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ID: 1513369998 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 363 (349)
Sequences: 7156 (4349)
Seq/Len: 20.504
Nf(neff/√len): 232.8

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 20.504).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
119_L263_A3.4801.00
157_I193_A3.1301.00
122_L262_R3.0561.00
288_T313_Y3.0461.00
159_I189_A2.9851.00
94_G155_H2.9721.00
273_I332_A2.9381.00
117_N147_Y2.9281.00
233_S236_Q2.7081.00
128_L227_S2.6821.00
130_T229_L2.6311.00
272_R279_V2.6291.00
150_R170_K2.5161.00
324_E328_K2.4761.00
52_L95_A2.4381.00
90_H163_I2.4211.00
34_D271_K2.2191.00
294_L306_I2.2021.00
293_N297_E2.1931.00
120_A144_M2.1591.00
265_I284_I2.1411.00
108_E117_N2.1181.00
78_G101_P2.1041.00
321_A325_Y2.0811.00
106_T109_E2.0551.00
20_H197_D2.0271.00
85_N196_E2.0071.00
287_A292_R1.9941.00
82_F97_F1.9821.00
154_D200_I1.9571.00
279_V330_I1.9551.00
94_G175_N1.9311.00
206_M215_A1.9151.00
118_K286_I1.8891.00
11_M16_A1.8641.00
233_S304_P1.8631.00
141_L202_L1.8351.00
116_L144_M1.8321.00
206_M227_S1.8201.00
7_F19_L1.8121.00
287_A295_I1.8051.00
322_I336_A1.7761.00
87_F177_R1.7751.00
319_D340_A1.7691.00
129_V140_T1.7671.00
64_F93_V1.7521.00
100_I165_F1.7411.00
9_K13_E1.7371.00
291_V303_I1.7271.00
206_M212_I1.7091.00
294_L299_K1.6991.00
34_D153_K1.6891.00
16_A31_V1.6881.00
316_V324_E1.6871.00
238_V261_L1.6681.00
86_F95_A1.6621.00
317_S320_D1.6331.00
285_M315_M1.6281.00
121_L147_Y1.6211.00
272_R330_I1.6161.00
141_L226_M1.6141.00
271_K335_E1.6051.00
321_A324_E1.5941.00
281_A325_Y1.5841.00
146_D150_R1.5751.00
154_D172_C1.5721.00
323_M327_Q1.5661.00
201_I218_A1.5611.00
212_I244_I1.5491.00
82_F99_L1.5461.00
127_V224_L1.5431.00
209_L213_Q1.5231.00
30_R197_D1.5161.00
335_E338_N1.5091.00
25_N48_L1.4961.00
103_Q109_E1.4961.00
11_M19_L1.4881.00
84_V97_F1.4821.00
213_Q217_E1.4821.00
10_L14_T1.4791.00
299_K302_Q1.4501.00
4_I51_M1.4431.00
20_H30_R1.4331.00
185_S188_A1.4261.00
89_Q175_N1.4241.00
68_G88_Q1.4171.00
37_R40_Y1.4151.00
26_P37_R1.4081.00
146_D170_K1.3980.99
290_A313_Y1.3920.99
306_I310_G1.3800.99
190_L214_L1.3800.99
326_L336_A1.3770.99
130_T231_T1.3710.99
149_N156_I1.3700.99
127_V263_A1.3680.99
63_E67_S1.3360.99
331_I335_E1.3330.99
150_R153_K1.3250.99
191_R208_D1.3230.99
126_L225_V1.3220.99
107_V143_A1.3090.99
62_K66_E1.3080.99
53_Y61_I1.2990.99
279_V325_Y1.2940.99
148_A226_M1.2910.99
119_L127_V1.2870.99
57_P60_K1.2810.99
133_T198_P1.2760.99
288_T306_I1.2710.99
147_Y151_M1.2640.99
302_Q305_S1.2610.99
331_I336_A1.2590.99
193_A201_I1.2580.99
159_I179_V1.2570.99
32_H272_R1.2560.99
177_R193_A1.2430.98
29_L38_V1.2430.98
148_A200_I1.2430.98
124_K262_R1.2420.98
94_G157_I1.2380.98
235_S285_M1.2370.98
69_D183_T1.2360.98
159_I193_A1.2340.98
212_I241_I1.2300.98
64_F183_T1.2250.98
14_T36_Q1.2210.98
326_L333_P1.2110.98
327_Q330_I1.2090.98
112_L282_L1.1980.98
49_K53_Y1.1930.98
268_T318_L1.1920.98
59_E182_H1.1880.98
308_Q320_D1.1800.98
282_L316_V1.1770.98
187_A210_E1.1690.97
12_V80_A1.1560.97
85_N96_V1.1510.97
189_A193_A1.1440.97
107_V117_N1.1390.97
153_K171_N1.1390.97
19_L29_L1.1350.97
208_D211_T1.1300.97
105_R109_E1.1290.97
111_G280_V1.1260.97
325_Y330_I1.1220.97
217_E243_E1.1120.96
59_E276_P1.1070.96
33_G278_R1.1060.96
108_E147_Y1.1060.96
59_E183_T1.1010.96
37_R152_R1.0990.96
262_R292_R1.0980.96
61_I183_T1.0960.96
15_G32_H1.0890.96
155_H175_N1.0840.96
64_F182_H1.0810.95
28_I37_R1.0760.95
264_V285_M1.0670.95
270_F335_E1.0660.95
257_L261_L1.0650.95
130_T237_T1.0640.95
11_M29_L1.0560.95
285_M291_V1.0540.95
16_A29_L1.0520.95
8_L82_F1.0480.94
316_V321_A1.0410.94
258_A295_I1.0350.94
133_T223_H1.0340.94
22_S28_I1.0260.94
237_T264_V1.0260.94
179_V183_T1.0250.93
270_F331_I1.0180.93
261_L264_V1.0180.93
306_I313_Y1.0150.93
140_T267_Q1.0090.93
31_V36_Q1.0070.93
346_F350_V1.0050.93
245_F276_P1.0010.92
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
2ewvA10.94211000.27Contact Map
3jvvA30.92841000.298Contact Map
4ksrA301000.336Contact Map
4phtA30.8761000.352Contact Map
1p9rA10.87331000.383Contact Map
2eyuA20.67771000.48Contact Map
2oap120.94211000.529Contact Map
4ihqA30.94771000.558Contact Map
2gzaA30.78241000.61Contact Map
4bloA120.674999.90.727Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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