GREMLIN.BAKERLAB.org
Powered by OPENSEQ.org
hHsp70

ID: 1501601455 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 641 (627)
Sequences: 8390 (4180.3)
Seq/Len: 13.381
Nf(neff/√len): 166.9

Jackhmmer Results: (2016_04)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 13.381).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
141_N168_N4.8611.00
225_D312_S4.7011.00
236_R308_D4.2661.00
351_Q358_D3.7401.00
69_D231_E3.4291.00
348_K352_D3.4051.00
237_L297_I3.3791.00
279_Q296_S3.3651.00
198_F313_T3.3561.00
31_N38_T3.1961.00
209_I320_A3.1791.00
400_S412_A3.0851.00
242_V254_S2.9481.00
441_Q455_L2.8951.00
364_N367_E2.7951.00
439_L460_E2.7411.00
241_F295_T2.7331.00
568_K572_D2.7181.00
550_K578_I2.7181.00
244_E301_R2.7171.00
141_N170_L2.6941.00
200_L233_F2.6691.00
512_K516_E2.6121.00
411_T428_F2.5411.00
221_A320_A2.5171.00
412_A415_K2.5041.00
575_Q579_S2.4701.00
35_N53_D2.4511.00
79_G96_N2.4151.00
196_L209_I2.3451.00
347_Q361_K2.3341.00
64_Q93_Q2.3341.00
6_A170_L2.3331.00
307_S311_R2.3301.00
196_L207_V2.3261.00
525_Y528_E2.3071.00
301_R304_E2.2691.00
310_F345_K2.2641.00
402_G410_M2.1981.00
608_S612_Q2.1861.00
517_R521_E2.1831.00
579_S582_D2.1801.00
302_F343_I2.1761.00
340_S343_I2.1681.00
588_E591_E2.1461.00
349_L352_D2.1411.00
559_K567_K2.1351.00
7_I18_V2.1161.00
523_E526_K2.1121.00
128_K132_E2.1111.00
491_T498_A2.0481.00
530_E534_E2.0311.00
235_N239_N2.0231.00
163_V168_N2.0201.00
22_Q27_E2.0091.00
315_E319_K2.0031.00
589_K593_E2.0021.00
315_E318_E2.0001.00
306_C343_I1.9991.00
233_F309_L1.9951.00
314_L353_F1.9931.00
443_Y455_L1.9771.00
281_S294_Y1.9621.00
593_E597_K1.9601.00
49_R122_M1.9581.00
563_S566_D1.9541.00
244_E247_R1.9151.00
241_F267_C1.9111.00
318_E322_R1.8581.00
306_C310_F1.8421.00
527_A531_V1.8371.00
597_K601_Q1.8311.00
145_T373_A1.8241.00
129_E133_A1.8221.00
566_D609_G1.8171.00
238_V264_R1.8171.00
571_L575_Q1.8101.00
19_G374_A1.8101.00
572_D576_E1.7811.00
84_Q87_M1.7761.00
410_M443_Y1.7641.00
520_Q523_E1.7601.00
114_F119_I1.7401.00
6_A377_A1.7361.00
394_L419_T1.7301.00
564_E568_K1.7241.00
516_E520_Q1.7241.00
64_Q257_K1.7201.00
542_L577_V1.7161.00
520_Q524_K1.7101.00
327_D330_Q1.7091.00
605_P608_S1.6991.00
249_H291_I1.6991.00
117_E160_D1.6971.00
314_L318_E1.6861.00
318_E353_F1.6841.00
266_A270_A1.6811.00
302_F340_S1.6621.00
193_R333_D1.6621.00
595_K598_E1.6561.00
439_L458_R1.6511.00
163_V512_K1.6481.00
576_E595_K1.6431.00
573_K576_E1.6431.00
549_M603_C1.6361.00
65_N110_E1.6151.00
44_F122_M1.6111.00
268_E272_R1.6081.00
441_Q458_R1.6061.00
17_C37_T1.5901.00
303_E348_K1.5901.00
156_Q160_D1.5751.00
270_A282_L1.5671.00
567_K571_L1.5641.00
319_K323_D1.5591.00
17_C28_I1.5571.00
342_R366_D1.5551.00
243_E254_S1.5521.00
209_I326_L1.5501.00
241_F263_L1.5471.00
397_A480_I1.5401.00
126_K130_I1.5401.00
573_K598_E1.5391.00
304_E307_S1.5261.00
321_L328_K1.5211.00
412_A448_A1.5171.00
36_R367_E1.5151.00
127_M130_I1.5101.00
546_A578_I1.4981.00
298_T301_R1.4961.00
600_E604_N1.4941.00
246_K252_D1.4931.00
104_Q111_T1.4921.00
270_A280_A1.4891.00
333_D360_N1.4871.00
590_D594_H1.4801.00
455_L458_R1.4781.00
449_M536_V1.4771.00
604_N607_I1.4761.00
585_T588_E1.4761.00
564_E571_L1.4721.00
240_H301_R1.4711.00
301_R305_L1.4711.00
43_A58_Q1.4591.00
253_I287_L1.4571.00
418_S422_T1.4521.00
233_F302_F1.4511.00
405_T411_T1.4481.00
449_M452_D1.4471.00
223_A316_P1.4441.00
594_H598_E1.4441.00
241_F282_L1.4381.00
345_K349_L1.4271.00
122_M125_T1.4251.00
96_N99_D1.4141.00
42_V119_I1.4081.00
348_K351_Q1.4020.99
603_C607_I1.4020.99
473_Q493_K1.3980.99
