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Asn1 protein

ID: 1515011081 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 585 (557)
Sequences: 726 (284.4)
Seq/Len: 1.303
Nf(neff/√len): 12.1

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.303).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
279_K291_E3.6081.00
280_E444_H3.3671.00
118_D220_K3.3431.00
120_V142_V3.1511.00
360_N363_E2.9311.00
120_V221_R2.5631.00
91_H104_H2.4080.99
304_E504_R2.3630.99
214_F241_V2.3220.99
263_H290_H2.2480.99
118_D221_R2.2080.99
2_C75_N2.1970.99
477_M500_M2.1810.99
427_W431_K2.1420.98
27_R166_D2.0710.98
135_A157_I1.9850.97
524_S527_K1.9800.97
87_Q116_M1.9760.97
263_H288_M1.9330.97
524_S528_A1.9280.96
147_I187_F1.8730.96
431_K434_D1.8520.95
348_I404_I1.8190.95
35_W60_D1.7400.93
213_A406_E1.7370.93
219_V248_H1.7250.92
65_N69_S1.6700.91
469_A497_C1.6680.91
281_V445_I1.6580.90
95_T100_E1.6330.89
140_I404_I1.6280.89
142_V396_V1.6170.89
276_K280_E1.6160.89
478_M505_F1.6100.88
109_H116_M1.6050.88
139_A142_V1.5990.88
61_Q75_N1.5670.87
173_I514_T1.5640.86
50_R75_N1.5620.86
51_L62_P1.5540.86
239_S441_L1.5520.86
34_D62_P1.5480.86
53_I75_N1.5450.86
124_V135_A1.5400.85
34_D37_G1.5390.85
150_G154_S1.5320.85
320_A324_M1.5290.85
486_P489_T1.5230.84
297_Q301_D1.5220.84
279_K283_N1.5210.84
147_I172_E1.5180.84
310_T489_T1.5140.84
33_P37_G1.5090.84
55_D225_D1.5050.83
85_R93_F1.4960.83
191_Y405_N1.4920.83
126_L131_N1.4910.83
50_R53_I1.4740.82
526_A529_V1.4680.81
218_V244_V1.4570.81
62_P75_N1.4470.80
210_L407_A1.4410.80
516_P530_E1.4340.79
240_L429_L1.4280.79
61_Q77_E1.4230.78
545_A554_Y1.4220.78
143_T388_S1.4140.78
81_H85_R1.4040.77
51_L61_Q1.3890.76
362_E366_R1.3820.76
521_V554_Y1.3810.76
158_S163_G1.3790.75
34_D48_H1.3790.75
228_F338_M1.3770.75
524_S532_D1.3750.75
50_R61_Q1.3720.75
158_S164_L1.3720.75
534_Q537_G1.3650.74
37_G48_H1.3630.74
93_F101_V1.3630.74
2_C53_I1.3620.74
31_R50_R1.3470.73
33_P51_L1.3460.73
229_G336_V1.3370.72
33_P48_H1.3300.72
143_T384_N1.3270.71
104_H118_D1.3250.71
527_K532_D1.3200.71
350_G364_L1.3200.71
410_I428_V1.3170.71
231_L331_I1.3060.70
34_D51_L1.3010.69
261_K288_M1.3010.69
240_L446_L1.2930.68
387_T394_A1.2930.68
62_P77_E1.2910.68
178_H217_A1.2870.68
31_R34_D1.2860.68
525_T528_A1.2770.67
474_T477_M1.2760.67
147_I171_F1.2750.67
31_R51_L1.2750.67
2_C77_E1.2700.66
53_I62_P1.2680.66
21_V45_Y1.2640.66
32_G51_L1.2610.66
248_H251_G1.2580.65
51_L75_N1.2510.65
161_M518_G1.2500.65
53_I61_Q1.2480.65
165_N509_N1.2470.64
478_M519_P1.2430.64
118_D402_E1.2430.64
343_E355_F1.2360.63
250_A261_K1.2350.63
474_T478_M1.2320.63
2_C50_R1.2300.63
516_P522_A1.2260.62
532_D536_S1.2240.62
91_H108_E1.2210.62
2_C61_Q1.2190.62
330_K394_A1.2180.62
2_C31_R1.2140.61
55_D241_V1.2110.61
528_A532_D1.2110.61
64_Y68_K1.2100.61
252_T257_R1.2070.61
34_D61_Q1.2050.60
214_F325_F1.1970.60
2_C62_P1.1960.60
175_P178_H1.1940.59
371_K533_A1.1920.59
214_F244_V1.1910.59
93_F100_E1.1910.59
33_P59_G1.1880.59
81_H223_M1.1870.59
230_V264_S1.1870.59
164_L171_F1.1870.59
480_N483_F1.1850.59
231_L341_S1.1840.58
495_A518_G1.1840.58
260_T334_L1.1800.58
