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ID: 1506954968 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 102 (101)
Sequences: 726 (450.1)
Seq/Len: 7.188
Nf(neff/√len): 44.8

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 7.188).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
43_D55_R3.7031.00
46_R83_V3.1441.00
52_V83_V3.0071.00
32_F89_Q2.9631.00
42_T94_M2.1181.00
46_R52_V2.0961.00
92_A96_E2.0431.00
84_P87_K1.9101.00
88_E92_A1.8341.00
77_E80_L1.7721.00
13_E17_K1.6981.00
49_S68_D1.6341.00
86_L90_I1.6111.00
87_K91_Q1.5781.00
31_E89_Q1.5120.99
9_E13_E1.4900.99
53_M57_I1.4540.99
47_L68_D1.3710.99
4_F8_A1.3230.98
96_E100_S1.2920.98
12_E16_A1.2650.97
12_E15_V1.2490.97
76_T79_M1.2460.97
32_F86_L1.2300.97
92_A95_R1.2220.96
41_M54_D1.2160.96
38_D93_W1.1740.95
25_Y94_M1.1630.95
1_I71_N1.1430.94
85_E89_Q1.1300.94
5_K9_E1.1030.93
8_A11_V1.0920.92
61_H72_R1.0900.92
60_R73_Q1.0860.92
6_L16_A1.0830.92
50_G73_Q1.0770.92
3_K13_E1.0740.92
75_L79_M1.0680.91
59_L77_E1.0660.91
46_R81_E1.0520.90
57_I80_L1.0390.90
6_L13_E1.0330.89
33_R38_D1.0250.89
36_L39_T1.0170.88
32_F90_I1.0160.88
47_L70_F1.0020.87
13_E16_A1.0020.87
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
3m62A10.990299.90.222Contact Map
4wz3B10.852999.60.498Contact Map
2kreA10.980499.50.52Contact Map
2c2lA40.990299.50.522Contact Map
2f42A10.970699.50.538Contact Map
2kr4A10.833399.40.555Contact Map
1wgmA10.911899.40.568Contact Map
4wz0A10.941299.30.571Contact Map
3htkC1199.30.579Contact Map
2z6gA1099.20.597Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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