511_S514_E1.3980.99
546_A577_V1.3970.99
527_A530_E1.3960.99
194_N326_L1.3930.99
345_K348_K1.3920.99
239_N243_E1.3920.99
104_Q113_A1.3880.99
29_I134_Y1.3840.99
19_G370_A1.3840.99
209_I331_I1.3800.99
68_F91_P1.3700.99
514_E517_R1.3550.99
546_A574_C1.3520.99
194_N220_K1.3460.99
240_H244_E1.3390.99
11_L158_T1.3380.99
579_S583_A1.3350.99
425_T475_E1.3340.99
427_I475_E1.3260.99
319_K322_R1.3180.99
16_S127_M1.3180.99
314_L349_L1.3160.99
223_A319_K1.3120.99
244_E295_T1.3100.99
236_R306_C1.3080.99
21_F378_A1.3070.99
70_A119_I1.2970.99
200_L205_F1.2970.99
578_I582_D1.2960.99
144_I162_G1.2960.99
318_E328_K1.2950.99
538_A593_E1.2900.99
404_E441_Q1.2890.99
240_H295_T1.2890.99
160_D163_V1.2870.99
120_S158_T1.2870.99
310_F349_L1.2850.99
591_E595_K1.2820.99
128_K167_L1.2790.99
425_T477_T1.2760.99
44_F114_F1.2720.99
350_L359_L1.2680.99
159_K485_I1.2650.99
30_A134_Y1.2620.99
18_V127_M1.2610.99
607_I611_Y1.2610.99
588_E592_F1.2580.99
609_G612_Q1.2520.99
62_N65_N1.2480.99
70_A120_S1.2460.98
118_E122_M1.2430.98
162_G172_I1.2430.98
307_S345_K1.2410.98
220_K326_L1.2330.98
32_D130_I1.2310.98
266_A269_R1.2300.98
239_N254_S1.2290.98
299_R303_E1.2220.98
70_A123_V1.2220.98
237_L267_C1.2160.98
521_E524_K1.2160.98
528_E531_V1.2150.98
240_H297_I1.2090.98
566_D569_K1.2040.98
565_A569_K1.2010.98
594_H597_K1.2000.98
534_E589_K1.1960.98
28_I371_Y1.1930.98
518_M521_E1.1930.98
93_Q104_Q1.1920.98
86_D227_H1.1890.98
233_F306_C1.1860.98
95_I113_A1.1850.98
174_N177_T1.1850.98
569_K573_K1.1830.98
270_A297_I1.1790.98
194_N211_T1.1780.98
170_L380_L1.1720.98
196_L317_V1.1650.97
411_T426_Q1.1640.97
240_H305_L1.1630.97
282_L297_I1.1620.97
596_R599_L1.1550.97
58_Q62_N1.1550.97
423_K477_T1.1520.97
283_E294_Y1.1520.97
538_A541_A1.1510.97
538_A596_R1.1450.97
323_D416_R1.1440.97
30_A36_R1.1420.97
541_A596_R1.1400.97
320_A324_A1.1370.97
244_E293_F1.1360.97
573_K577_V1.1350.97
596_R600_E1.1300.97
592_F596_R1.1290.97
437_G460_E1.1250.97
465_P494_S1.1250.97
87_M94_V1.1220.97
592_F599_L1.1220.97
443_Y450_T1.1210.97
67_V119_I1.1190.96
84_Q88_K1.1180.96
211_T220_K1.1170.96
238_V267_C1.1170.96
125_T129_E1.1140.96
311_R349_L1.1140.96
569_K602_V1.1110.96
556_E559_K1.1060.96
526_K530_E1.1050.96
41_Y68_F1.1010.96
97_D102_K1.0960.96
233_F310_F1.0890.96
535_R539_K1.0880.96
604_N608_S1.0840.96
516_E519_V1.0810.95
16_S40_S1.0780.95
162_G169_V1.0780.95
79_G99_D1.0760.95
549_M574_C1.0760.95
233_F343_I1.0740.95
589_K592_F1.0740.95
437_G462_S1.0710.95
242_V260_V1.0700.95
605_P609_G1.0690.95
7_I139_V1.0680.95
242_V263_L1.0630.95
223_A323_D1.0620.95
533_R537_S1.0570.95
321_L326_L1.0530.95
43_A50_L1.0510.94
568_K571_L1.0480.94
13_T203_G1.0460.94
11_L120_S1.0450.94
591_E594_H1.0350.94
590_D593_E1.0340.94
87_M93_Q1.0310.94
45_T48_E1.0300.94
236_R309_L1.0290.94
246_K254_S1.0250.93
132_E138_P1.0230.93
326_L330_Q1.0210.93
70_A74_I1.0180.93
235_N238_V1.0180.93
174_N395_D1.0170.93
562_I567_K1.0170.93
266_A280_A1.0150.93
607_I610_L1.0110.93
360_N368_A1.0050.93
306_C309_L1.0050.93
210_L219_V1.0050.93
20_V135_L1.0050.93
545_Y549_M1.0050.93
573_K602_V1.0030.93
554_E559_K1.0000.92
494_S611_Y1.0000.92
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
3d2fA20.95631000.166Contact Map
4jneA20.9221000.228Contact Map
4fl9A10.84091000.231Contact Map
2v7yA10.781000.315Contact Map
4rtfD10.53821000.328Contact Map
3i33A10.58971000.471Contact Map
4j8fA10.85181000.487Contact Map
4kboA10.57721000.507Contact Map
4gniA20.58191000.539Contact Map
3qfuA10.58971000.54Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0574 seconds.