497_C501_I1.1790.58
106_Y113_F1.1780.58
538_N546_A1.1780.58
535_W539_L1.1770.58
163_G417_I1.1750.58
50_R62_P1.1690.57
32_G50_R1.1670.57
267_V291_E1.1670.57
245_T348_I1.1670.57
79_Y98_D1.1620.56
209_A213_A1.1610.56
61_Q98_D1.1600.56
32_G62_P1.1590.56
99_C338_M1.1560.56
475_D493_K1.1540.56
179_L188_K1.1520.55
525_T529_V1.1510.55
548_G553_A1.1480.55
388_S546_A1.1410.54
257_R260_T1.1400.54
75_N393_E1.1360.54
272_S276_K1.1360.54
357_K532_D1.1360.54
33_P62_P1.1340.54
296_V460_S1.1340.54
529_V536_S1.1330.53
74_V99_C1.1320.53
122_S143_T1.1310.53
536_S539_L1.1300.53
252_T255_A1.1300.53
516_P523_C1.1270.53
487_H513_L1.1220.52
65_N68_K1.1220.52
491_T539_L1.1200.52
544_R550_H1.1180.52
545_A549_V1.1180.52
330_K333_S1.1180.52
147_I155_V1.1170.52
239_S453_F1.1140.52
434_D446_L1.1140.52
411_D414_W1.1110.51
249_L252_T1.1110.51
114_I163_G1.1110.51
315_V495_A1.1100.51
178_H405_N1.1070.51
287_T368_T1.1070.51
65_N107_E1.1060.51
542_S550_H1.1050.51
30_H33_P1.1040.51
534_Q539_L1.1030.50
31_R62_P1.0980.50
301_D384_N1.0940.50
66_E91_H1.0930.49
216_K402_E1.0910.49
432_A446_L1.0900.49
152_D248_H1.0900.49
304_E505_F1.0890.49
296_V464_G1.0880.49
224_T404_I1.0880.49
289_H343_E1.0870.49
25_S47_S1.0860.49
31_R75_N1.0840.49
125_L134_I1.0840.49
384_N390_W1.0810.48
48_H226_V1.0810.48
146_Y385_K1.0810.48
479_S482_K1.0800.48
329_R384_N1.0770.48
337_K393_E1.0770.48
53_I77_E1.0760.48
33_P50_R1.0760.48
90_S108_E1.0730.47
29_K36_S1.0730.47
519_P525_T1.0720.47
18_R38_M1.0680.47
532_D535_W1.0680.47
153_G225_D1.0670.47
1_M32_G1.0650.47
34_D50_R1.0650.47
338_M384_N1.0620.46
317_T381_L1.0610.46
142_V467_A1.0600.46
113_F134_I1.0590.46
490_P499_R1.0590.46
187_F501_I1.0540.46
270_E293_T1.0530.46
452_Q456_G1.0520.45
62_P98_D1.0500.45
333_S384_N1.0430.45
241_V244_V1.0430.45
326_L389_A1.0420.44
30_H34_D1.0400.44
551_L554_Y1.0390.44
55_D77_E1.0380.44
316_T462_I1.0370.44
516_P520_S1.0360.44
171_F366_R1.0360.44
324_M355_F1.0340.44
84_L88_L1.0340.44
187_F241_V1.0330.44
2_C388_S1.0310.43
464_G468_H1.0310.43
254_A257_R1.0300.43
250_A256_K1.0260.43
219_V283_N1.0260.43
135_A145_L1.0230.43
274_D289_H1.0220.42
34_D77_E1.0210.42
2_C98_D1.0210.42
149_W155_V1.0200.42
31_R61_Q1.0190.42
252_T256_K1.0180.42
132_S345_S1.0180.42
305_D332_K1.0170.42
75_N98_D1.0160.42
305_D329_R1.0160.42
88_L108_E1.0160.42
139_A401_K1.0150.42
102_I241_V1.0120.42
348_I519_P1.0110.41
268_G311_E1.0070.41
228_F333_S1.0070.41
292_F330_K1.0070.41
485_Y489_T1.0040.41
81_H120_V1.0030.41
262_L314_D1.0030.41
525_T553_A1.0020.41
139_A404_I1.0020.41
2_C393_E1.0020.41
343_E383_A1.0010.40
527_K531_W1.0010.40
83_Q118_D1.0010.40
55_D62_P1.0000.40
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
1ct9A40.85471000.312Contact Map
1q15A40.77611000.519Contact Map
1jgtA20.77091000.539Contact Map
1ao0A40.63251000.782Contact Map
1xffA20.3351000.797Contact Map
1ecfA20.67011000.803Contact Map
4amvA30.81000.807Contact Map
1te5A20.29911000.808Contact Map
3mdnA40.28381000.832Contact Map
4zflA1201000.839Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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