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gpcr_1e-40

ID: 1501613380 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 292 (282)
Sequences: 7252 (4014.7)
Seq/Len: 25.716
Nf(neff/√len): 239.1

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 25.716).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
13_S55_T5.2301.00
76_L80_V3.8071.00
193_I197_K3.6021.00
104_S109_R2.9311.00
46_H128_W2.8371.00
98_V127_A2.8231.00
75_E79_K2.8121.00
46_H95_M2.7171.00
71_W74_G2.5881.00
18_A22_L2.4921.00
21_L45_K2.3281.00
83_Y87_F2.2961.00
98_V123_M2.1811.00
239_S286_I2.1471.00
197_K200_F2.0811.00
196_A200_F2.0261.00
244_W276_A2.0181.00
24_L45_K2.0081.00
104_S197_K1.9311.00
87_F132_S1.9271.00
64_M73_A1.9211.00
42_L124_V1.9061.00
200_F204_R1.8301.00
190_I194_C1.7751.00
267_P271_F1.7601.00
75_E143_R1.7531.00
43_L120_G1.7491.00
101_L194_C1.7151.00
232_M290_F1.7071.00
83_Y139_L1.7031.00
95_M124_V1.6871.00
48_T51_N1.6821.00
180_F185_I1.6681.00
102_D106_A1.6391.00
136_G140_Y1.6281.00
247_Y251_G1.6171.00
21_L52_L1.5951.00
250_L268_V1.5811.00
91_A131_S1.5631.00
180_F252_I1.5541.00
283_D287_Y1.5451.00
36_K41_K1.5361.00
123_M127_A1.5351.00
188_L241_T1.5341.00
250_L272_F1.5271.00
178_F182_C1.5191.00
144_M158_S1.5051.00
38_S41_K1.4991.00
280_P283_D1.4971.00
192_L196_A1.4771.00
21_L24_L1.4691.00
118_K122_S1.4631.00
122_S126_L1.4421.00
121_Q125_G1.4361.00
83_Y132_S1.4251.00
106_A110_P1.4131.00
184_F245_T1.3900.99
199_I230_L1.3890.99
244_W283_D1.3790.99
229_T232_M1.3630.99
10_F60_P1.3610.99
197_K201_T1.3490.99
14_A55_T1.3460.99
54_E92_P1.3420.99
20_F288_G1.3370.99
249_V253_W1.3340.99
96_M191_M1.3340.99
146_H158_S1.3310.99
104_S108_T1.3200.99
191_M237_A1.3120.99
264_L268_V1.3080.99
18_A52_L1.3070.99
177_F256_F1.2980.99
245_T249_V1.2900.99
74_G77_L1.2780.99
241_T245_T1.2750.99
94_F97_V1.2690.99
247_Y276_A1.2620.99
184_F241_T1.2560.99
50_A128_W1.2480.99
90_Y183_L1.2480.99
51_N283_D1.2470.99
45_K49_L1.2380.98
20_F287_Y1.2380.98
93_A97_V1.2360.98
93_A240_F1.2320.98
235_A286_I1.2210.98
126_L130_L1.2190.98
268_V272_F1.2110.98
10_F59_M1.2040.98
104_S193_I1.1870.98
199_I203_T1.1780.98
61_L77_L1.1780.98
191_M195_N1.1720.98
109_R112_A1.1640.97
15_T18_A1.1640.97
53_L88_S1.1610.97
46_H124_V1.1530.97
7_F63_G1.1490.97
13_S281_C1.1360.97
12_L15_T1.1340.97
10_F63_G1.1320.97
102_D228_K1.1200.96
63_G67_I1.1180.96
191_M194_C1.1170.96
234_V238_T1.1070.96
64_M72_Y1.0980.96
16_F20_F1.0950.96
99_I233_T1.0940.96
136_G139_L1.0830.96
122_S125_G1.0800.95
91_A95_M1.0790.95
251_G255_W1.0730.95
78_C160_C1.0730.95
74_G79_K1.0720.95
288_G291_S1.0700.95
7_F67_I1.0700.95
74_G78_C1.0690.95
47_L51_N1.0680.95
43_L99_I1.0610.95
93_A248_Y1.0600.95
94_F130_L1.0600.95
91_A128_W1.0590.95
283_D291_S1.0590.95
21_L49_L1.0540.95
73_A77_L1.0520.95
202_L229_T1.0480.94
280_P284_P1.0340.94
239_S282_F1.0250.93
12_L285_L1.0240.93
26_K33_K1.0240.93
14_A52_L1.0230.93
86_L248_Y1.0210.93
21_L48_T1.0040.93
39_R43_L1.0010.92
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
4iarA10.89381000.162Contact Map
4bvnA10.91781000.194Contact Map
4pxzA10.93151000.194Contact Map
2rh1A10.94181000.195Contact Map
3uonA10.93841000.197Contact Map
3oduA20.94181000.205Contact Map
3pblA20.9111000.207Contact Map
3vw7A10.94521000.21Contact Map
4dklA10.95211000.211Contact Map
3rzeA10.91441000.213Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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