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ImpBeta

ID: 1501840019 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 876 (866)
Sequences: 1373 (749.3)
Seq/Len: 1.585
Nf(neff/√len): 25.5

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.585).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
383_R419_D2.4511.00
453_L474_F2.2280.99
128_E132_Q2.1750.99
41_S94_Y1.8480.97
605_D645_K1.8230.96
92_K126_W1.7560.95
212_E216_H1.6780.94
99_L133_L1.6740.93
383_R417_M1.6600.93
654_L674_V1.6510.93
47_P101_T1.6380.92
837_V869_L1.6310.92
19_E23_K1.6120.92
26_E29_A1.5880.91
507_V532_L1.5850.91
33_L36_F1.5460.89
93_N97_Q1.5430.89
99_L190_V1.5180.88
56_A60_Q1.5140.88
834_L872_L1.4970.87
5_T12_S1.4950.87
857_K860_T1.4870.87
22_Q26_E1.4840.86
693_M717_F1.4810.86
33_L37_L1.4800.86
833_V874_N1.4790.86
23_K27_R1.4710.86
92_K120_E1.4620.85
81_L125_Q1.4610.85
131_P172_E1.4590.85
797_I821_L1.4580.85
771_G775_G1.4570.85
776_L779_D1.4520.85
855_T858_A1.4480.85
469_N627_D1.4480.85
674_V677_L1.4470.85
496_T500_S1.4450.84
500_S542_D1.4400.84
847_L859_K1.4360.84
721_A728_F1.4300.84
818_A822_I1.4280.83
843_I851_R1.4250.83
844_H848_T1.4200.83
657_G670_A1.4150.83
496_T542_D1.4120.83
643_F646_Y1.4080.82
364_V405_L1.4070.82
95_V822_I1.3990.82
136_N150_T1.3980.82
841_P845_E1.3970.82
633_S636_V1.3910.81
18_L22_Q1.3840.81
390_F409_A1.3830.81
870_R873_K1.3780.80
844_H871_K1.3750.80
863_T866_T1.3750.80
202_L247_L1.3700.80
450_A454_Q1.3680.80
807_D811_T1.3680.80
5_T9_K1.3640.79
218_I584_L1.3530.79
419_D424_V1.3490.78
293_L297_A1.3400.78
700_L708_S1.3360.77
868_E871_K1.3340.77
62_K112_C1.3310.77
23_K26_E1.3280.77
166_L173_I1.3270.77
126_W159_Q1.3250.77
591_V595_V1.3240.77
690_D731_Y1.3230.76
194_A241_L1.3220.76
834_L838_E1.3220.76
250_Q572_S1.3170.76
256_M678_C1.3080.75
326_L348_A1.3080.75
511_L557_R1.3050.75
856_N860_T1.3050.75
3_L28_A1.3030.75
244_I677_L1.3030.75
632_V654_L1.2980.75
25_L28_A1.2970.74
352_L389_A1.2970.74
379_D382_Y1.2970.74
797_I807_D1.2960.74
845_E851_R1.2950.74
833_V841_P1.2940.74
845_E848_T1.2940.74
38_V87_A1.2910.74
453_L502_S1.2890.74
834_L867_K1.2860.74
840_R844_H1.2830.73
860_T872_L1.2780.73
764_S805_A1.2770.73
3_L8_E1.2770.73
391_G430_W1.2760.73
558_L660_N1.2710.72
16_L27_R1.2700.72
364_V402_L1.2670.72
63_N324_P1.2620.72
210_D835_K1.2610.71
861_L870_R1.2600.71
833_V837_V1.2590.71
779_D850_G1.2560.71
117_A129_L1.2520.71
869_L872_L1.2520.71
181_M195_T1.2450.70
832_D856_N1.2450.70
65_L147_K1.2440.70
848_T866_T1.2440.70
57_A126_W1.2420.70
833_V871_K1.2410.70
672_G676_D1.2380.69
61_I434_R1.2360.69
