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ASN2

ID: 1515180499 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 572 (546)
Sequences: 955 (461)
Seq/Len: 1.749
Nf(neff/√len): 19.7

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 1.749).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
303_R315_E4.1901.00
304_K472_E3.5751.00
34_W59_A3.3761.00
141_V420_V3.2971.00
90_Y103_P3.2951.00
117_D222_K2.7371.00
119_M141_V2.7361.00
64_S68_E2.6991.00
139_I428_L2.6991.00
287_H312_I2.6981.00
455_K458_D2.4471.00
20_A44_I2.4311.00
451_Y455_K2.4211.00
104_L108_H2.3991.00
117_D223_R2.3651.00
86_M115_Y2.3471.00
119_M223_R2.3111.00
64_S106_L2.2631.00
218_K426_D2.2621.00
242_L457_F2.2591.00
213_R456_A2.1670.99
141_V223_R2.1660.99
411_T418_A2.1660.99
2_C74_N2.1500.99
384_S387_E2.1460.99
134_A144_L2.1200.99
108_H115_Y2.0490.99
71_L124_L1.9860.98
90_Y107_E1.9690.98
241_S305_V1.9450.98
117_D426_D1.9380.98
65_A90_Y1.9370.98
505_M528_L1.9370.98
230_Y362_M1.9310.98
215_S430_L1.9210.98
287_H314_H1.8870.98
124_L133_V1.8840.98
230_Y361_K1.8680.98
65_A103_P1.8380.97
142_V412_M1.8370.97
212_I431_C1.8120.97
24_S46_V1.7730.96
241_S469_L1.7410.96
328_D532_A1.7350.96
216_L246_I1.7160.95
216_L243_I1.7130.95
102_I106_L1.7070.95
117_D425_K1.6970.95
80_H225_M1.6920.95
367_E406_R1.6780.95
321_Q325_D1.6280.93
506_F533_W1.6210.93
159_A165_L1.6200.93
367_E407_A1.6160.93
348_M367_E1.6150.93
217_E249_R1.6060.93
26_K167_D1.6000.93
301_A476_R1.5820.92
159_A164_C1.5760.92
365_S418_A1.5680.92
303_R307_K1.5470.91
354_K357_A1.5390.91
545_R553_G1.5230.90
348_M407_A1.5060.89
112_A133_V1.5030.89
351_L355_I1.5020.89
63_T70_M1.4990.89
374_G440_M1.4920.89
125_Y130_D1.4830.88
73_V98_C1.4830.88
63_T67_G1.4810.88
344_A348_M1.4770.88
71_L98_C1.4680.88
32_P50_L1.4640.87
219_A223_R1.4620.87
92_F100_P1.4580.87
403_D407_A1.4500.87
69_Y102_I1.4470.87
342_I406_R1.4440.86
497_A525_W1.4360.86
138_P425_K1.4330.86
64_S67_G1.4320.86
123_C134_A1.4310.86
213_R459_T1.4300.86
212_I373_F1.4270.86
134_A158_F1.4210.85
246_I468_Y1.4180.85
50_L61_P1.4140.85
242_L453_L1.4130.85
232_V244_A1.3980.84
231_G360_V1.3920.84
372_I428_L1.3800.83
34_W51_A1.3730.82
50_L60_Q1.3710.82
60_Q74_N1.3700.82
329_D353_R1.3690.82
373_F431_C1.3650.82
145_Y409_K1.3610.82
94_T99_E1.3550.81
458_D475_W1.3540.81
298_D484_G1.3470.81
103_P106_L1.3240.79
356_K361_K1.3190.79
69_Y106_L1.3180.79
61_P74_N1.3140.78
180_V189_T1.3120.78
514_G517_I1.3110.78
33_D36_G1.3010.77
192_F429_Q1.3000.77
243_I474_L1.2990.77
221_R250_E1.2950.77
50_L74_N1.2920.77
33_D61_P1.2870.76
104_L116_L1.2840.76
49_R52_I1.2840.76
2_C76_E1.2830.76
64_S69_Y1.2820.76
348_M406_R1.2730.75
562_Y570_V1.2650.75
305_V473_I1.2620.74
369_S422_F1.2570.74
74_N417_E1.2530.74
64_S103_P1.2520.74
20_A46_V1.2480.73
52_I74_N1.2450.73
73_V77_I1.2450.73
288_S313_H1.2340.72
54_G227_E1.2330.72
285_R312_I1.2330.72
68_E106_L1.2200.71
