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MalT180717 1-800

ID: 1501608556 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 800 (775)
Sequences: 5153 (4031.8)
Seq/Len: 6.649
Nf(neff/√len): 144.8

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 6.649).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
585_R588_E6.6151.00
543_F546_T6.3301.00
624_D627_N5.5851.00
665_D668_A5.3301.00
49_I158_L4.9311.00
344_H363_H4.8641.00
424_R427_E4.8511.00
464_N467_E4.5731.00
743_R746_D4.4361.00
503_E506_R4.3091.00
703_E706_P4.1501.00
548_W552_E3.2501.00
191_A222_L3.2401.00
535_S551_Q3.1721.00
187_T190_E3.1141.00
195_F226_A2.8021.00
590_E622_R2.7771.00
22_L48_L2.6891.00
27_S55_G2.6841.00
188_H209_S2.6831.00
495_G511_M2.5911.00
53_A126_V2.5741.00
512_Q534_Q2.5411.00
37_I49_I2.5081.00
130_Y157_V2.4921.00
26_L35_A2.4661.00
352_M372_M2.4541.00
548_W583_W2.4301.00
712_E734_L2.3831.00
719_R753_D2.3761.00
39_S43_Y2.3751.00
538_L543_F2.3471.00
274_S320_Y2.3371.00
508_L512_Q2.3041.00
191_A212_C2.2441.00
26_L52_W2.2331.00
47_T51_Q2.2131.00
41_A160_R2.2061.00
508_L541_Q2.0991.00
325_G329_R2.0941.00
548_W576_R2.0941.00
51_Q54_A2.0711.00
670_A674_R2.0701.00
616_I632_L2.0111.00
735_N751_L2.0041.00
590_E594_R2.0001.00
538_L546_T1.9981.00
552_E576_R1.9961.00
49_I126_V1.9771.00
629_R663_T1.9631.00
264_D300_E1.9591.00
580_L588_E1.9521.00
480_P513_Q1.9231.00
508_L534_Q1.9041.00
580_L585_R1.8701.00
415_Q419_M1.8631.00
123_L153_L1.8341.00
204_E207_E1.8281.00
508_L538_L1.8141.00
130_Y139_H1.8091.00
348_A364_A1.7981.00
27_S52_W1.7961.00
416_A435_A1.7961.00
267_T300_E1.7911.00
49_I53_A1.7901.00
16_T47_T1.7841.00
619_S627_N1.7821.00
734_L750_V1.7581.00
268_R272_L1.7541.00
531_L553_K1.7501.00
629_R633_N1.7411.00
590_E615_L1.7351.00
275_A329_R1.7181.00
498_L506_R1.7181.00
708_E712_E1.7101.00
277_L347_A1.6971.00
345_R369_D1.6891.00
429_N433_A1.6591.00
577_A593_A1.6561.00
193_Q197_C1.6451.00
42_G45_K1.6381.00
748_Q752_L1.6311.00
345_R349_E1.6281.00
559_N595_S1.6251.00
738_Y746_D1.6211.00
75_A78_L1.6061.00
734_L738_Y1.6051.00
288_V350_S1.5991.00
491_T510_L1.5911.00
188_H217_G1.5801.00
534_Q550_T1.5801.00
507_S537_I1.5721.00
469_E473_K1.5711.00
519_R553_K1.5670.99
51_Q55_G1.5640.99
580_L592_S1.5630.99
271_L328_L1.5610.99
128_D158_L1.5470.99
211_I222_L1.5470.99
18_V23_L1.5450.99
618_C622_R1.5400.99
270_F297_R1.5380.99
419_M427_E1.5310.99
518_A526_Y1.5280.99
660_W665_D1.5230.99
125_L155_L1.5230.99
708_E737_L1.5220.99
619_S631_Q1.5190.99
531_L550_T1.5060.99
619_S624_D1.5040.99
588_E591_A1.4980.99
731_L753_D1.4970.99
53_A124_Y1.4890.99
49_I156_V1.4840.99
