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test -force run

ID: 1505149248 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 1000 (999)
Sequences: 756 (655.5)
Seq/Len: 0.757
Nf(neff/√len): 20.7

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 0.757).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
164_P181_V3.5441.00
102_V111_Q2.8311.00
60_V91_G2.6850.99
350_V413_I2.6480.99
351_M367_Y2.5910.99
406_R414_E2.5880.99
204_D283_R2.5140.99
60_V87_A2.4950.99
72_G375_L2.4430.98
959_L968_M2.3560.98
366_L405_G2.3090.97
216_L258_N2.2930.97
260_V288_S2.2770.97
29_C54_A2.2550.97
216_L321_W2.2520.97
959_L962_A2.2220.96
258_N321_W2.2130.96
205_R230_T2.1800.96
117_N169_H2.1360.95
186_G319_A2.1260.95
72_G163_Q2.1160.95
210_A216_L2.1130.95
935_I964_V2.1070.95
216_L259_S2.1020.95
183_N375_L2.0740.94
258_N261_P2.0660.94
48_Y87_A2.0040.93
347_R369_G2.0010.93
31_N35_H1.9930.92
262_A290_D1.9760.92
91_G95_A1.9600.92
31_N34_N1.9440.91
957_D962_A1.9190.90
355_L367_Y1.9190.90
210_A259_S1.9140.90
209_I321_W1.9110.90
932_I968_M1.9060.90
204_D255_T1.9030.90
323_I348_F1.8970.90
258_N312_L1.8940.90
190_T216_L1.8810.89
369_G398_L1.8720.89
312_L321_W1.8680.89
210_A312_L1.8600.88
288_S319_A1.8360.88
223_G321_W1.8260.87
350_V405_G1.8240.87
258_N288_S1.8110.87
195_N219_Y1.7810.85
288_S321_W1.7680.85
450_A464_A1.7620.84
51_R55_N1.7530.84
216_L222_F1.7530.84
58_A94_L1.7510.84
223_G258_N1.7500.84
308_E326_P1.7400.83
194_I210_A1.7380.83
167_I378_L1.7360.83
190_T321_W1.7260.83
259_S321_W1.7170.82
74_F100_L1.7140.82
938_A967_N1.7040.82
260_V312_L1.6970.81
957_D961_V1.6910.81
436_E450_A1.6860.81
216_L312_L1.6810.80
190_T258_N1.6690.80
264_M321_W1.6690.80
284_L310_I1.6260.77
54_A94_L1.6210.77
442_H873_Y1.6140.77
288_S312_L1.6110.76
209_I259_S1.6080.76
261_P296_L1.6040.76
164_P375_L1.5970.76
258_N287_L1.5900.75
961_V968_M1.5890.75
190_T263_Q1.5810.75
959_L991_P1.5770.74
957_D983_L1.5760.74
210_A224_I1.5760.74
191_I216_L1.5690.74
423_V432_L1.5660.74
207_I234_P1.5640.73
69_F97_G1.5640.73
190_T320_I1.5490.72
352_N356_Q1.5440.72
258_N263_Q1.5400.72
957_D968_M1.5390.72
927_D930_A1.5330.71
315_A321_W1.5320.71
74_F167_I1.5310.71
190_T219_Y1.5280.71
210_A321_W1.5270.71
263_Q321_W1.5260.71
54_A57_A1.5240.71
216_L315_A1.5230.71
219_Y321_W1.5210.70
947_R993_V1.5210.70
76_A169_H1.5180.70
189_N345_N1.5160.70
936_W993_V1.5140.70
216_L287_L1.5080.69
211_S258_N1.5070.69
219_Y258_N1.5050.69
216_L288_S1.5050.69
15_T345_N1.5030.69
259_S315_A1.5010.69
391_H396_E1.5000.69
935_I968_M1.4990.69
959_L963_Q1.4970.69
194_I216_L1.4910.68
209_I263_Q1.4900.68
964_V987_M1.4890.68
259_S263_Q1.4850.68
190_T194_I1.4750.67
218_V321_W1.4730.67
315_A346_Q1.4690.67
286_L312_L1.4670.66
223_G259_S1.4660.66
216_L260_V1.4650.66
367_Y398_L1.4550.65
259_S312_L1.4520.65