30_R50_L1.2130.70
502_S506_F1.2120.70
233_L407_A1.2090.70
5_F23_L1.2030.69
33_D50_L1.2000.69
120_F135_A1.2000.69
103_P107_E1.1910.68
146_M173_I1.1890.68
49_R74_N1.1860.68
179_H429_Q1.1850.68
2_C52_I1.1840.67
32_P36_G1.1770.67
205_T455_K1.1700.66
502_S505_M1.1660.66
162_L248_A1.1640.66
105_Y124_L1.1640.66
223_R420_V1.1630.65
211_A215_S1.1620.65
142_V408_N1.1610.65
342_I367_E1.1560.65
64_S90_Y1.1540.65
146_M188_I1.1540.65
47_H62_I1.1530.64
525_W529_K1.1530.64
84_R92_F1.1510.64
355_I363_V1.1500.64
434_I452_I1.1450.64
174_S542_T1.1440.64
298_D403_D1.1440.64
49_R58_G1.1360.63
60_Q76_E1.1360.63
298_D406_R1.1350.63
547_I553_G1.1350.63
113_P133_V1.1320.62
459_T471_E1.1290.62
547_I552_W1.1280.62
326_A350_L1.1270.62
519_T556_E1.1270.62
177_P243_I1.1270.62
149_S155_T1.1190.61
215_S426_D1.1180.61
460_T466_K1.1110.60
515_D541_D1.1110.60
208_D434_I1.1100.60
47_H412_M1.1090.60
353_R357_A1.1080.60
425_K429_Q1.1070.60
244_A311_S1.1040.59
61_P76_E1.1040.59
473_I488_S1.1030.59
32_P61_P1.1020.59
480_Q484_G1.1010.59
217_E220_V1.1010.59
80_H84_R1.0970.59
90_Y102_I1.0960.59
85_E88_S1.0960.59
456_A474_L1.0930.58
205_T451_Y1.0890.58
288_S311_S1.0890.58
310_G372_I1.0890.58
288_S315_E1.0890.58
220_V246_I1.0840.57
427_F431_C1.0820.57
552_W556_E1.0800.57
224_L290_A1.0760.57
138_P141_V1.0740.56
551_D554_C1.0740.56
367_E420_V1.0690.56
553_G556_E1.0690.56
342_I348_M1.0670.56
238_L454_R1.0670.56
545_R548_P1.0630.55
300_Q304_K1.0610.55
2_C61_P1.0590.55
113_P122_F1.0580.55
29_H33_D1.0550.54
291_I367_E1.0540.54
33_D60_Q1.0540.54
2_C30_R1.0520.54
175_F181_Y1.0510.54
58_G96_S1.0510.54
65_A107_E1.0470.53
27_I164_C1.0470.53
363_V418_A1.0430.53
45_F100_P1.0430.53
412_M563_A1.0430.53
309_I415_G1.0400.53
81_I225_M1.0400.53
338_D367_E1.0390.53
213_R457_F1.0370.52
454_R475_W1.0370.52
92_F99_E1.0350.52
348_M403_D1.0340.52
343_R367_E1.0340.52
219_A291_I1.0290.51
222_K225_M1.0260.51
166_T537_K1.0260.51
303_R306_A1.0260.51
487_Y490_I1.0230.51
17_K21_L1.0230.51
50_L417_E1.0200.51
218_K221_R1.0200.51
343_R378_Y1.0190.50
216_L422_F1.0170.50
2_C50_L1.0170.50
110_I189_T1.0160.50
2_C60_Q1.0160.50
503_D521_K1.0160.50
368_G423_L1.0140.50
508_N511_A1.0100.49
496_T500_A1.0090.49
147_G165_L1.0090.49
386_A390_T1.0090.49
343_R406_R1.0080.49
407_A418_A1.0080.49
365_S420_V1.0030.49
159_A213_R1.0020.49
537_K540_A1.0020.49
216_L242_L1.0020.49
60_Q97_D1.0010.48
31_G50_L1.0000.48
233_L355_I1.0000.48
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
1ct9A40.87411000.277Contact Map
1q15A40.79721000.455Contact Map
1jgtA20.79721000.482Contact Map
1ao0A40.64511000.757Contact Map
1xffA20.34271000.775Contact Map
1ecfA20.66261000.785Contact Map
1te5A20.30591000.787Contact Map
4amvA30.84271000.79Contact Map
3mdnA40.29021000.817Contact Map
4zflA1201000.825Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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