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slc3a2

ID: 1514469152 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 630 (423)
Sequences: 32458 (21722.9)
Seq/Len: 76.733
Nf(neff/√len): 1056.2

Jackhmmer Results: (2016_04)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 76.733).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
242_A292_S3.5571.00
291_D340_A3.5301.00
249_D300_K3.4611.00
233_F247_R3.3941.00
298_K343_D3.3241.00
296_S299_K3.3041.00
296_S300_K3.0911.00
455_L621_G3.0861.00
286_S289_D3.0121.00
266_K279_Q2.8551.00
245_K292_S2.8551.00
245_K296_S2.8281.00
242_A289_D2.7991.00
252_S300_K2.7271.00
242_A245_K2.5901.00
454_Q523_F2.5151.00
327_A362_E2.4891.00
248_L300_K2.3851.00
251_L256_V2.3711.00
253_S524_R2.2521.00
297_A304_V2.2501.00
297_A302_I2.2481.00
364_Q368_K2.1581.00
335_E366_I2.1461.00
554_I562_R2.1221.00
459_T623_L2.0851.00
433_W470_D2.0551.00
459_T553_Y2.0231.00
458_F527_S2.0161.00
528_D532_K2.0061.00
451_R522_L1.9971.00
244_L293_L1.9451.00
233_F246_G1.9221.00
617_E620_E1.9181.00
287_K340_A1.9131.00
521_S524_R1.9051.00
455_L522_L1.8871.00
266_K275_T1.8831.00
292_S296_S1.8601.00
259_L297_A1.8321.00
575_S613_R1.7951.00
223_A527_S1.7901.00
222_G257_K1.7801.00
575_S615_K1.7781.00
556_H562_R1.7661.00
266_K282_P1.7521.00
278_L332_D1.7481.00
567_L621_G1.7291.00
295_Q299_K1.7271.00
290_F306_L1.7241.00
294_L304_V1.7241.00
320_S323_V1.7111.00
552_S566_V1.7101.00
227_I244_L1.7091.00
542_H554_I1.6901.00
335_E370_F1.6801.00
248_L297_A1.6601.00
525_R604_E1.6551.00
288_E291_D1.6541.00
235_G238_A1.6421.00
257_K531_S1.6121.00
334_L363_W1.5981.00
429_R433_W1.5901.00
533_E561_E1.5841.00
271_D274_Q1.5821.00
573_G615_K1.5801.00
290_F294_L1.5761.00
549_G570_G1.5621.00
338_L374_R1.5611.00
278_L333_A1.5601.00
595_L598_S1.5511.00
331_K362_E1.5481.00
328_T332_D1.5381.00
224_L258_G1.5361.00
368_K397_D1.5351.00
439_R442_T1.5341.00
354_K359_F1.5301.00
440_L444_F1.5201.00
542_H556_H1.5191.00
473_G519_L1.5111.00
287_K336_F1.4841.00
447_A451_R1.4801.00
264_I290_F1.4751.00
430_W433_W1.4731.00
242_A246_G1.4671.00
290_F342_V1.4651.00
439_R443_S1.4491.00
525_R528_D1.4481.00
324_D328_T1.4461.00
253_S520_L1.4431.00
221_T537_L1.4391.00
554_I564_L1.4371.00
459_T565_V1.4171.00
319_F326_V1.4171.00
305_I344_G1.4161.00
452_L567_L1.4121.00
279_Q287_K1.4030.99
438_A442_T1.4020.99
278_L336_F1.3900.99
289_D292_S1.3850.99
318_W321_T1.3680.99
521_S525_R1.3570.99
308_L334_L1.3440.99
366_I369_G1.3400.99
276_D279_Q1.3390.99
336_F340_A1.3390.99
310_P333_A1.3380.99
313_R319_F1.3290.99
557_W560_N1.3270.99
261_L264_I1.3250.99
247_R250_Y1.3220.99