236_P239_K1.4500.65
84_W126_G1.4500.65
259_S322_S1.4430.64
208_G259_S1.4430.64
194_I321_W1.4400.64
194_I288_S1.4370.64
932_I964_V1.4360.64
216_L322_S1.4350.64
210_A258_N1.4340.64
968_M982_R1.4330.64
208_G321_W1.4320.64
450_A462_I1.4200.63
882_M900_K1.4200.63
222_F264_M1.4190.63
257_W321_W1.4170.62
441_N877_H1.4170.62
211_S321_W1.4160.62
287_L312_L1.4090.62
86_I221_I1.4090.62
209_I260_V1.4090.62
76_A120_T1.4070.62
222_F260_V1.4070.62
224_I231_L1.4050.61
190_T288_S1.4040.61
209_I322_S1.4040.61
922_E947_R1.4020.61
210_A263_Q1.4020.61
959_L972_L1.4010.61
193_D321_W1.4000.61
208_G229_G1.3990.61
965_I987_M1.3980.61
190_T319_A1.3950.61
288_S318_A1.3920.60
191_I210_A1.3920.60
189_N368_I1.3910.60
264_M287_L1.3900.60
194_I258_N1.3880.60
29_C57_A1.3860.60
380_D383_T1.3860.60
216_L263_Q1.3850.60
351_M398_L1.3850.60
24_L187_A1.3840.60
209_I286_L1.3770.59
218_V222_F1.3760.59
383_T387_R1.3730.59
223_G312_L1.3720.59
798_M801_K1.3690.58
73_D97_G1.3650.58
22_G30_S1.3600.58
219_Y263_Q1.3550.57
92_T885_G1.3540.57
216_L219_Y1.3500.57
365_D400_Y1.3480.57
311_S323_I1.3470.57
210_A223_G1.3460.57
957_D960_V1.3450.56
223_G229_G1.3440.56
193_D197_R1.3410.56
462_I465_F1.3410.56
258_N322_S1.3400.56
194_I312_L1.3360.56
190_T259_S1.3360.56
190_T209_I1.3340.56
355_L373_L1.3340.56
200_V204_D1.3330.55
77_V89_V1.3320.55
313_G339_Y1.3320.55
209_I258_N1.3310.55
29_C94_L1.3300.55
210_A292_I1.3300.55
257_W260_V1.3290.55
190_T322_S1.3290.55
223_G315_A1.3290.55
218_V320_I1.3290.55
318_A321_W1.3280.55
99_Y217_S1.3270.55
261_P321_W1.3260.55
961_V984_M1.3210.54
296_L321_W1.3180.54
258_N320_I1.3180.54
223_G320_I1.3150.54
28_F32_V1.3140.54
757_N760_H1.3120.54
204_D229_G1.3100.53
79_L124_L1.3090.53
232_V248_L1.3040.53
54_A58_A1.3030.53
341_T350_V1.3020.53
454_G458_Q1.3020.53
369_G373_L1.3020.53
191_I312_L1.2930.52
208_G231_L1.2930.52
260_V321_W1.2920.52
191_I194_I1.2920.52
290_D415_F1.2900.52
957_D984_M1.2870.51
210_A287_L1.2850.51
194_I219_Y1.2830.51
194_I223_G1.2830.51
85_Q212_L1.2770.51
200_V229_G1.2730.50
208_G322_S1.2720.50
962_A968_M1.2710.50
182_M216_L1.2660.50
224_I258_N1.2660.50
194_I259_S1.2650.50
198_F310_I1.2650.50
113_T169_H1.2630.49
315_A339_Y1.2630.49
222_F321_W1.2620.49
204_D256_V1.2610.49
261_P264_M1.2610.49
259_S287_L1.2600.49
210_A290_D1.2600.49
439_L448_A1.2570.49
933_A993_V1.2560.49
929_E997_A1.2530.49
210_A346_Q1.2520.48
993_V996_I1.2510.48
956_G961_V1.2480.48
209_I320_I1.2480.48
190_T223_G1.2450.48
193_D292_I1.2450.48
432_L450_A1.2430.48
312_L315_A1.2380.47
959_L983_L1.2380.47
952_Y988_L1.2370.47
205_R254_V1.2360.47
190_T224_I1.2350.47
925_Q994_A1.2340.47
35_H38_A1.2340.47
209_I288_S1.2330.47
259_S264_M1.2320.47
220_D318_A1.2290.46
189_N320_I1.2280.46
208_G216_L1.2250.46
359_P362_T1.2220.46
884_P900_K1.2190.46
315_A337_V1.2160.45
