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ASN1 protein

ID: 1515105381 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 572 (546)
Sequences: 946 (465.5)
Seq/Len: 1.733
Nf(neff/√len): 19.9

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.733).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
304_R316_E4.1791.00
305_K473_E3.4321.00
34_W59_A3.3501.00
90_Y103_P3.3091.00
141_I421_V3.2761.00
139_I429_L2.8091.00
119_M141_I2.7781.00
117_D222_K2.7521.00
64_S68_E2.7351.00
288_H313_I2.7131.00
20_A44_I2.4411.00
86_E115_Y2.3891.00
456_K459_D2.3641.00
104_M108_H2.3591.00
117_D223_R2.3541.00
452_Y456_K2.3441.00
64_S106_L2.3141.00
119_M223_R2.2891.00
218_K427_E2.2661.00
242_L458_F2.2621.00
213_R457_A2.2330.99
134_A144_L2.2000.99
141_I223_R2.1830.99
2_C74_N2.1690.99
412_T419_A2.1480.99
385_S388_E2.0520.99
71_L124_L2.0370.99
90_Y107_K2.0080.99
117_D427_E1.9770.98
241_S306_V1.9710.98
108_H115_Y1.9690.98
65_S90_Y1.9660.98
506_M529_L1.9440.98
230_Y363_M1.9290.98
212_I432_C1.8600.97
65_S103_P1.8580.97
215_S431_L1.8520.97
288_H315_H1.8480.97
230_Y362_K1.8450.97
142_T413_M1.8220.97
241_S470_L1.7590.96
124_L133_V1.7590.96
24_S46_V1.7570.96
216_L243_I1.7490.96
304_R308_K1.7390.96
329_D533_A1.7370.96
322_Q326_D1.7290.96
80_H225_M1.7090.95
117_D426_R1.6880.95
102_I106_L1.6850.95
368_E407_R1.6840.95
216_L246_I1.6570.94
26_R167_D1.6320.93
366_S419_A1.6190.93
368_E408_A1.6170.93
507_F534_L1.6140.93
349_M368_E1.6060.93
302_A477_R1.5930.92
217_E249_R1.5760.92
159_A164_C1.5510.91
374_F432_C1.5330.90
125_Y130_D1.5260.90
355_K358_A1.5210.90
345_A349_M1.5200.90
352_L356_I1.5170.90
159_A165_L1.5160.89
349_M408_A1.4940.89
73_V98_C1.4880.88
498_A526_W1.4680.87
71_L98_C1.4580.87
63_T70_M1.4560.87
404_D408_A1.4550.87
123_T134_A1.4540.87
343_I407_R1.4500.87
212_I374_F1.4490.86
50_L61_P1.4460.86
375_G441_M1.4430.86
32_P50_L1.4410.86
219_A223_R1.4390.86
92_F100_P1.4330.86
112_A133_V1.4290.85
213_R460_T1.4250.85
134_A158_F1.4180.85
69_Y102_I1.4140.85
192_F430_Q1.4100.84
246_I469_Y1.4010.84
50_L60_Q1.3970.84
63_T67_G1.3900.83
232_V244_A1.3900.83
60_Q74_N1.3880.83
231_G361_V1.3860.83
64_S67_G1.3840.83
138_P426_R1.3830.83
503_S506_M1.3700.82
373_I429_L1.3580.81
94_T99_E1.3560.81
459_D476_W1.3540.81
34_W51_A1.3500.81
515_G518_I1.3490.81
330_D354_R1.3450.80
64_S69_Y1.3420.80
299_D485_G1.3420.80
243_I475_L1.3370.80
61_P74_N1.3360.80
357_K362_K1.3350.80
145_Y410_K1.3340.80
146_M173_T1.3330.80
546_R554_G1.3320.79
33_D36_G1.3300.79
33_D61_P1.3190.79
50_L74_N1.3130.78
180_V189_T1.3130.78
49_R52_I1.3070.78
103_P106_L1.2970.77
64_S103_P1.2970.77
2_C76_E1.2910.76
370_S423_F1.2890.76
221_R250_E1.2880.76
5_F23_L1.2860.76
30_R50_L1.2840.76
69_Y106_L1.2790.75
503_S507_F1.2770.75
349_M407_R1.2750.75
289_S314_H1.2710.75
54_G227_E1.2710.75
242_L454_L1.2700.75
74_N418_E1.2670.74
104_M116_L1.2600.74
52_I74_N1.2390.72