533_E563_F1.3210.99
244_L261_L1.3130.99
329_K332_D1.3100.99
524_R528_D1.3090.99
240_N243_G1.3080.99
325_T328_T1.3040.99
456_M567_L1.2970.99
520_L524_R1.2840.99
491_W494_S1.2820.99
577_G613_R1.2800.99
553_Y565_V1.2800.99
331_K366_I1.2800.99
318_W323_V1.2760.99
414_H417_S1.2750.99
226_R262_G1.2750.99
438_A441_L1.2720.99
321_T330_V1.2670.99
287_K291_D1.2670.99
355_D358_S1.2660.99
227_I232_A1.2620.99
327_A331_K1.2610.99
326_V329_K1.2590.99
390_S393_E1.2410.98
455_L526_L1.2320.98
339_Q370_F1.2320.98
439_R444_F1.2290.98
288_E292_S1.2250.98
439_R445_L1.2250.98
249_D253_S1.2140.98
535_S563_F1.2080.98
319_F323_V1.2070.98
252_S302_I1.2060.98
429_R432_S1.2050.98
293_L297_A1.2000.98
250_Y520_L1.1970.98
222_G258_G1.1880.98
325_T329_K1.1870.98
432_S453_Y1.1810.98
328_T366_I1.1780.98
540_D556_H1.1700.97
423_L426_T1.1630.97
318_W326_V1.1520.97
258_G303_R1.1510.97
538_H557_W1.1490.97
362_E366_I1.1450.97
486_A489_M1.1430.97
365_N369_G1.1420.97
241_L280_I1.1320.97
323_V326_V1.1280.97
573_G617_E1.1270.97
440_L443_S1.1260.97
595_L622_L1.1240.97
441_L445_L1.1210.97
306_L342_V1.1200.96
270_D273_A1.1180.96
449_L619_H1.1170.96
291_D295_Q1.1160.96
447_A516_P1.1110.96
319_F330_V1.1060.96
419_V422_Y1.1030.96
570_G574_L1.1020.96
223_A256_V1.1010.96
349_D352_N1.0950.96
415_T419_V1.0940.96
571_D619_H1.0920.96
410_S414_H1.0920.96
438_A443_S1.0870.96
245_K293_L1.0830.96
416_K420_T1.0800.95
280_I290_F1.0790.95
278_L329_K1.0770.95
221_T538_H1.0720.95
474_L490_L1.0720.95
251_L302_I1.0710.95
326_V331_K1.0700.95
451_R519_L1.0630.95
396_K400_L1.0610.95
595_L624_L1.0580.95
303_R343_D1.0560.95
535_S561_E1.0560.95
533_E625_R1.0480.94
335_E339_Q1.0480.94
441_L450_L1.0480.94
249_D296_S1.0460.94
261_L293_L1.0420.94
364_Q376_L1.0370.94
310_P330_V1.0350.94
338_L371_S1.0270.94
321_T326_V1.0260.94
207_A292_S1.0250.93
248_L296_S1.0240.93
292_S295_Q1.0230.93
229_D244_L1.0230.93
412_G416_K1.0230.93
595_L599_T1.0210.93
441_L444_F1.0200.93
466_F523_F1.0190.93
390_S394_S1.0150.93
258_G305_I1.0150.93
324_D327_A1.0130.93
251_L259_L1.0120.93
264_I306_L1.0110.93
411_T414_H1.0070.93
595_L600_Q1.0060.93
366_I370_F1.0030.93
398_L401_T1.0010.92
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
2dh2A10.66511000.405Contact Map
3wy2A201000.427Contact Map
1uokA10.63811000.428Contact Map
2zicA10.63021000.432Contact Map
2ze0A10.63811000.432Contact Map
4aieA10.62541000.432Contact Map
3zo9A20.66671000.432Contact Map
4mb1A10.63021000.433Contact Map
3aj7A10.6461000.434Contact Map
1zjaA20.63971000.434Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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