535_S554_A1.4830.99
224_L259_V1.4750.99
356_F359_E1.4700.99
37_I158_L1.4660.99
416_A432_L1.4650.99
708_E741_A1.4640.99
419_M431_L1.4590.99
738_Y743_R1.4580.99
307_F324_F1.4570.99
759_N767_F1.4500.99
576_R580_L1.4450.99
660_W668_A1.4400.99
538_L550_T1.4380.99
188_H213_D1.4350.99
264_D267_T1.4310.99
192_K208_S1.4300.99
35_A154_T1.4280.99
160_R321_H1.4180.99
731_L750_V1.4120.99
369_D372_M1.4080.99
171_R178_E1.4000.99
552_E588_E1.3970.99
191_A217_G1.3950.99
506_R509_A1.3910.99
58_D121_S1.3900.98
495_G514_T1.3880.98
186_F194_F1.3870.98
528_L554_A1.3870.98
263_V267_T1.3860.98
25_K29_A1.3790.98
508_L537_I1.3790.98
436_E455_R1.3720.98
134_T138_I1.3680.98
552_E556_Q1.3660.98
612_L631_Q1.3490.98
594_R615_L1.3370.98
39_S44_G1.3250.98
587_D626_D1.3210.98
715_N734_L1.3190.98
544_L625_L1.3170.98
514_T530_S1.3130.98
342_E346_A1.3040.97
37_I48_L1.3000.97
39_S45_K1.3000.97
698_Q703_E1.2970.97
511_M593_A1.2950.97
599_V638_L1.2950.97
548_W585_R1.2940.97
735_N754_A1.2940.97
587_D622_R1.2940.97
498_L619_S1.2910.97
615_L631_Q1.2910.97
765_S769_I1.2860.97
477_E516_Q1.2840.97
628_A635_L1.2840.97
149_Q153_L1.2840.97
626_D663_T1.2830.97
216_S322_P1.2770.97
588_E592_S1.2770.97
697_A701_L1.2760.97
520_Q641_N1.2730.97
573_V592_S1.2690.97
708_E743_R1.2620.97
43_Y219_A1.2570.97
543_F703_E1.2570.97
516_Q556_Q1.2560.97
209_S213_D1.2540.97
715_N727_L1.2500.97
551_Q575_I1.2490.96
194_F222_L1.2490.96
389_E392_L1.2490.96
708_E734_L1.2470.96
22_L190_E1.2470.96
747_A751_L1.2430.96
537_I541_Q1.2420.96
513_Q553_K1.2380.96
548_W580_L1.2340.96
549_E591_A1.2340.96
36_L159_S1.2320.96
502_G542_G1.2300.96
39_S42_G1.2290.96
670_A701_L1.2280.96
738_Y750_V1.2280.96
715_N730_N1.2240.96
270_F294_G1.2220.96
515_E534_Q1.2210.96
128_D131_H1.2200.96
590_E619_S1.2200.96
19_R22_L1.2200.96
42_G46_T1.2190.96
549_E671_N1.2170.96
469_E494_L1.2170.96
43_Y218_W1.2160.96
553_K634_R1.2160.96
433_A437_H1.2100.96
265_L268_R1.2080.96
668_A671_N1.2050.95
272_L331_R1.2010.95
473_K477_E1.1960.95
531_L557_L1.1910.95
590_E618_C1.1890.95
210_R213_D1.1870.95
216_S319_C1.1840.95
698_Q706_P1.1820.95
393_L396_S1.1790.95
573_V595_S1.1780.95
719_R727_L1.1760.95
210_R214_D1.1740.95
146_I155_L1.1730.95
29_A35_A1.1650.94
186_F190_E1.1640.94
713_E717_N1.1630.94
284_L350_S1.1620.94
541_Q701_L1.1620.94
577_A589_A1.1600.94
546_T627_N1.1580.94
465_P544_L1.1580.94
519_R527_A1.1580.94
270_F285_I1.1550.94
594_R633_N1.1540.94
550_T592_S1.1510.94
286_T294_G1.1500.94
16_T19_R1.1450.94
633_N674_R1.1440.94
483_W644_Y1.1420.93
16_T51_Q1.1420.93
44_G48_L1.1420.93
509_A630_S1.1420.93
555_F576_R1.1420.93
470_R509_A1.1400.93