850_G861_L1.2350.69
6_I9_K1.2310.69
133_L154_I1.2260.68
571_T595_V1.2260.68
845_E856_N1.2220.68
16_L28_A1.2210.68
99_L129_L1.2200.68
276_A384_D1.2200.68
858_A861_L1.2190.68
493_E852_R1.2180.68
868_E872_L1.2170.68
18_L21_A1.2170.68
863_T867_K1.2160.67
848_T863_T1.2130.67
836_L840_R1.2130.67
848_T867_K1.2110.67
578_N591_V1.2090.67
760_E813_G1.2090.67
126_W151_L1.2070.67
871_K874_N1.2060.67
99_L113_V1.2040.66
409_A413_L1.2040.66
841_P863_T1.2020.66
16_L22_Q1.1970.66
766_L818_A1.1970.66
845_E849_E1.1940.65
291_M357_T1.1940.65
861_L867_K1.1920.65
255_Y264_T1.1910.65
384_D736_L1.1910.65
805_A818_A1.1900.65
181_M562_L1.1890.65
802_D806_G1.1880.65
8_E16_L1.1880.65
766_L804_I1.1880.65
778_G841_P1.1860.65
95_V112_C1.1850.65
717_F818_A1.1820.64
850_G863_T1.1820.64
5_T23_K1.1810.64
16_L20_A1.1800.64
850_G866_T1.1800.64
44_L58_G1.1790.64
866_T870_R1.1790.64
837_V872_L1.1770.64
840_R845_E1.1770.64
88_R92_K1.1750.64
13_P23_K1.1750.64
611_L631_A1.1750.64
24_F30_V1.1740.63
96_L129_L1.1730.63
790_V869_L1.1730.63
11_V18_L1.1720.63
837_V868_E1.1720.63
849_E860_T1.1720.63
833_V849_E1.1710.63
845_E855_T1.1700.63
773_V794_V1.1680.63
859_K869_L1.1650.63
802_D817_C1.1650.63
856_N867_K1.1650.63
36_F60_Q1.1640.63
390_F713_I1.1640.63
533_M590_N1.1620.62
851_R866_T1.1620.62
845_E863_T1.1580.62
151_L771_G1.1570.62
868_E873_K1.1570.62
394_L434_R1.1570.62
833_V838_E1.1560.62
8_E26_E1.1550.62
835_K838_E1.1520.61
838_E873_K1.1510.61
311_S547_V1.1490.61
839_A848_T1.1490.61
222_V743_S1.1480.61
800_F804_I1.1480.61
831_K836_L1.1470.61
364_V804_I1.1460.61
852_R870_R1.1450.61
832_D857_K1.1430.60
852_R857_K1.1430.60
832_D859_K1.1410.60
4_I53_A1.1410.60
525_R689_C1.1390.60
206_K210_D1.1370.60
851_R856_N1.1370.60
272_I650_F1.1350.60
751_D754_M1.1350.60
846_L857_K1.1350.60
26_E30_V1.1340.60
70_P74_A1.1330.59
736_L793_R1.1330.59
91_V302_E1.1320.59
853_S859_K1.1320.59
380_W718_G1.1310.59
91_V814_V1.1300.59
848_T872_L1.1290.59
858_A863_T1.1280.59
226_T720_I1.1270.59
841_P844_H1.1270.59
61_I241_L1.1260.59
840_R848_T1.1260.59
70_P75_Q1.1250.59
551_T590_N1.1240.59
836_L839_A1.1240.59
367_V765_C1.1230.58
506_I535_I1.1230.58
579_D582_S1.1220.58
152_E322_L1.1220.58
859_K874_N1.1220.58
499_L797_I1.1210.58
858_A865_A1.1200.58
664_Y706_H1.1190.58
59_L62_K1.1190.58
773_V813_G1.1170.58
604_S638_V1.1150.58
838_E855_T1.1150.58
773_V793_R1.1150.58
121_I222_V1.1140.57
3_L7_L1.1130.57
38_V45_A1.1130.57
187_S613_R1.1130.57
671_V700_L1.1110.57
726_G776_L1.1090.57
187_S260_L1.1080.57
844_H857_K1.1070.57
866_T871_K1.1070.57
867_K873_K1.1070.57