33_D50_L1.2370.72
73_V77_I1.2330.72
306_V474_I1.2250.71
177_P243_I1.2230.71
64_S90_Y1.2170.70
142_T409_N1.2030.69
233_L408_A1.2010.69
20_A46_V1.1990.69
68_E106_L1.1960.68
286_S313_I1.1850.67
120_F135_A1.1810.67
49_R74_N1.1780.67
526_W530_K1.1760.66
460_T472_E1.1760.66
84_R92_F1.1690.66
103_P107_K1.1670.66
516_S542_D1.1670.66
32_P36_G1.1620.65
47_H62_I1.1600.65
146_M188_I1.1560.64
211_A215_S1.1540.64
2_C52_I1.1540.64
327_A351_L1.1510.64
299_D404_D1.1500.64
343_I368_E1.1500.64
299_D407_R1.1480.64
205_T456_K1.1420.63
356_I364_V1.1420.63
49_R58_G1.1420.63
548_I554_G1.1390.63
149_S155_T1.1390.63
105_Y124_L1.1370.63
289_S312_S1.1360.62
428_F432_C1.1360.62
174_A543_T1.1350.62
435_I453_I1.1340.62
60_Q76_E1.1310.62
179_H430_Q1.1280.62
223_R421_V1.1230.61
553_W557_E1.1210.61
47_H413_M1.1200.61
85_E88_A1.1190.61
520_T557_E1.1180.61
354_R358_A1.1140.60
244_A312_S1.1140.60
504_D522_K1.1140.60
554_G557_E1.1120.60
61_P76_E1.1070.59
481_Q485_G1.1010.59
461_T467_K1.0930.58
552_D555_C1.0900.58
162_L248_A1.0890.58
2_C61_P1.0880.57
343_I349_M1.0870.57
30_R33_D1.0850.57
80_H84_R1.0850.57
311_G373_I1.0840.57
33_D60_Q1.0820.57
364_V419_A1.0810.57
222_K225_M1.0780.56
217_E220_V1.0770.56
27_I164_C1.0760.56
208_D435_I1.0750.56
509_S512_A1.0750.56
65_S107_K1.0740.56
215_S427_E1.0720.56
220_V246_I1.0710.56
426_R430_Q1.0710.56
368_E421_V1.0700.56
32_P61_P1.0700.56
356_I375_G1.0700.56
205_T452_Y1.0690.55
292_I368_E1.0660.55
29_H33_D1.0650.55
213_R458_F1.0620.55
548_I553_W1.0590.54
457_A475_L1.0590.54
113_P122_W1.0580.54
301_Q305_K1.0560.54
538_K541_A1.0560.54
224_L291_A1.0560.54
17_K21_L1.0470.53
2_C60_Q1.0440.53
238_L455_R1.0430.53
50_L418_E1.0430.53
58_G96_S1.0430.53
455_R476_W1.0420.53
2_C50_L1.0420.53
175_F181_Y1.0420.53
90_Y102_I1.0420.53
289_S316_E1.0400.52
113_P133_V1.0400.52
369_G424_L1.0400.52
488_Y491_I1.0390.52
366_S421_V1.0350.52
387_A391_T1.0330.52
344_R368_E1.0320.52
31_G50_L1.0320.52
344_R379_Y1.0320.52
339_D368_E1.0280.51
556_A564_A1.0260.51
474_I489_S1.0220.50
352_L355_K1.0210.50
349_M404_D1.0210.50
53_V80_H1.0210.50
166_T538_K1.0200.50
138_P141_I1.0200.50
68_E128_K1.0180.50
552_D557_E1.0170.50
304_R307_A1.0170.50
497_T501_V1.0150.50
486_V491_I1.0120.49
218_K221_R1.0110.49
81_I225_M1.0080.49
92_F99_E1.0070.49
408_A419_A1.0070.49
33_D47_H1.0070.49
68_E90_Y1.0050.49
344_R407_R1.0050.49
546_R549_P1.0040.49
2_C30_R1.0040.49
339_D407_R1.0000.48
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
1ct9A40.87411000.277Contact Map
1q15A40.79551000.457Contact Map
1jgtA20.79551000.484Contact Map
1ao0A40.64511000.757Contact Map
1xffA20.34271000.775Contact Map
1ecfA20.70281000.786Contact Map
1te5A20.30591000.787Contact Map
4amvA30.83741000.788Contact Map
3mdnA40.29021000.817Contact Map
4zflA1201000.825Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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