208_G258_N1.2150.45
311_S339_Y1.2140.45
216_L223_G1.2140.45
952_Y956_G1.2140.45
391_H398_L1.2120.45
258_N264_M1.2080.45
77_V88_S1.2080.45
223_G310_I1.2080.45
193_D260_V1.2070.45
190_T222_F1.2060.45
193_D224_I1.2050.44
257_W315_A1.2050.44
219_Y320_I1.2030.44
936_W956_G1.2020.44
291_W321_W1.2010.44
194_I209_I1.2000.44
312_L320_I1.2000.44
220_D319_A1.1980.44
260_V264_M1.1970.44
925_Q947_R1.1970.44
186_G373_L1.1960.44
220_D415_F1.1950.44
210_A315_A1.1940.44
193_D209_I1.1930.43
218_V258_N1.1930.43
950_N953_I1.1910.43
962_A991_P1.1890.43
219_Y259_S1.1880.43
223_G287_L1.1880.43
189_N321_W1.1880.43
287_L321_W1.1870.43
25_H57_A1.1860.43
455_N458_Q1.1840.43
260_V319_A1.1830.43
222_F312_L1.1820.42
209_I216_L1.1820.42
211_S287_L1.1820.42
238_Q244_Y1.1790.42
262_A291_W1.1790.42
287_L292_I1.1790.42
318_A368_I1.1750.42
187_A222_F1.1750.42
683_E689_G1.1750.42
440_Q447_S1.1740.42
312_L318_A1.1730.42
56_F84_W1.1710.42
206_I321_W1.1710.42
440_Q448_A1.1700.41
325_Y338_P1.1700.41
207_I256_V1.1670.41
13_N345_N1.1650.41
247_E251_K1.1600.41
962_A984_M1.1570.40
968_M990_T1.1570.40
2_P5_V1.1560.40
959_L1000_L1.1530.40
193_D198_F1.1520.40
117_N380_D1.1490.40
292_I297_P1.1480.40
98_V164_P1.1470.40
186_G189_N1.1470.40
210_A219_Y1.1460.39
193_D344_L1.1440.39
367_Y392_P1.1410.39
168_I320_I1.1380.39
190_T220_D1.1370.39
182_M319_A1.1360.39
341_T412_V1.1360.39
381_A385_N1.1350.39
208_G300_L1.1340.39
218_V260_V1.1340.39
389_I398_L1.1340.39
968_M1000_L1.1330.38
408_R412_V1.1330.38
466_I870_I1.1330.38
922_E948_D1.1300.38
106_Q114_I1.1300.38
355_L392_P1.1300.38
438_V874_L1.1290.38
349_Y357_L1.1290.38
968_M984_M1.1290.38
249_I267_L1.1280.38
710_D780_G1.1260.38
210_A260_V1.1240.38
29_C58_A1.1220.38
240_N312_L1.1200.37
355_L398_L1.1200.37
287_L315_A1.1180.37
223_G290_D1.1160.37
706_Y710_D1.1160.37
263_Q320_I1.1130.37
288_S313_G1.1120.37
79_L85_Q1.1110.37
216_L318_A1.1110.37
367_Y373_L1.1110.37
261_P320_I1.1100.37
239_K287_L1.1080.36
210_A239_K1.1070.36
258_N267_L1.1060.36
311_S315_A1.1060.36
209_I219_Y1.1050.36
190_T210_A1.1030.36
288_S315_A1.1000.36
187_A223_G1.0980.36
878_L882_M1.0970.36
204_D288_S1.0960.35
23_L26_D1.0950.35
223_G256_V1.0930.35
321_W346_Q1.0930.35
43_Y83_I1.0930.35
363_A416_L1.0920.35
194_I290_D1.0910.35
57_A94_L1.0910.35
312_L324_Y1.0900.35
900_K906_L1.0900.35
959_L984_M1.0900.35
39_A49_S1.0900.35
435_I870_I1.0890.35
258_N315_A1.0850.35
71_K163_Q1.0850.35
73_D121_R1.0830.34
286_L321_W1.0830.34
207_I213_A1.0820.34
222_F288_S1.0820.34
234_P245_W1.0820.34
198_F258_N1.0810.34
258_N284_L1.0800.34
69_F75_A1.0790.34
114_I213_A1.0790.34
204_D241_N1.0790.34
948_D994_A1.0780.34
232_V254_V1.0770.34
216_L257_W1.0750.34
425_I452_V1.0750.34
28_F50_Y1.0740.34
373_L396_E1.0740.34
981_E984_M1.0740.34
209_I234_P1.0730.34
259_S267_L1.0730.34