851_R862_A1.1060.57
5_T16_L1.1040.56
86_N93_N1.1030.56
133_L153_A1.1030.56
558_L562_L1.1030.56
860_T863_T1.1020.56
833_V836_L1.1010.56
860_T867_K1.1010.56
110_S122_P1.1000.56
848_T851_R1.1000.56
20_A24_F1.0980.56
605_D608_M1.0970.56
856_N863_T1.0960.56
9_K17_E1.0950.56
672_G719_D1.0950.56
690_D730_K1.0940.55
836_L846_L1.0940.55
849_E852_R1.0930.55
859_K871_K1.0930.55
388_M430_W1.0910.55
838_E872_L1.0910.55
106_P148_E1.0910.55
113_V361_D1.0900.55
814_V818_A1.0890.55
376_K415_E1.0880.55
867_K870_R1.0870.55
498_C869_L1.0870.55
456_L473_A1.0860.55
799_S803_H1.0860.55
861_L872_L1.0860.55
42_R45_A1.0860.55
849_E857_K1.0860.55
97_Q772_I1.0850.54
798_L817_C1.0840.54
4_I11_V1.0840.54
761_L765_C1.0830.54
74_A495_A1.0830.54
735_V769_Y1.0830.54
243_K724_I1.0820.54
846_L852_R1.0820.54
493_E859_K1.0820.54
852_R856_N1.0810.54
832_D836_L1.0810.54
595_V604_S1.0790.54
860_T868_E1.0790.54
75_Q597_H1.0780.54
866_T869_L1.0780.54
71_D874_N1.0750.53
865_A870_R1.0720.53
80_W88_R1.0720.53
433_G472_W1.0710.53
8_E23_K1.0710.53
8_E27_R1.0710.53
222_V733_E1.0710.53
40_L57_A1.0700.53
850_G854_K1.0700.53
844_H860_T1.0690.53
755_V803_H1.0670.53
835_K863_T1.0670.53
245_M252_M1.0670.53
12_S15_R1.0660.53
361_D398_E1.0660.53
863_T870_R1.0660.53
355_L697_L1.0660.53
493_E843_I1.0660.53
845_E859_K1.0660.53
241_L624_V1.0650.52
129_L133_L1.0640.52
219_M628_A1.0640.52
819_A835_K1.0640.52
804_I814_V1.0630.52
5_T15_R1.0630.52
265_I685_I1.0620.52
867_K872_L1.0620.52
822_I851_R1.0610.52
510_L593_R1.0600.52
4_I457_I1.0590.52
241_L739_L1.0590.52
741_Q745_A1.0580.52
834_L837_V1.0580.52
242_V322_L1.0570.52
849_E856_N1.0570.52
832_D866_T1.0570.52
126_W130_I1.0560.51
434_R650_F1.0560.51
833_V845_E1.0550.51
7_L10_T1.0540.51
70_P210_D1.0530.51
850_G853_S1.0530.51
46_N50_S1.0530.51
514_T528_A1.0530.51
730_K801_I1.0510.51
803_H814_V1.0500.51
836_L845_E1.0500.51
861_L868_E1.0500.51
465_R687_P1.0490.51
811_T860_T1.0490.51
173_I390_F1.0490.51
25_L30_V1.0480.51
53_A322_L1.0480.51
849_E863_T1.0480.51
792_P795_E1.0480.51
813_G828_A1.0480.51
85_A125_Q1.0470.51
467_A620_G1.0470.51
770_T820_G1.0460.50
836_L848_T1.0460.50
496_T580_L1.0450.50
136_N147_K1.0440.50
524_L536_V1.0430.50
669_A718_G1.0430.50
4_I14_D1.0410.50
545_P549_K1.0400.50
863_T868_E1.0400.50
21_A54_R1.0390.50
782_N794_V1.0390.50
4_I7_L1.0380.50
342_W384_D1.0380.50
836_L849_E1.0380.50
848_T853_S1.0380.50
16_L23_K1.0380.50
50_S547_V1.0370.49
838_E853_S1.0370.49
384_D430_W1.0370.49
773_V821_L1.0370.49
833_V855_T1.0370.49
242_V278_Q1.0360.49
837_V861_L1.0360.49
843_I855_T1.0360.49
6_I17_E1.0350.49
