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SLC35F2

ID: 1515509291 Query Sequence

Download Alignment
We filter this alignment to remove positions that have > 75% gaps before running GREMLIN.
Length: 374 (307)
Sequences: 10828 (7572.7)
Seq/Len: 35.270
Nf(neff/√len): 432.2

HHblits Results: (2016_02)
Sequences per length for each position, BEFORE the coverage/gap filter was applied

Legend: Dots above the red line, indicates positions where there is > 5 sequences per length (if visible, green bar = 20 seq/len). Point of this graphic is to give you an idea if resubmitting a sub-portion of your sequence might help increase the overall Seq/Len (which after applying the coverage filter is at: 35.270).

Sequences per length for each position, AFTER the coverage/gap filter was applied
Legend: If visible, the positions blocked in grey are regions that have > 75% gaps and were automatically trimmed and excluded in the GREMLIN analysis.

Trim query Generate MSA using: Filter MSA GREMLIN
Start: End: E-value: Iterations: Coverage: Remove gaps: Prior:

GREMLIN Results:
Residue pairs sorted by coevolution strength:

Legend: The darker and larger the blue dots, the higher strength in covariance. Below we provide the list of the top [1.5 x length] covarying residues with sequence seperation > 3. The i and j are positions as given in the input sequence.

ij Scaled score Prob
128_A202_V3.5481.00
167_A328_G2.7891.00
149_M168_V2.6981.00
281_L285_V2.6631.00
46_G282_V2.6511.00
200_I204_L2.6511.00
124_V204_L2.5181.00
45_L281_L2.5001.00
84_F237_T2.4731.00
84_F234_L2.3921.00
158_A217_E2.3471.00
147_V206_A2.3361.00
124_V201_L2.3021.00
88_T230_G2.1571.00
81_C237_T2.1541.00
281_L284_K2.1211.00
77_F241_G2.1131.00
136_S199_D2.1111.00
127_R201_L2.1001.00
41_K285_V2.0991.00
306_G312_Y2.0571.00
196_L200_I2.0171.00
132_T198_G1.9491.00
153_W160_Y1.9061.00
79_N272_M1.8741.00
54_C301_Y1.8591.00
140_L202_V1.7971.00
131_Y197_I1.7691.00
177_T324_V1.7631.00
104_L107_K1.7511.00
152_S158_A1.7281.00
51_L301_Y1.7241.00
38_N41_K1.6941.00
171_C296_L1.6831.00
287_S290_S1.6681.00
132_T199_D1.6671.00
44_A48_M1.6581.00
124_V208_L1.6131.00
115_G235_F1.6101.00
147_V210_A1.6031.00
83_L233_G1.6021.00
316_G320_L1.5851.00
60_S126_V1.5741.00
45_L278_F1.5621.00
151_L214_V1.5621.00
143_F147_V1.5561.00
151_L155_I1.5311.00
297_T301_Y1.5241.00
169_A173_L1.5241.00
134_L306_G1.5221.00
171_C328_G1.5191.00
283_I287_S1.5041.00
330_I334_S1.5021.00
54_C305_V1.4941.00
55_G265_F1.4891.00
58_I305_V1.4831.00
167_A332_Y1.4831.00
218_Y221_K1.4731.00
301_Y305_V1.4611.00
173_L177_T1.4401.00
49_L282_V1.4371.00
40_L44_A1.4231.00
77_F81_C1.4201.00
147_V207_S1.4111.00
110_K219_I1.4111.00
48_M278_F1.4000.99
165_F169_A1.3960.99
314_F319_I1.3960.99
174_G325_I1.3940.99
133_T136_S1.3920.99
151_L210_A1.3920.99
177_T317_L1.3910.99
148_L288_A1.3900.99
152_S156_L1.3810.99
132_T136_S1.3790.99
174_G321_S1.3700.99
61_Q312_Y1.3630.99
161_R164_H1.3570.99
140_L203_L1.3570.99
127_R130_Q1.3560.99
137_V202_V1.3510.99
286_T290_S1.3480.99
148_L210_A1.3360.99
47_Q297_T1.3330.99
77_F237_T1.3260.99
138_Q302_S1.3180.99
140_L206_A1.3170.99
88_T234_L1.3130.99
131_Y201_L1.3070.99
162_V166_I1.3040.99
82_L267_A1.3000.99
224_S227_E1.2980.99
238_I242_I1.2860.99
163_I331_L1.2850.99
120_E208_L1.2800.99
37_W41_K1.2730.99
109_W113_L1.2700.99
174_G328_G1.2650.99
84_F88_T1.2630.99
178_M321_S1.2590.99
128_A132_T1.2560.99
148_L214_V1.2340.98
319_I323_T1.2310.98
89_V92_A1.2170.98
305_V309_L1.2120.98
160_Y165_F1.2020.98
323_T327_V1.1970.98
53_I279_M1.1920.98
84_F230_G1.1910.98
197_I201_L1.1750.98
216_E280_P1.1710.98
307_L314_F1.1690.97
134_L310_F1.1610.97
118_D212_S1.1530.97
147_V203_L1.1530.97
86_I273_F1.1490.97
196_L199_D1.1460.97
162_V165_F1.1440.97
106_R110_K1.1430.97
156_L214_V1.1370.97
334_S338_R1.1260.97
173_L324_V1.1230.97
77_F244_L1.1140.96
316_G319_I1.1100.96
257_W261_I1.1070.96
153_W159_R1.1010.96
117_A211_I1.0980.96
154_F157_H1.0960.96
151_L211_I1.0950.96
127_R131_Y1.0920.96
163_I334_S1.0860.96
198_G202_V1.0800.95
213_N283_I1.0760.95
43_I294_G1.0700.95
135_T303_L1.0690.95
282_V291_V1.0660.95
286_T291_V1.0650.95
112_I235_F1.0570.95
267_A273_F1.0550.95
110_K218_Y1.0490.94
180_G315_S1.0400.94
149_M153_W1.0390.94
107_K222_K1.0370.94
139_L179_V1.0370.94
151_L154_F1.0320.94
107_K223_L1.0310.94
113_L215_C1.0300.94
170_V328_G1.0280.94
315_S319_I1.0270.94
131_Y198_G1.0250.93
53_I275_L1.0230.93
104_L108_W1.0190.93
110_K215_C1.0160.93
258_D261_I1.0160.93
315_S318_Y1.0140.93
197_I200_I1.0140.93
76_S126_V1.0130.93
87_Y273_F1.0120.93
125_I129_Y1.0110.93
105_K109_W1.0030.93
Legend: Only predictions with Scaled Score > 1 are listed. For all 3L/2 predictions, click on one of the links below.
Prob = P(contact | scaled_score, seq/len).
Scaled Score = (raw_score/average(raw_scores)).
NEW Features
HHsearch Results: (06Aug15)
GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled Score > 1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-position alignment confidence. Inter oligomeric contacts in the pdb are in shades of red.
PDB M Cov P(%) HHΔ  
2i68A20.021498.70.838Contact Map
3b5dA20.01698.10.867Contact Map
4j05A20.887763.40.941Contact Map
4pypA10.858354.60.944Contact Map
4tphA20.804833.20.951Contact Map
3wdoA10.823532.50.951Contact Map
4zw9A1021.50.955Contact Map
4ldsA20.532120.30.956Contact Map
2cfqA10.553520.20.956Contact Map
1pw4A10.88516.30.958Contact Map
M = number of chains, if homo-oligomer
Cov = coverage of hit
P(%) = probability

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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