181_V209_I1.0720.34
287_L297_P1.0690.33
91_G161_P1.0680.33
449_V452_V1.0680.33
223_G264_M1.0660.33
126_G129_G1.0660.33
392_P398_L1.0660.33
204_D284_L1.0660.33
894_P906_L1.0650.33
932_I935_I1.0650.33
193_D312_L1.0640.33
190_T318_A1.0630.33
239_K258_N1.0630.33
42_F252_E1.0610.33
884_P902_D1.0610.33
923_A946_G1.0610.33
186_G257_W1.0610.33
968_M983_L1.0610.33
57_A91_G1.0600.33
39_A47_Y1.0600.33
951_F956_G1.0600.33
209_I236_P1.0590.33
348_F366_L1.0590.33
323_I339_Y1.0580.33
259_S310_I1.0570.32
965_I969_R1.0570.32
222_F258_N1.0570.32
58_A62_K1.0560.32
921_E944_T1.0540.32
224_I292_I1.0540.32
465_F906_L1.0540.32
219_Y292_I1.0530.32
223_G257_W1.0520.32
389_I451_M1.0510.32
407_Y425_I1.0500.32
292_I322_S1.0490.32
961_V989_Q1.0490.32
293_P321_W1.0470.32
209_I290_D1.0470.32
776_A780_G1.0460.32
262_A292_I1.0460.32
297_P315_A1.0430.31
348_F405_G1.0430.31
218_V261_P1.0420.31
261_P312_L1.0420.31
200_V219_Y1.0410.31
257_W262_A1.0410.31
292_I311_S1.0410.31
229_G321_W1.0400.31
264_M312_L1.0390.31
209_I241_N1.0390.31
69_F73_D1.0390.31
683_E710_D1.0380.31
284_L322_S1.0380.31
258_N262_A1.0370.31
63_A67_K1.0370.31
555_L558_W1.0370.31
206_I216_L1.0360.31
207_I293_P1.0350.31
191_I222_F1.0350.31
926_D947_R1.0330.31
351_M355_L1.0320.31
195_N223_G1.0320.31
99_Y218_V1.0300.30
189_N241_N1.0300.30
193_D258_N1.0290.30
229_G254_V1.0290.30
219_Y223_G1.0290.30
207_I255_T1.0280.30
187_A320_I1.0280.30
367_Y419_E1.0270.30
223_G260_V1.0250.30
258_N296_L1.0250.30
197_R296_L1.0240.30
204_D258_N1.0240.30
255_T384_A1.0230.30
190_T260_V1.0220.30
80_E126_G1.0210.30
219_Y310_I1.0210.30
258_N292_I1.0210.30
256_V288_S1.0210.30
204_D223_G1.0200.30
193_D310_I1.0190.30
258_N285_A1.0190.30
187_A226_T1.0180.30
196_E344_L1.0180.30
933_A947_R1.0180.30
191_I310_I1.0170.30
239_K260_V1.0170.30
683_E706_Y1.0170.30
265_Q293_P1.0160.29
259_S288_S1.0160.29
287_L322_S1.0150.29
198_F209_I1.0140.29
682_L685_G1.0130.29
878_L900_K1.0130.29
926_D930_A1.0130.29
297_P337_V1.0130.29
193_D212_L1.0120.29
231_L264_M1.0110.29
878_L884_P1.0110.29
210_A234_P1.0100.29
291_W300_L1.0100.29
258_N297_P1.0090.29
189_N229_G1.0080.29
291_W415_F1.0080.29
350_V367_Y1.0080.29
220_D339_Y1.0080.29
689_G751_D1.0070.29
467_E893_I1.0060.29
450_A465_F1.0040.29
81_K234_P1.0030.29
209_I287_L1.0020.28
435_I439_L1.0020.28
968_M972_L1.0020.28
218_V288_S1.0020.28
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
2vsqA10.5921000.404Contact Map
4dg8A10.4911000.44Contact Map
4iz6A20.5771000.481Contact Map
4r0mB20.5861000.492Contact Map
4d4iA101000.543Contact Map
1amuA20.5031000.551Contact Map
3tsyA10.4751000.575Contact Map
4gr5A40.4071000.594Contact Map
3g7sA20.471000.596Contact Map
3vnrA10.4891000.597Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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