.0090.90
84_Q88_K1.0090.90
200_L313_T1.0080.90
225_D228_L1.0080.90
306_C345_K1.0080.90
71_K175_E1.0050.90
125_T129_E1.0010.89
543_E547_F1.0000.89
200_L346_V1.0000.89
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
3d2fA20.95321000.217Contact Map
4fl9A10.84091000.269Contact Map
4jneA20.9221000.27Contact Map
2v7yA10.781000.347Contact Map
4rtfD10.53821000.36Contact Map
3i33A10.58971000.511Contact Map
4j8fA10.84561000.532Contact Map
4kboA10.57721000.544Contact Map
3qfuA10.58971000.575Contact Map
4gniA20.58191000.576Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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