195_F208_S1.1390.93
498_L510_L1.1340.93
465_P504_L1.1320.93
467_E546_T1.1300.93
708_E738_Y1.1270.93
548_W579_L1.1270.93
491_T513_Q1.1250.93
535_S547_A1.1240.93
667_A705_E1.1180.92
192_K205_A1.1180.92
280_M285_I1.1160.92
476_L555_F1.1150.92
699_I737_L1.1150.92
288_V347_A1.1150.92
556_Q637_N1.1140.92
47_T223_Q1.1130.92
46_T50_S1.1090.92
589_A618_C1.1090.92
504_L537_I1.1060.92
601_S608_Q1.1040.92
511_M537_I1.1020.92
38_T159_S1.1020.92
636_E656_R1.1010.92
20_E24_A1.1010.92
612_L634_R1.0950.91
267_T270_F1.0940.91
728_N757_L1.0890.91
456_A472_A1.0880.91
21_R25_K1.0880.91
474_L595_S1.0850.91
707_A737_L1.0850.91
616_I628_A1.0840.91
364_A372_M1.0830.91
215_V222_L1.0830.91
532_I575_I1.0830.91
506_R510_L1.0810.91
272_L339_E1.0800.91
455_R459_A1.0790.91
462_D622_R1.0780.91
329_R340_L1.0780.91
534_Q538_L1.0750.90
474_L477_E1.0740.90
590_E624_D1.0730.90
494_L498_L1.0710.90
671_N709_I1.0700.90
513_Q634_R1.0700.90
544_L579_L1.0650.90
575_I614_M1.0640.90
756_K760_R1.0630.90
544_L586_L1.0620.90
586_L625_L1.0620.90
516_Q598_E1.0600.90
248_A251_L1.0600.90
724_M727_L1.0600.90
511_M551_Q1.0580.89
699_I733_L1.0580.89
758_A761_T1.0570.89
593_A632_L1.0570.89
627_N631_Q1.0560.89
470_R549_E1.0550.89
198_R223_Q1.0530.89
505_T541_Q1.0520.89
473_K552_E1.0490.89
190_E193_Q1.0480.89
579_L583_W1.0480.89
129_D132_L1.0470.89
518_A530_S1.0450.89
553_K556_Q1.0420.89
544_L666_K1.0390.88
130_Y133_I1.0380.88
224_L227_L1.0380.88
470_R630_S1.0360.88
547_A579_L1.0360.88
340_L344_H1.0350.88
549_E553_K1.0310.88
576_R592_S1.0310.88
706_P709_I1.0310.88
144_F148_H1.0310.88
45_K48_L1.0290.88
23_L51_Q1.0270.88
392_L396_S1.0260.87
547_A554_A1.0240.87
714_L730_N1.0220.87
634_R675_H1.0220.87
591_A671_N1.0210.87
52_W124_Y1.0180.87
577_A615_L1.0180.87
478_E517_M1.0170.87
25_K183_Q1.0150.87
467_E470_R1.0150.87
50_S61_W1.0140.87
771_G775_A1.0130.87
367_A373_L1.0130.87
555_F569_H1.0110.86
434_R513_Q1.0100.86
6_K77_Y1.0090.86
40_P43_Y1.0080.86
160_R323_L1.0080.86
554_A635_L1.0080.86
698_Q738_Y1.0070.86
735_N747_A1.0060.86
287_R353_A1.0060.86
360_A364_A1.0040.86
511_M534_Q1.0010.86
626_D705_E1.0010.86
545_Q667_A1.0010.86
505_T545_Q1.0000.86
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
1z6tA40.53371000.673Contact Map
1hz4A10.45371000.705Contact Map
2a5yB20.43251000.724Contact Map
3sf4A30.42251000.756Contact Map
4ui9O101000.762Contact Map
2qenA10.391000.765Contact Map
4a1sA20.38751000.768Contact Map
3ro2A10.386299.90.771Contact Map
1w3bA20.483899.90.773Contact Map
2xpiA20.56599.90.774Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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