221_V261_F1.0350.49
6_I24_F1.0350.49
844_H870_R1.0350.49
87_A95_V1.0340.49
870_R874_N1.0340.49
840_R873_K1.0330.49
558_L801_I1.0330.49
836_L843_I1.0330.49
375_I501_S1.0320.49
453_L779_D1.0310.49
592_L611_L1.0310.49
641_G684_N1.0310.49
841_P860_T1.0310.49
74_A708_S1.0300.49
157_I858_A1.0300.49
841_P849_E1.0300.49
20_A26_E1.0300.49
88_R120_E1.0300.49
36_F40_L1.0290.49
498_C873_K1.0280.49
65_L539_S1.0280.49
494_P498_C1.0280.49
20_A29_A1.0270.48
74_A834_L1.0270.48
161_I560_Q1.0270.48
615_F618_T1.0260.48
15_R19_E1.0260.48
370_F374_H1.0260.48
852_R874_N1.0260.48
860_T866_T1.0260.48
91_V133_L1.0250.48
803_H822_I1.0250.48
5_T335_E1.0250.48
494_P871_K1.0250.48
832_D867_K1.0250.48
835_K851_R1.0250.48
838_E857_K1.0250.48
85_A506_I1.0240.48
838_E860_T1.0240.48
339_D502_S1.0230.48
13_P16_L1.0230.48
605_D646_Y1.0230.48
122_P126_W1.0220.48
363_I393_I1.0220.48
841_P855_T1.0210.48
844_H847_L1.0210.48
834_L857_K1.0200.48
22_Q62_K1.0190.48
391_G394_L1.0190.48
32_N35_T1.0190.48
793_R797_I1.0190.48
848_T852_R1.0190.48
11_V22_Q1.0180.47
105_R477_L1.0180.47
20_A23_K1.0180.47
283_W287_C1.0180.47
40_L54_R1.0170.47
364_V453_L1.0170.47
784_H807_D1.0160.47
351_C643_F1.0160.47
12_S16_L1.0150.47
767_E774_Q1.0150.47
51_Q474_F1.0140.47
56_A65_L1.0140.47
598_Q838_E1.0140.47
772_I815_V1.0140.47
23_K30_V1.0130.47
69_D716_V1.0130.47
339_D681_L1.0120.47
276_A279_G1.0120.47
746_Q809_D1.0110.47
831_K872_L1.0110.47
157_I161_I1.0100.47
8_E12_S1.0100.47
113_V732_L1.0090.47
85_A89_R1.0090.47
494_P497_Y1.0090.47
848_T857_K1.0090.47
387_V431_T1.0080.46
848_T856_N1.0080.46
779_D841_P1.0070.46
859_K863_T1.0070.46
856_N868_E1.0070.46
5_T8_E1.0060.46
46_N49_N1.0060.46
92_K215_R1.0050.46
260_L413_L1.0050.46
495_A852_R1.0050.46
861_L873_K1.0050.46
19_E27_R1.0040.46
837_V860_T1.0040.46
238_L279_G1.0040.46
834_L845_E1.0040.46
237_A714_L1.0030.46
836_L853_S1.0030.46
68_K83_I1.0030.46
150_T176_A1.0030.46
283_W286_V1.0030.46
778_G856_N1.0030.46
189_N506_I1.0020.46
287_C432_V1.0010.46
43_V46_N1.0010.46
203_E243_K1.0000.46
300_A304_G1.0000.46
334_D381_R1.0000.46
502_S769_Y1.0000.46
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
1qgrA10.99321000.279Contact Map
2bptA10.96691000.357Contact Map
3ibvA20.84471000.477Contact Map
3gjxA20.93381000.5Contact Map
2x19B10.89731000.503Contact Map
4fddA10.88011000.515Contact Map
3zkvA10.81961000.515Contact Map
4c0oA20.89041000.518Contact Map
4hatC10.89841000.523Contact Map
4fgvA10.9111000.53Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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